1887

Abstract

sp. strain ATCC 29133 (PCC 73102; 29133) is a symbiotically-competent, facultatively heterotrophic, diazotrophic cyanobacterium with the capacity to differentiate specialized cells such as heterocysts, akinetes and hormogonial filaments. We have optimized several methods for physiological and molecular genetic analysis of 29133. By use of a Tnderivative, Tn-1063 (KmBmSm), delivered by conjugation from , antibiotic-resistant mutants of 29133 were generated at a frequency of approximately 1 × 10, 0.4% of which expressed a nitrogen fixation (heterocyst) defective phenotype. Mutant strain UCD 328 was isolated after co-culture of 86 29133::Tn-1063 clones with the symbiotic plant partner, ; strain UCD 328 expressed a symbiotic phenotype of increased frequency of hormogonia-dependent infection. The transposon and flanking genomic DNA was recovered from strain UCD 328, the mutation and phenotype reconstructed by homologous recombination in 29133, and the transposition site identified from a 29133 genomic library. Transposon mutagenesis has thus provided the means for isolation and identification of developmental and symbiotic-specific genes of 29133.

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1994-12-01
2021-10-26
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References

  1. Allen M.B., Arnon D.I. Studies on nitrogen-fixing blue-green algae I Growth and nitrogen fixation by Anabaena cylindrica Lemm. Plant Physiol 1955; 30:366–372
    [Google Scholar]
  2. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. Current Protocols in Molecular Biology 1987 New York: Green Publishing Associates & Wiley-Interscience;
    [Google Scholar]
  3. Better M., Helinski D.R. Isolation and characterization of the recA gene of Rhizobium meliloti. J Bacteriol 1983; 155:311–316
    [Google Scholar]
  4. Black T.A., Wolk C.P. Analysis of a Het-mutation in Anabaena sp. strain PCC 7120 implicates a secondary metabolite in the regulation of heterocyst spacing. J Bacteriol 1994; 176:2282–2292
    [Google Scholar]
  5. Black T.A., Cai Y., Wolk C.P. Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol Microbiol 1993; 9:77–84
    [Google Scholar]
  6. Braun A.C., Wood H.N. On activation of certain essential biosynthetic systems in cells of Vinca rosea L. Proc Natl Acad Sei USA 1962; 48:1776–1782
    [Google Scholar]
  7. Buikema W.J., Haselkorn R. Isolation and complementation of nitrogen fixation mutants of the cyanobacterium Anabaena sp strain PCC 7120. J Bacteriol 1991; 173:1879–1885
    [Google Scholar]
  8. Buikema W.J., Haselkorn R. Molecular genetics of cyanobacterial development. Annu Rep Plant Physiol Plant Mol Biol 1993; 44:33–52
    [Google Scholar]
  9. Cai Y., Wolk C.P. Use of a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion sequences. J Bacteriol 1990; 172:3138–3145
    [Google Scholar]
  10. Campbell E.L., Meeks J.C. Characteristics of hor-mogonia formation by symbiotic Nostoc spp. in response to the presence of Anthoceros punctatus or its extracellular products. Appl Environ Microbiol 1989; 55:125–131
    [Google Scholar]
  11. Campbell E.L., Meeks J.C. Evidence for plant-mediated regulation of nitrogenase expression in the Anthoceros-Nostoc symbiotic association. J Gen Microbiol 1992; 138:473–480
    [Google Scholar]
  12. Cardemil L., Wolk C.P. The polysaccharides from heterocyst and spore envelopes of a blue-green algae. J Biol Chem 1976; 251:2967–2975
    [Google Scholar]
  13. Cardemil L., Wolk C.P. Polysaccharides from the envelopes of heterocysts and spores of the blue-green algae Anabaena variabilis and Cylindrospermum licheniforme. J Phycol 1981; 17:234–240
    [Google Scholar]
  14. Damerval T., Guglielmi G., Houmard J., Tandeau de Marsac N. Hormogonium formation in the cyanobacterium Calothrix: a photoregulated developmental process. Plant Cell 1991; 3:191–201
    [Google Scholar]
  15. Elhai J., Wolk C.P. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 1988a; 68:119–138
    [Google Scholar]
  16. Elhai J., Wolk C.P. Conjugal transfer of DNA to cyanobacteria. Methods Enzymol 1988b; 167:747–754
    [Google Scholar]
  17. Enderlin C.S., Meeks J.C. Pure culture and reconstitution of the Anthoceros-Nostoc symbiotic association. Planta 1983; 158:157–165
    [Google Scholar]
  18. Ernst A., Black T., Cai Y., Panoff J.-M., Tiwari D.N., Wolk C.P. Synthesis of nitrogenase in mutants of the cyanobacterium Anabaena sp. strain PCC 7120 affected in heterocyst development or metabolism. J Bacteriol 1992; 174:6025–6032
    [Google Scholar]
