1887

Abstract

SUMMARY

The octadecenoic acid growth-requirement for () a can be effectively replaced by -9,10-methylenehexadecanoic acid but not by m-9,10-hexadecenoic acid. Once incorporated into the membrane polar lipids, this acid is also elongated to -11,12-methyleneoctadecanoic acid. This is the first instance of the elongation of a cyclopropane fatty acid by a microorganism. -9,10-Methylenehexadecanoic acid, like -9,10-octadecenoic acid, alters the osmotic fragility of this mycoplasma, but while a correlation exists between fatty acid concentration and yield of organism, there is no apparent relationship between fatty acid concentration, maximal yield and greatest membrane osmotic stability for this organism. The fatty acid composition of the membrane polar lipids of a grown with -11,12-octadecenoic and -9,10-methylenehexadecanoic acids shows each acid to be incorporated to a similar extent. A cell-free system from a synthesized less cw-11,12- methyleneoctadecanoic and tetradecanoic acids but more octadecanoic acid when compared with the fatty acid composition of the intact organism.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-80-1-93
1974-01-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/80/1/mic-80-1-93.html?itemId=/content/journal/micro/10.1099/00221287-80-1-93&mimeType=html&fmt=ahah

References

  1. Brian B. L., Gracy R. W., Scholes V. E. 1972; Gas chromatography of cyclopropane fatty acid methylesters prepared with methanolic boron trichloride and boron trifluoride. Journal of Chromatography 66:138–140
    [Google Scholar]
  2. Cohen M., Panos C. 1966; Membrane lipid composition of Streptococcus pyogenes and derived l-form. Biochemistry 5:2385–2392
    [Google Scholar]
  3. Dawidowicz E. A., Thompson T. E. 1971; Artifacts produced by boron trifluoridemethanolysis of a synthetic lecithin containing cyclopropane fatty acids (1-2-dihydrosterculosyl-3-sn-phosphatidylcholine). Journal of Lipid Research 12:636–637
    [Google Scholar]
  4. Dittmer J. C., Lester R. L. 1964; A simple, specific spray for the detection of phospholipids on thin-layer chromatograms. Journal of Lipid Research 5:126–127
    [Google Scholar]
  5. Goldfine H., Panos C. 1971; Phospholipids of Clostridium butyricum. IV. Analysis of the positional isomers of monounsaturated and cyclopropane fatty acids and alk-Í-enyl ethers by capillary column chromatography. Journal of Lipid Research 12:214–220
    [Google Scholar]
  6. Henrikson C. V., Panos C. 1969; Fatty acid composition, distribution, and requirements of two nonsterol-requiring mycoplasmas from complex but defatted growth media. Biochemistry 8:646–651
    [Google Scholar]
  7. Hofmann K. 1962 Fatty Acid Metabolism in Microorganisms New York: J. Wiley;
    [Google Scholar]
  8. Hofmann K., Henis D. B., Panos C. 1957; Fatty acid interconversions in lactobacilli. Journal of Biological Chemistry 228:349–355
    [Google Scholar]
  9. Hofmann K., Lucas R. A., Sax S. M. 1952; The chemical nature of the fatty acids of Lactobacillus arabinosus. Journal of Biological Chemistry 195:473–485
    [Google Scholar]
  10. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  11. O’Leary W. M. 1967 The Chemistry and Metabolism of Microbial Lipids Cleveland: World Publishing;
    [Google Scholar]
  12. Panos C., Cohen M., Fagan G. 1966; Lipid alterations after cell wall inhibition. Fatty acid content of Streptococcus pyogenes and derived L-form. Biochemistry 5:1461–1468
    [Google Scholar]
  13. Panos C., Henrikson C. V. 1968; Resolution of positional isomers of bacterial long chain cyclopropanering containing fatty acids by capillary column chromatography. Journal of Gas Chromatography 6:551–554
    [Google Scholar]
  14. Panos C., Henrikson C. V. 1969; Fatty acid interconversions in Mycoplasma sp. khs. Biochemistry 8:652–658
    [Google Scholar]
  15. Panos C., Rottem S. 1970; Incorporation and elongation of fatty acid isomers by Mycoplasma laidlawii A. Biochemistry 9:407–412
    [Google Scholar]
  16. Razin S. 1973 Physiology of Mycoplasmas. Advances of Microbial Physiology 9: in the Press
    [Google Scholar]
  17. Razin S., Tourtellotte M. E., McElhaney R. N., Pollack J. D. 1966; Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells. Journal of Bacteriology 91:609–616
    [Google Scholar]
  18. Rottem S., Panos C. 1969; The effect of long chain fatty acid isomers on growth, fatty acid composition and osmotic fragility of Mycoplasma laidlawii A. Journal of General Microbiology 59:317–328
    [Google Scholar]
  19. Rottem S., Panos C. 1970; The synthesis of long-chain fatty acids by a cell-free system from Mycoplasma laidlawii A. Biochemistry 9:57–63
    [Google Scholar]
  20. Smith P. F. 1971 The Biology of Mycoplasmas New York: Academic Press;
    [Google Scholar]
  21. Weeks G., Shapiro M., Burns R. O., Wakil S. J. 1969; Control of fatty acid metabolism. I. Induction of the enzymes of fatty acid oxidation in Escherichia coli. Journal of Bacteriology 97:827–836
    [Google Scholar]
  22. Weinbaum G., Panos C. 1966; Fatty acid distribution in normal and filamentous Escherichia coli. Journal of Bacteriology 92:1576–1577
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-80-1-93
Loading
/content/journal/micro/10.1099/00221287-80-1-93
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error