1887

Abstract

SUMMARY: Poly(A)-containing RNA was isolated by cellulose column chromatography from total RNA extracted from var. 211/8p. RNA retained by the column was identified as poly(A)-containing RNA because it contained ribonuclease-resistant tracts, 25 to 55 nucleotides in length, from which not less than 80 % of base was found to be adenine after acid hydrolysis. The base composition of poly(A)-containing RNA differed from that of RNA (largely ribosomal) which did not adsorb to cellulose, having a higher adenine content and a lower guanine content. Poly(A)-containing RNA was polydisperse including molecules with mobilities from 10S to 40S with a mean of about 20S. In an system derived from wheat-germ, protein synthesis was stimulated by adding poly(A)-containing RNA from Chlorella. Optimum conditions were established in this system with respect to the amount of poly(A)-containing RNA added and the concentration of KC1 and Mg. It is proposed that, in Chlorella, poly(A)-containing RNA includes cytoplasmic mRNA as has been shown for some other eucaryotic organisms.

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/content/journal/micro/10.1099/00221287-89-1-155
1975-07-01
2021-05-06
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References

  1. Adesnik M., Darnell J.E. 1972; Biogenesis and characterisation of histone messenger RNA in HeLa cells. Journal of Molecular Biology 67:397–406
    [Google Scholar]
  2. Anderson J. F., Lewis J. B., Atkins J. F., Gesteland R. F. 1974; Cell-free synthesis of Adenovirus 2 proteins programmed by fractionated messenger RNA. A comparison of polypeptide products and messenger lengths. Proceedings of the National Academy of Sciences of the United States of America 71:2756–2760
    [Google Scholar]
  3. Bollum F. J. 1959; Thermal conversion of non-priming DNA to primer. Journal of Biological Chemistry 234:2733–2734
    [Google Scholar]
  4. Brawerman G. 1974; Eucaryotic messenger RNA. Annual Review of Biochemistry 43:621–642
    [Google Scholar]
  5. Burton K. 1969 In Data for Biochemical Research pp. 170–177 Dawson R. M. C. and others Edited by Oxford: Oxford University Press.;
    [Google Scholar]
  6. Darnell J. E., Jelinek W. R., Molloy G. R. 1973; Biogenesis of mRNA: genetic regulation in mammalian cells. Science; New York: 1811215–1221
    [Google Scholar]
  7. Eggitt M. J., Scragg A. H. 1975; The detection of RNA with messenger activity from Saccharomyces cerevisiae mitochondria. Proceedings of the Society for General Microbiology 2:47
    [Google Scholar]
  8. Ellis R. J. 1975; The search for plant messenger RNA. In Proceedings of the Fiftieth Anniversary Meeting of the Society for Experimental Biology in the Press
    [Google Scholar]
  9. Fouquet H., Bolme R., Wick R., Sauer H. W., Braun R. 1974; Isolation of adenylate-rich RNA from Physarum polycephalum. Biochimica et biophysica acta 353:313–322
    [Google Scholar]
  10. Groot G. S. P., Flavell R. A., Van Ommen G. J. B., Grivel L. A. 1974; Yeast mitochondrial RNA does not contain poly(A). Nature; London: 252167–169
    [Google Scholar]
  11. Higgins T. J. V., Mercer J. F. B., Goodwin P. B. 1973; Poly(A) sequences in plant polysomal RNA. Nature; New Biology 24668–70
    [Google Scholar]
  12. Hirsch M., Spradling A., Penman S. 1974; The messenger-like poly(A)-containing RNA species from the mitochondria of mammals and insects. Cell 1:31–35
    [Google Scholar]
  13. John P. C. L., Thurston C. F., Syrett P. J. 1970; Disappearance of isocitrate lyase enzyme from cells of Chlorella pyrenoidosa. Biochemical Journal 119:913–919
    [Google Scholar]
  14. Lodish H. F., Firtel R. A., Jacobson A. 1973; Transcription and structure of the genome of the cellular slime mould Dictyostelium discoideum. Cold Spring Harbor Symposia for Quantitative Biology 38:899–914
    [Google Scholar]
  15. Monjardino J. P. 1972; A simple method for base ratio analysis of small samples of RNA. Analytical Biochemistry 21:308–312
    [Google Scholar]
  16. Perlman S., Abelson H. T., Penman S. 1973; Mitochondrial protein synthesis: RNA with the properties of eucaryotic messenger RNA. Proceedings of the National Academy of Sciences of the United States of America 70:350–353
    [Google Scholar]
  17. Reed J., Wintersberger E. 1973; Adenylic acid-rich sequences in messenger RNA from yeast polysomes. FEBS Letters 32:213–217
    [Google Scholar]
  18. Roberts B. E., Paterson B. M. 1973; Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proceedings of the National Academy of Sciences of the United States of America 70:2330–2334
    [Google Scholar]
  19. Sagher D., Edelman M., Jakob K. M. 1974; Poly(A)-associated RNA in plants. Biochimica et biophysica acta 349:32–38
    [Google Scholar]
  20. Schutz G., Beato M., Feilgelson P. 1972; Isolation of eucaryotic messenger RNA on cellulose and its translation in vitro. Biochemical and Biophysical Research Communications 49:680–689
    [Google Scholar]
  21. Silver J. C., Horgen P.A. 1974; Hormonal regulation of presumptive mRNA in the fungus Achlya ambisexualis. Nature; London: 249252–254
    [Google Scholar]
  22. Van De Walle C. 1973; Polyadenylic sequences in plant RNA. FEBS Letters 34:31–34
    [Google Scholar]
  23. Weinberg R. A., Loening U., Willems M., Penman S. 1967; Acrylamide gel electrophoresis of HeLa cell nucleolar RNA. Proceedings of the National Academy of Sciences of the United States of America 58:1088–1095
    [Google Scholar]
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