is a facultative intracellular pathogen that can survive and grow in macrophages by preventing phagolysosomal fusion. In this study cassette mutagenesis was used to generate a library of insertion mutants of Two related mutants, KM14 and KM14S, initially identified as defective for growth in macrophages, were found to be sensitive to serum. These mutants were also found to grow approximately 1000-fold less well in the livers and spleens of infected mice. We cloned a genetic locus that was presumably mutagenized in these mutants and found that it included genes that had high similarity in their deduced amino acid sequence to those of and of The former is a member of the superfamily of ABC transporter proteins. We named the corresponding genes in , Integration of a cloned locus into the chromosome of KM14S partially restored the serum resistance phenotype found in wild-type


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error