1887

Abstract

SUMMARY: Large-scale synchronous cultures of were prepared by a sedimentationvelocity-size-selection method using a non-osmotic gradient in a zonal rotor. Synchrony indices of up to 0·68 were obtained (average length of cell-cycle, 5·1 h). Dry weight, protein and RNA increased continuously so as to double in one cell-cycle. Rates of oxygen uptake/ml culture increased overall so as to double over a cell-cycle, but rose to maxima at five periods in the cell-cycle. KCN gave a similar degree of inhibition throughout, and so did not alter the periodicity or amplitude of the oscillations. Total adenylates doubled over a cycle, and complex changes in pool sizes of ATP, ADP and AMP were temporally interrelated and were correlated with changes of oxygen uptake rates rather than with changes in biosynthetic requirements. Adenylate charge varied between 0·47 and 0·66. Discontinuous respiratory activity of mitochondria through the cell-cycle and possible mechanisms for its control are discussed with reference to previous data.

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1975-05-01
2021-10-25
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References

  1. Atkinson D. E., Walton G. M. 1967; Adenosine triphosphate conservation in metabolic regulation. Journal of Biological Chemistry 193:265–215
    [Google Scholar]
  2. Blumenthal L. K., Zahler S. A. 1962; Index for measurement of synchronization of cell populations. Science; New York: 135724
    [Google Scholar]
  3. Brooker B. E. 1971; Flagellar attachment and detachment of Crithidia fasciculata to the gut wall ofAnopheles gambiae. Protoplasma 73:191–202
    [Google Scholar]
  4. Campbell A. C. 1957; Synchronization of cell division. Bacteriological Reviews 21:263–272
    [Google Scholar]
  5. Chapman A. G., Fall L., Atkinson D. E. 1971; Adenylate energy charge in Escherichia coli during growth and starvation. Journal of Bacteriology 108:1072–1086
    [Google Scholar]
  6. Chin B., Bernstein I. A. 1968; Adenosine triphosphate and synchronous mitosis in Physarum polycephalum. Journal of Bacteriology 96:330–337
    [Google Scholar]
  7. Chiu S. V., Kao I. C, Erickson L. E., Fan L. T. 1973; ATP pools in activated sludge. Journal of Water Pollution Control Federation 45:1746–1758
    [Google Scholar]
  8. Cosgrove B., Skeen M. J. 1970; The cell-cycle in Crithidia fasciculata. Temporal relationships between synthesis of deoxyribonucleic acid in nucleus and in the kinetoplast. Journal of Protozoology 17: 172–177
    [Google Scholar]
  9. Edwards C., Lloyd D. 1973; Terminal oxidases and carbon monoxide-reacting haemoproteins in the trypanosomatid, Crithidia fasciculata. Journal of General Microbiology 79:275–284
    [Google Scholar]
  10. Epel D. 1963; The effects of carbon monoxide inhibition on ATP level and the rate of mitosis in the sea urchin egg. Journal of Cell Biology 17:315–319
    [Google Scholar]
  11. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells. In Methods in Microbiology 5B pp. 209–344 Norris J. R., Ribbons D. W. London and New York: Academic Press;
    [Google Scholar]
  12. Lloyd D. 1974 Mitochondria of Microorganisms London, New York: Academic Press;
    [Google Scholar]
  13. Lloyd D., Brookman J. S. G. 1967; An oxygen electrode reaction vessel. Biotechnology and Bioengineering 9:271–272
    [Google Scholar]
  14. Lloyd D., John L., Edwards C., Chagla A. H. 1975; Synchronous cultures of micro-organisms: large-scale preparation by continuous-flow size selection. Journal of General Microbiology 88:153–158
    [Google Scholar]
  15. Mitchison J. M. 1971 The Biology of the Cell Cycle Cambridge University Press:
    [Google Scholar]
  16. Mitchison J. M., Vincent W. S. 1965; Preparation of synchronous cell cultures by sedimentation. Nature: London; 205987–989
    [Google Scholar]
  17. Newton B. A. 1957; The mode of action of phenanthridines: the effect of ethidium bromide on cell division and nucleic acid synthesis. Journal of General Microbiology 17:718–730
    [Google Scholar]
  18. Poole R. K., Lloyd D. 1974; Changes in respiratory activities during the cell-cycle of the fission yeast, Schizosaccharomyces pombe 972h, growing in the presence of glycerol. Biochemical Journal 144:141–148
    [Google Scholar]
  19. Poole R. K., Lloyd D., Kemp R. B. 1973; Respiratory oscillations and heat evolution in synchronously dividing cultures of the fission yeast, Schizosaccharomyces pombe 972h. Journal of General Microbiology 77:209–220
    [Google Scholar]
  20. Sachsenmaier W., Scholz R., Grunet J. 1968; Änderungen des Nucleotid-Spiegels im Verlauf des synchronen Mitosezyklus vonPhysarum polycephalum. Hoppe-Seyler’s Zeitschrift für physiologische Chemie 349:1257
    [Google Scholar]
  21. Simpson L. 1972; The kinetoplast of the Hemoflagellates. International Review of Cytology 32:139–207
    [Google Scholar]
  22. Simpson L., Braly P. 1970; Synchronization of Leishmania tarentolae by hydroxyurea. Journal of Protozoology 17:511–517
    [Google Scholar]
  23. Steinert M. 1969; Reversible inhibition of the division of Crithidia luciliae by hydroxyurea and its use for obtaining synchronized cultures. FEBS Letters 5:291–294
    [Google Scholar]
  24. Steinert M., Steinert G. 1962; La synthèse de l’acide désoxyribonucléique au cours du cycle de division de Trypanosoma mega. Journal of Protozoology 9:203–211
    [Google Scholar]
  25. Steinert M., Van Assel S. 1967; Replications coordonées des acides désoxyribonucléiques nucléaire et mitochondrial chez Crithidia luciliae. Archives internationales de physiologie et de biochimie 75:370–371
    [Google Scholar]
  26. Stuart K., Hanson E. D. 1967; Acriflavin induction of dyskinetoplasy in Leptomonas karyophilus. Journal of Protozoology 14:39–43
    [Google Scholar]
  27. Van Assel S., Steinert G. 1971; Nuclear and kinetoplastic DNA replication cycles in normal and synchronously dividing Crithidia luciliae. Experimental Cell Research 65:353–358
    [Google Scholar]
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