1887

Abstract

Summary: Esterases of 85 strains of the four biochemically-defined subgenera of , when analysed by the acrylamide-agarose zymogram technique using several synthetic substrates, gave four principal bands (E, E, E, E) and two minor ones. The E esterase band hydrolysed α-naphthyl acetate, whereas the E band hydrolysed -naphthyl acetate. These bands were resistant to di-isofluoropropyl phosphate (DFP) and their electrophoretic distribution among the strains occurred within a relatively small range, being the distance moved by the esterase band as a percentage of the distance moved by the dye front. The E band hydrolysed α-naphthyl acetate and α-naphthyl butyrate and, to a lesser degree, -naphthyl esters, whereas the E band hydrolysed α-naphthyl acetate. These bands were sensitive to DFP and their electrophoretic distribution among the strains occurred in a wide range. All Salmonella strains were closely related in terms of their esterase profiles. However, the divergences in electrophoretic distribution of bands E and E were sufficient to recognize the subgenera of most of the Salmonella strains analysed.

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/content/journal/micro/10.1099/00221287-98-2-535
1977-02-01
2021-10-23
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