SUMMARY: wvu strain 3672 accumulates storage polysaccharides when grown in a glucose-supplemented medium containing a growth-limiting concentration of nitrogen. Structural analysis of an isolated and purified sample identified the material as a glycogen-like polysaccharide possessing an average chain length of 11.6 glucosyl units and iodine absorption maximum at 460 nm. Cytochemical staining of thin sections of whole organisms revealed granules ranging in size from 60 to 100 nm in diameter distributed throughout the cytoplasm. Glycogen granules could easily be differentiated from lipid storage vacuoles after treatment of thin sections with a glycogen-specific staining procedure. Negatively stained preparations of glycogen granules isolated with cold water revealed α-particles composed of indistinct sub-units.


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