SUMMARY: Strains of that are unable to grow on fructose because they lack the phosphoenolpyruvate: fructose phosphotransferases specified by and mutate to grow on media containing fructose as sole carbon source, but do not regain the function of either of the missing phosphotransferases. Instead, fructose is taken up and phosphorylated to fructose 6-phosphate by a phosphoenolpyruvate: sorbitol phosphotransferase which, in wild-type cells, is induced by sorbitol but not by fructose, but which is constitutively expressed in these mutants. The regulatory gene controlling enzymes of sorbitol uptake and catabolism has been located on the genome as part of the linkage group


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