1887

Abstract

SUMMARY: A total of 41 mutants lacking NADP -glutamate dehydrogenase (NADP-GDH) activity have been studied. All the mutations were located at the locus within 0·1% recombination of Two mutants, and out of five examined, produced cross-reacting material which neutralized NADP-GDH antiserum. The mutant has altered values for all five substrates: ammonium, -ketoglutarate, -glutamate, NADPH and NADP. The mutant had temperature-sensitive growth, abnormal ammonium-regulation characteristics and thermolabile NADP-GDH activity. These results show that is the structural gene for NADP-GDH.

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/content/journal/micro/10.1099/00221287-86-2-294
1975-02-01
2024-12-12
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References

  1. Adelberg E. A., Mandel M., Chen G. C. C. 1965; Optimum conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli K12. Biochemical and Biophysical Research Communications 18:788–795
    [Google Scholar]
  2. Arst H. N., Macdonald D. W. 1973; A mutant of Aspergillus nidulans lacking NADP-linked glutamate dehydrogenase. Molecular and General Genetics 122:261–265
    [Google Scholar]
  3. Cohen B. L. 1972; Ammonium repression of extracellular protease in Aspergillus nidulans. Journal of General Microbiology 71:293–299
    [Google Scholar]
  4. Cohen B. L. 1973; Regulation of intracellular and extracellular neutral and alkaline proteases in Aspergillus nidulans. Journal of General Microbiology 79:311–320
    [Google Scholar]
  5. Dunn E., Pateman J. A. 1972; Urea and thiourea uptake in Aspergillus nidulans. Heredity 29:129
    [Google Scholar]
  6. Fincham J. R. S., Bond P. A. 1960; A further genetic variety of glutamic acid dehydrogenase in Neurospora crassa. Biochemical Journal 77:96–105
    [Google Scholar]
  7. Fincham J. R. S., Stadler D. R. 1965; Complementation relationships of Neurospora am mutants in relation to their formation of abnormal varieties of glutamate dehydrogenase. Genetical Research 6:121–129
    [Google Scholar]
  8. Hynes M. J., Pateman J. A. 1970a; The genetic analysis of regulation of amidase synthesis in Aspergillus nidulans. I. Mutants unable to utilize acrylamide. Molecular and General Genetics 108:97–106
    [Google Scholar]
  9. Hynes M. J., Pateman J. A. 1970b; The genetic analysis of regulation of amidase synthesis in Aspergillus nidulans. II. Mutants resistant to fluoroacetamide. Molecular and General Genetics 118:107–116
    [Google Scholar]
  10. Kinghorn J. R., Pateman J. A. 1972; Regulation of glutamate transport in Aspergillus nidulans. Heredity 29:128
    [Google Scholar]
  11. Kinghorn J. R., Pateman J. A. 1973a; NAD and NADP l-glutamate dehydrogenase and ammonium regulation in Aspergillus nidulans. Journal of General Microbiology 78:39–46
    [Google Scholar]
  12. Kinghorn J. R., Pateman J. A. 1973b; A new class of mutants affecting ammonium regulation and methylammonium resistance in Aspergillus nidulans. Heredity 31:427
    [Google Scholar]
  13. Markert C. L., Moller F. 1959; Multiple forms of enzymes: tissue ontogenetic and other specific patterns. Proceedings of the National Academy of Sciences of the United States of America 45753–759
    [Google Scholar]
  14. Pateman J. A., Cove D. J. 1967; Regulation of nitrate reduction in Aspergillus nidulans. Nature; London: 2151234–1239
    [Google Scholar]
  15. Pateman J. A., Dunn E., Kinghorn J. R., Forbes E. 1974; Ammonium and methylammonium transport in wild type and mutant cells of Aspergillus nidulans. Molecular and General Genetics 133:225–236
    [Google Scholar]
  16. Pateman J. A., Fincham J. R. S. 1964; Complementation and enzyme studies of revertants induced in an am mutant of Neurospora crassa. Genetical Research 6:419–432
    [Google Scholar]
  17. Pateman J. A., Kinghorn J. R., Dunn E. 1974; Regulatory aspects of l-glutamate transport in Aspergillus nidulans. Journal of Bacteriology 119:534–542
    [Google Scholar]
  18. Pateman J. A., Kinghorn J. R., Dunn E., Forbes E. 1973; Ammonium regulation in Aspergillus nidulans. Journal of Bacteriology 114:943–950
    [Google Scholar]
  19. Pontecorvo G., Roper J. A., Hemmons L. M., Macdonald K. D., Bufton A. W. J. 1953; The genetics of Aspergillus nidulans. Advances in Genetics 5:141–238
    [Google Scholar]
  20. Poulik M. D. 1957; Starch gel electrophoresis in a discontinuous system of buffers. Nature; London: 1801477–1479
    [Google Scholar]
  21. Scazzocchio C., Darlington A. J. 1968; The induction and repression of the enzymes of purine breakdown in Aspergillus nidulans. Biochimica et biophysica acta 166:557–568
    [Google Scholar]
  22. Smithies O. 1955; Zone electrophoresis in starch gells: group variations in the serum protein of normal human adults. Biochemical Journal 61:629–635
    [Google Scholar]
  23. Stachow C. S., Sanwal B. D. 1967; Regulation, purification and some properties of the NAD-specific glutamate dehydrogenases of Neurospora. Biochimica et biophysica acta 139:294–307
    [Google Scholar]
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