1887

Abstract

Summary: The addition of complex supplements (particularly amino acids) to cultures of growing on a good carbon source did not result in a substantial increase in the growth rate. Amino acids entered the cells within 30 s of addition and reached significant internal pool concentrations. Endogenous amino acid biosynthesis was quickly inhibited (about 75 %), with a substantial sparing of the original carbon source. Within 20 min of supplementation significant respiration of added amino acids was detected, yet the ATP pool size did not increase and the bacteria did not grow faster.

The RNA content of growing in complex medium differed from that of enteric bacteria in that, although it varied with growth rate, it was not substantially larger than the RNA content of bacteria grown in a minimal medium with a good carbon and energy source. The rate of RNA accumulation on shift-up remained substantially unchanged on supplementation if the minimal medium had a carbon source producing fast growth, and did not increase for about 30 min if the carbon source was relatively poor. In other respects RNA synthesis was similar to that of the enteric bacteria, being stringently controlled, inhibited by trimethoprim and continuing in the presence of chloramphenicol. It is proposed that growth of in complex media is limited by the rate of synthesis of stable RNA.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-93-2-227
1976-04-01
2021-08-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/93/2/mic-93-2-227.html?itemId=/content/journal/micro/10.1099/00221287-93-2-227&mimeType=html&fmt=ahah

References

  1. Abelson P.H. 1954; Amino acid biosynthesis in Escherichia coli: isotopic competition with 14C glucose. Journal of Biological Chemistry 206:335–343
    [Google Scholar]
  2. Abelson P.H., Bolton E.T., Aldous E. 1952; Utilization of carbon dioxide in the synthesis of proteins by Escherichia coli. I. Journal of Biological Chemistry 198:165–185
    [Google Scholar]
  3. Bremer H., Berry L., Dennis P.P. 1973; Regulation of ribonucleic acid synthesis in Escherichia coli B/r: an analysis of a shift up. II. Fraction of RNA polymerase engaged in the synthesis of stable RNA at different steady-state growth rates. Journal of Molecular Biology 75:161–179
    [Google Scholar]
  4. Cohen-Bazire G., Sistrom Q.R., Stanier R.Y. 1957; Kinetic studies of pigment synthesis by nonsulphur purple bacteria. Journal of Cellular and Comparative Physiology 49:25–68
    [Google Scholar]
  5. Cole H.A., Wimpenny J.W.T., Hughes D.E. 1967; The ATP pool in Escherichia coli. I. Measurement of the pool using a modified luciferase assay. Biochimica et biophysica acta 143:445–453
    [Google Scholar]
  6. Condon S. 1967 Cold-sensitive mutants of Pseudomonas putida. Ph.D. thesis University of California, Davis, U.S.A.:
    [Google Scholar]
  7. Condon S., Collins J.K., O’Donovan G.A. 1976; Regulation of arginine and pyrimidine biosynthesis in Pseudomonas putida.. Journal of General Microbiology 92:375–383
    [Google Scholar]
  8. Forrest W.W., Walker D.J. 1965; Synthesis of reserve materials for endogenous metabolism in Streptococcus faecalis. Journal of Bacteriology 89:1448–1452
    [Google Scholar]
  9. Fraenkel D.G., Neidhardt F.C. 1961; Use of chloramphenicol to study control of RNA synthesis in bacteria. Biochimica et biophysica acta 53:96–110
    [Google Scholar]
  10. Gryder R.M., Adams E. 1969; Inducible degradation of hydroxyproline in Pseudomonas putida:pathway regulation and hydroxyproline uptake. Journal of Bacteriology 97:292–306
    [Google Scholar]
  11. Heathcote J.G., Haworth C. 1969; An improved technique for the analysis of amino acids and related compounds on thin layers of cellulose. II. The quantitative determination of amino acids in protein hydrolysates. Journal of Chromatography 43:84–92
    [Google Scholar]
  12. Hegeman G.D. 1966; Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida.I. Synthesis of enzymes by the wild type. Journal of Bacteriology 91:1140–1154
    [Google Scholar]
  13. Jacoby G.A. 1964; The induction and repression of amino acid oxidation in Pseudomonas fluorescens. Biochemical Journal 92:1–8
    [Google Scholar]
  14. Kay W.W., Gronlund A.F. 1969; Amino acid pool formation in Pseudomonas aeruginosa. Journal of Bacteriology 97:282–291
    [Google Scholar]
  15. Kurland C.G., Maaløe O. 1962; Regulation of ribosomal and transfer RNA synthesis. Journal of Molecular Biology 4:193–210
    [Google Scholar]
  16. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  17. McGinnis J.F., Paigen K. 1969; Catabolite inhibition: a general phenomenon in the control of carbohydrate utilisation. Journal of Bacteriology 100:902–913
    [Google Scholar]
  18. Maaløe O., Kjeldgaard N.O. 1966 Control of Macromolecular Synthesis. New York and Amsterdam: W.A Benjamin;
    [Google Scholar]
  19. Marshall V.P., Sokatch J.R. 1972; Regulation of valine catabolism in Pseudomonas putida. Journal of Bacteriology 110:1073–1081
    [Google Scholar]
  20. Maurer R., Crawford I.P. 1971; New regulatory mutation affecting some of the tryptophan genes in Pseudomonas putida. Journal of Bacteriology 106:331–338
    [Google Scholar]
  21. Miovic M., Pizer L. 1971; Effect of trimethoprim on macromolecular synthesis in Escherichia coli. Journal of Bacteriology 106:856–862
    [Google Scholar]
  22. Munro H.N., Fleck A. 1966; The determination of nucleic acids. Methods of Biochemical Analysis 14:113–176
    [Google Scholar]
  23. Neidhardt F.C., Magasanik B. 1960; Studies on the role of ribonucleic acid in the growth of bacteria. Biochimica et biophysica acta 42:99–116
    [Google Scholar]
  24. Nierlich D.P. 1972a; Regulation of ribonucleic acid in growing bacterial cells. I. Control over the total rate of RNA synthesis. Journal of Molecular Biology 72:751–764
    [Google Scholar]
  25. Nierlich D.P. 1972b; Regulation of ribonucleic acid synthesis in growing bacterial cells. II. Control over the composition of the newly made RNA. Journal of Molecular Biology 72:765–777
    [Google Scholar]
  26. Palleroni N.J., Stanier R.Y. 1964; Regulatory mechanisms governing synthesis of the enzymes for tryptophan oxidation by Pseudomonas fluorescens. Journal of General Microbiology 35:319–334
    [Google Scholar]
  27. Robert-Gero M., Poiret M., Cohen G.N. 1970; The aspartate kinase of Pseudomonas putida.Regulation of synthesis and activity. Biochimica et biophysica acta 206:17–30
    [Google Scholar]
  28. Schaechter M., Maaløe O., Kjeldgaard N.O. 1958; Dependency on medium and temperature of cell size and chemical composition during balanced growth of Salmonella typhimurium. Journal of General Microbiology 19:592–606
    [Google Scholar]
  29. Stalon V., Ramos F., Pierard A., Wiame J.M. 1967; The occurrence of a catabolic and an anabolic ornithine carbamoyltransferase in Pseudomonas. Biochimica et biophysica acta 139:91–97
    [Google Scholar]
  30. Stanier R.Y., Palleroni N.J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. Journal of General Microbiology 43:159–271
    [Google Scholar]
  31. Udaka S. 1966; Pathway-specific pattern of control of arginine biosynthesis in bacteria. Journal of Bacteriology 91:617–627
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-93-2-227
Loading
/content/journal/micro/10.1099/00221287-93-2-227
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error