1887

Abstract

Summary: Compact-colony forming active substance (CCFAS), the material responsible for the compact colonies of observed in serum soft agar, was found to be an alkaline-stable, associated polysaccharide containing galactose, -acetylglucosamine, ribitol, phosphorus and a small quantity of alanine. This substance, when extracted from strains unable to produce protein A and clumping factor, was able to absorb the serum-reacting factor whereas a teichoic acid preparation of one strain could not. The formation of CCFAS was unaffected by the age of the cells, whereas when staphylococci were cultured at alkaline pH, young cells produced more clumping factor than old ones. Both fibrinogen and its degradation products were capable of inducing compact colonies in a strain of The ability of human sera to interact in compact-colony formation was independent of the immunoglobulin content. Thus neither protein A, clumping factor, nor teichoic acid participate in the CCFAS reaction.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-98-1-67
1977-01-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/98/1/mic-98-1-67.html?itemId=/content/journal/micro/10.1099/00221287-98-1-67&mimeType=html&fmt=ahah

References

  1. Alami S.Y., Kelly F.C. 1959; Demonstration of staphylococcal clumping factor and free coagulase in soft agar media. Journal of Bacteriology 58:539–544
    [Google Scholar]
  2. Duthie E.S. 1955; The action of fibrinogen on certain pathogenic cocci. Journal of General Microbiology 13:383–436
    [Google Scholar]
  3. Elson L.A., Morgan W.T.J. 1953; A colormetric method for the determination of glucosamine and chondrosamine. Biochemical Journal 27:1828–1829
    [Google Scholar]
  4. Finkelstein R.A., Sulkin S.E. 1958; Characteristics of coagulase negative and coagulase positive staphylococci in serum-soft agar. Journal of Bacteriology 75:339–344
    [Google Scholar]
  5. Forsgren A., Sjöquist I. 1969; Protein A from Staphylococcus aureus. VII. Physicochemical and immunological characterization. Acta pathologica et microbiologica Scandinavica 27:466–481
    [Google Scholar]
  6. Forsum U., Forsgren A., Hjelm E. 1972; Role of protein A in the serum-soft agar technique. Infection and Immunity 6:583–586
    [Google Scholar]
  7. Iwata K., Eda T. 1968; Studies on unique staphylococcal strains exhibiting high virulence for mice by intraperitoneal inoculation. I. Morphological and biological properties of the strains. Japanese Journal of Bacteriology 23:165–171
    [Google Scholar]
  8. Jensen K. 1959 Undersölgelser over staphylococcerties antigenstruktur. Thesis Copenhagen: Munkgaard;
    [Google Scholar]
  9. Lipinski B., Hawiger J., Jeljaszewicz J. 1967; Staphylococcal clumping factor with soluble fibrin monomer complex. Journal of Experimental Medicine 126:979–988
    [Google Scholar]
  10. Matuno T., Slade H.D. 1970; Composition and properties of a group A streptococcal teichoic acid. Journal of Bacteriology 102:747–752
    [Google Scholar]
  11. Morris D.L. 1948; Quantitative determination of carbohydrates with Dreywood’s anthrone reagent. Science 107:234–255
    [Google Scholar]
  12. Siwecka M., Jeljaszewicz J. 1968; Purification and some properties of staphylococcal coagulase. Experimental Medicine and Microbiology 20:125–137
    [Google Scholar]
  13. Spiro R.G. 1959; Studies on the biosynthesis of glucosamine in the intact rat. Journal of Biological Chemistry 234:742–748
    [Google Scholar]
  14. Trevelyan W.E., Procter D.P., Harrison J.S. 1950; Detection of sugar on paper chromatograms. Nature; London: 166444–445
    [Google Scholar]
  15. Yoshida K. 1973; Compact-colony forming activity and the effect of pH on compact-type growth of Staphylococcus aureus strain in serum-soft agar. American Journal of Clinical Pathology 59:412–416
    [Google Scholar]
  16. Yoshida K., Smith M.R., Naito Y. 1970; Biological and immunological properties of encapsulated strains of Staphyloccus aureus from human sources. Infection and Immunity 2:582–532
    [Google Scholar]
  17. Yoshida K., Nakamura A., Ohtomo T., Iwami S. 1974; Detection of capsular antigen production in unencapsulated strains of Staphylococcus aureus. Infection and Immunity 9:620–623
    [Google Scholar]
  18. Yoshida K., Ohtomo T., Minegishi Y. 1975; Compact-colony forming active substance extracted from Staphylococcus aureus. Japanese Journal of Microbiology 19:75–76
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-98-1-67
Loading
/content/journal/micro/10.1099/00221287-98-1-67
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error