1887

Abstract

SUMMARY: Among mutants of isolated from fluoroacetamide medium were some which synthesized amidase at about 5 % of the rate of the parent constitutive strain, 101. Seven fluoroacetamide-resistant mutants with low amidase activity gave rise to secondary mutant strains on succinate + butyra-mide plates. One appeared to be an up-promotor mutant and synthesized amidase at a high rate. This mutant, 433, was not stimulated by cyclic-AMP and was much less sensitive to catabolite repression by succinate. The mutation conferring resistance to catabolite repression was cotransduced at a frequency of 96 % (26/27) with the amidase genes . Five other revertants had catabolite repression-resistance mutations which were linked to the amidase genes and these also were probably promotor mutants. One strain had a mutation conferring resistance to catabolite repression which was unlinked to the amidase genes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-90-1-91
1975-09-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/90/1/mic-90-1-91.html?itemId=/content/journal/micro/10.1099/00221287-90-1-91&mimeType=html&fmt=ahah

References

  1. Betz J. L., Brown J. E., Clarke P. H., Day M. 1974; Genetic analysis of amidase mutants of Pseudomonas aeruginosa. Genetical Research 23:335–359
    [Google Scholar]
  2. Brammar W. J., Clarke P. H., Skinner A. J. 1967; Biochemical and genetic studies with regulator mutants of the Pseudomonas aeruginosa amidase system. Journal of General Microbiology 47:87–102
    [Google Scholar]
  3. Brown J. E., Brown P. R., Clarke P. H. 1969; Butyramide-utilizing mutants of Pseudomonas aeruginosa which produce an amidase with altered substrate specificity. Journal of General Microbiology 57:273–285
    [Google Scholar]
  4. Brown J. E., Clarke P. H. 1970; Mutations in a regulator gene allowing Pseudomonas aeruginosa 8602 to grow on butyramide. Journal of General Microbiology 64:329–342
    [Google Scholar]
  5. Clarke P. H., Tata R. 1973; Isolation of amidase-negative mutants of Pseudomonas aeruginosa by a positive selection method using an acetamide analogue. Journal of General Microbiology 75:231–234
    [Google Scholar]
  6. De Crombrugghe B., Chen B., Anderson W., Nissley P., Gottesman M., Pastan I., Perlman R. L. 1971; Lac DNA, RNA polymerase and cyclic AMP receptor protein, cyclic AMP, lac repressor and inducer are the essential elements for controlled lac transcription. Nature, New Biology 230:139–142
    [Google Scholar]
  7. Emmer M., De Crombrugghe B., Pastan I., Perlman R. 1970; Cyclic AMP receptor protein of E. coli: its role in the synthesis of inducible enzymes. Proceedings of the National Academy of Sciences of the United States of America 66:480–487
    [Google Scholar]
  8. Ippen K., Miller J. H., Scaife J., Beckwith J. 1968; New controlling element in the lac operon of E. coli. Nature; London: 217:825–827
    [Google Scholar]
  9. Potts J. R. 1975 Histidine catabolism in Pseudomonas aeruginosa. Ph.D. thesis University of London;
    [Google Scholar]
  10. Scaife J., Beckwith J. R. 1966; Mutational alteration of the maximal level of lac operon expression. Cold Spring Harbor Symposia on Quantitative Biology 31:403–408
    [Google Scholar]
  11. Silverstone A. E., Magasanik B., Reznikoff W. S., Miller J. H., Beckwith J. 1969; Catabolite sensitive site of the lac operon. Nature; London: 2211012–1014
    [Google Scholar]
  12. Smyth P. F., Clarke P. H. 1975; Catabolite repression of Pseudomonas aeruginosa amidase: the effect of carbon source on amidase synthesis. Journal of General Microbiology 90:81–90
    [Google Scholar]
  13. Zubay G., Schwartz D., Beckwith J. 1970; Mechanism of activation of catabolite-sensitive genes. A positive control system. Proceedings of the National Academy of Sciences of the United States of America 66:104–110
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-90-1-91
Loading
/content/journal/micro/10.1099/00221287-90-1-91
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error