Results from experiments in strongly suggest that major roles can be ascribed to polyamines in controlling the stability, activity and synthesis of ribonucleic acids. Furthermore, functional substitution of polyamines for inorganic cations, particularly magnesium ions, in cell-free protein synthesis is well substantiated (see Cohen, 1971). Recently we have been analysing the effects of culture conditions on the chemical composition of and have found fluctuations in polyamine and magnesium concentrations in response to a changing environment, while biomass and RNA remained constant. This paper describes the influence of steady-state growth rate on hyphal concentrations of spermidine, spermine and Mg ions.


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