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Volume 90, Issue 2

Potassium Fluxes in Free

Abstract

SUMMARY: The unidirectional K fluxes across the mycelial surface of were determined using K. Influx was mediated by at least two kinetically distinct systems, one having an apparent of 6·5 -equiv. K/l and the other of about 1·0 m-equiv. K/1. The V for both systems was in the range 18 to 22 -equiv. K/100 mg mycelial dry matter/h (1· to 1·2 m-equiv. K/1 cell-water/min). Influx was strongly inhibited by 2,4-dinitrophenol, sodium azide, sodium arsenate and anaerobiosis. K efflux was dependent on the external Kconcentration and ranged from 3 to 10% of mycelial K/h. The maximum efflux rate was always considerably less than the initial influx rate for the Kconcentrations examined. During incubation in dilute KCl solutions, K influx decreased to a value approaching the K efflux rate. It is considered that equilibrium with external K is attained primarily by the regulation of Kinflux, and that this may be the principal mechanism controlling cytoplasmic K levels.

Adsorption of Kwas also observed throughout the Kconcentration range examined and can be attributed to two distinct K-binding entities at the mycelial surface, half-saturating at approximately 0·1 m- and 4·4 m-KCl respectively.

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  • Published Online:
© Society for General Microbiology, 1975
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1975-10-01
2024-04-26
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Potassium Fluxes in Neocosmospora vasinfecta
Microbiology 90, 311 (1975); https://doi.org/10.1099/00221287-90-2-311
/content/journal/micro/10.1099/00221287-90-2-311
/content/journal/micro/10.1099/00221287-90-2-311
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