1887

Abstract

SUMMARY: A total of 41 mutants lacking NADP -glutamate dehydrogenase (NADP-GDH) activity have been studied. All the mutations were located at the locus within 0·1% recombination of Two mutants, and out of five examined, produced cross-reacting material which neutralized NADP-GDH antiserum. The mutant has altered values for all five substrates: ammonium, -ketoglutarate, -glutamate, NADPH and NADP. The mutant had temperature-sensitive growth, abnormal ammonium-regulation characteristics and thermolabile NADP-GDH activity. These results show that is the structural gene for NADP-GDH.

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/content/journal/micro/10.1099/00221287-86-2-294
1975-02-01
2021-07-28
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