  19. Felkner R.H., Barnum S.R. Plasmid content and homology of 16 strains of filamentous, nonheterocystous cyanobacteria. Curr Microbiol 1988; 17:37–41
    [Google Scholar]
  20. Figurski D.H., Helinski D.R. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci USA 1979; 76:1648–1652
    [Google Scholar]
  21. Figurski D., Meyer R., Miller D.S., Helinski D.R. Generation in vitro of deletions in the broad host range plasmid RK2 using phage Mu insertions and a restriction endonuclease. Gene 1976; 1:107–119
    [Google Scholar]
  22. Grant S.G., Jesse J., Bloom F.R., Hanahan D. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc Natl Acad Sci USA 1990; 87:4645–4649
    [Google Scholar]
  23. Herdman M. Akinetes: structure and function. In The Cjanobacteria 1987 Edited by Fay P., Van Baalen C. Amsterdam: Elsevier; pp 227–250
    [Google Scholar]
  24. Herdman M., Rippka R. Cellular differentiation: hormogonia and baeocytes. Methods Enyymol 1988; 167:232–242
    [Google Scholar]
  25. Hirosawa T., Wolk C.P. Isolation and characterization of a substance which stimulates the formation of akinetes in the cyanobacterium Cylindrospermum licheniforme Kutz. J Gen Microbiol 1979; 114:433–441
    [Google Scholar]
  26. Houmard J., Tandeau de Marsac N. Cyanobacterial genetic tools: current status. Methods Enzymol 1988; 167:808–847
    [Google Scholar]
  27. Ish-Horowicz D., Burke J.F. Rapid and efficient cosmid cloning. Nucleic Acids Res 1981; 9:2989–2998
    [Google Scholar]
  28. Johansson C., Bergman B. Reconstitution of the Gunnera manicata Linden symbiosis: cyanobacterial specificity. New Phytol 1994; 126:643–652
    [Google Scholar]
  29. Lambert G.R., Carr N.G. Resistance of DNA from filamentous and unicellular cyanobacteria to restriction endonuclease cleavage. Biochim Biophys Acta 1984; 781:45–55
    [Google Scholar]
  30. Meeks J.C. Cyanobacterial-bryophyte associations. In Symbiotic Cyanobacteria 1990 Edited by Rai A.N. Boca Raton, FL: CRC Press; pp 43–63
    [Google Scholar]
  31. Meeks J.C., Wycoff K.L., Chapman J.S., Enderlin C.S. Regulation of expression of nitrate and dinitrogen assimilation by Anabaena species. Appl Environ Microbiol 1983; 45:1351–1359
    [Google Scholar]
  32. Meeks J.C., Enderlin C.S., Joseph C.M., Chapman J.S., Lollar M.W.L. Fixation of [I3N]N2 and transfer of fixed nitrogen in the Anthoceros-Nostoc symbiotic association. Planta 1985; 164:406–414
    [Google Scholar]
  33. Potts M. Isolation of plasmids from cyanobacteria. BRL Focus 1989; 6:4–6
    [Google Scholar]
  34. Raleigh E.A. Organization and function of the mcrBC genes of Escherichia coli K-12. Mol Microbiol 1992; 6:1079–1086
    [Google Scholar]
  35. Rippka R., Deruelles J., Waterbury J.B., Herdman M., Stanier R.Y. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 1979; 111:1–61
    [Google Scholar]
  36. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: a Laboratory Manual, 2nd edn 1989 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  37. Shestakov S.V., Reaston J. Gene transfer and host-vector systems of cyanobacteria. Oxford Surv Plant Mol Cell Biol 1987; 4:137–166
    [Google Scholar]
  38. Steinberg N.A., Meeks J.C. Physiological sources of reductant for nitrogen fixation activity of Nostoc sp. strain UCD 7801 in symbiotic association with Anthoceros punctatus. J Bacteriol 1991; 173:6227–6233
    [Google Scholar]
  39. Stuttzman K., Gendel S.M. A rapid method for identifying plasmids in cyanobacteria. FEMS Microbiol Lett 1985; 26:337–348
    [Google Scholar]
  40. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 1982; 19:259–268
    [Google Scholar]
  41. Wallis J.G. Development of genetic tools for the cyanobacterium Nostoc sp. strain ATCC 29133 1993 PhD thesis, University of California, Davis;
    [Google Scholar]
  42. Wolk C.P. Heterocysts. In The Biology of Cyanobacteria 1982 Edited by Carr N.G., Whitton B.A. Oxford: Blackwell Scientific Publications; pp 359–386
    [Google Scholar]
  43. Wolk C.P. Genetic analysis of cyanobacterial development. Curr Opin Genet Dev 1991; 1:336–339
    [Google Scholar]
  44. Wolk C.P., Vonshank A., Kehoe P., Elhai J. Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen fixing filamentous cyanobacteria. Proc Natl Acad Sci USA 1984; 81:5355–5359
    [Google Scholar]
  45. Wolk C.P., Cai Y., Panoff J.-M. Use of a transposon with luciferase as a reporter to identify environmentally responsive genes in a cyanobacterium. Proc Natl Acad Sci USA 1991; 88:5355–5359
    [Google Scholar]
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