1887

Abstract

SUMMARY: The sensitivity of to sphaeroplast formation has been examined during the transition from the exponential phase of growth to the stationary phase. Exponential-phase yeasts are sensitive to sphaeroplast formation while stationary yeasts are resistant. During the transition period, there is a rapid increase in resistance to sphaeroplast formation. This increase can be inhibited by treatment with either cycloheximide or 5-fluorouracil. It is suggested that the resistance to sphaeroplast formation characteristic of stationary-phase yeasts is the result of a specific modification of the yeasts during the transition period which is dependent on both RNA and protein synthesis.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-80-1-259
1974-01-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/micro/80/1/mic-80-1-259.html?itemId=/content/journal/micro/10.1099/00221287-80-1-259&mimeType=html&fmt=ahah

References

  1. Anderson F. B., Millbank J. W. 1966; Protoplast formation and yeast cell wall structure. The action of the enzymes of the snail Helix pomatia . Biochemical Journal 99:682–687
    [Google Scholar]
  2. Bevan E. A., Costello W. P. 1964; The preparation and use of an enzyme which breaks open yeast asci. Microbial Genetics Bulletin 21:5
    [Google Scholar]
  3. Blamire J., Cryer D. R., Finkelstein D. B., Marmur J. 1972; Sedimentation properties of yeast nuclear and mitochondrial DNA. Journal of Molecular Biology 67:11–24
    [Google Scholar]
  4. Brown J. P. 1971; Sensitivity of the cell walls of some yeasts to lysis by enzymes of Helix pomatia . Canadian Journal of Microbiology 17:205–208
    [Google Scholar]
  5. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62:315–323
    [Google Scholar]
  6. Cox B. S., Bevan E. A. 1962; Aneuploidy in yeast. New Phytologist 61:342–355
    [Google Scholar]
  7. Darling S., Theilade J., Birch-Anderson A. 1969; Kinetic and morphological observations on Saccharomyces cerevisiae during spheroplast formation. Journal of Bacteriology 98:797–810
    [Google Scholar]
  8. Duell E. A., Inoue S., Utter M. F. 1964; Isolation and properties of intact mitochondria from spheroplasts of yeast. Journal of Bacteriology 88:1762–1773
    [Google Scholar]
  9. Eddy A. A., Williamson D. H. 1957; A method of isolating protoplasts from yeast. Nature; London: 1791252–1253
    [Google Scholar]
  10. Garcia Mendoza C., Villanueva J. R. 1962; Production of yeast protoplasts by an enzyme preparation of Streptomyces sp. Nature; London: 1951326–1327
    [Google Scholar]
  11. Hutchison H. T., Hartwell L. H. 1967; Macromolecule synthesis in yeast spheroplasts. Journal of Bacteriology 94:1697–1705
    [Google Scholar]
  12. Kerridge D. 1958; The effect of actidione and other anti-fungal agents on nucleic acid and PROTEIN synthesis in Saccharomyces carlsbergensis . Journal of General Microbiology 19:467–506
    [Google Scholar]
  13. de Kloet S. R. 1968; Effects of 5-fluorouracil and 6-azauracil on the synthesis of ribonucleic acid and protein in Saccharomyces carlsbergensis . Biochemical Journal 106:167–178
    [Google Scholar]
  14. Kôvac L., Bednarova H., Greksak M. 1968; Oxidative phosphorylation in yeast. I. Isolation and properties of phosphorylating mitochondria from stationary phase cells. Biochimica et biophysica acta 153:32–42
    [Google Scholar]
  15. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  16. Mann J. W., Heintz C. E., Macmillan J. D. 1972; Yeast spheroplasts formed by cell wall-degrading enzymes from Oerskovia sp. Journal of Bacteriology 111:821–824
    [Google Scholar]
  17. Nečas O. 1971; Cell wall synthesis in yeast protoplasts. Bacteriological Reviews 35:149–170
    [Google Scholar]
  18. Nickerson W. J., Falcone G. 1956; Enzymatic reduction of disulfide bonds in cell wall protein of bakers’ yeast. Science; New York: 124318–319
    [Google Scholar]
  19. Northcote D. H., Horne R. W. 1952; The chemical composition and structure of the yeast cell wall. Biochemical Journal 51:232–236
    [Google Scholar]
  20. Parry E. M., Cox B. S. 1972; The general properties of a strain of yeast with less DNA than normal. Journal of General Microbiology 70:129–149
    [Google Scholar]
  21. Petes T. D., Fangman W. L. 1972; Sedimentation properties of yeast chromosomal DNA. Proceedings of the National Academy of Sciences of the United States of America 69:1188–1191
    [Google Scholar]
  22. Rost K., Venner H. 1965; Enzymatische Zellwand verdauung bei Hefen durch Schneckenenzym. Archiv für Mikrobiologie 51:122–129
    [Google Scholar]
  23. Schneider W. C. 1957; Determination of nucleic acids in tissues by pentose analysis. In Methods in Enzymology 3 pp. 680–684 Colowick S. P., Kaplan N. O. Edited by New York: Academic Press;
    [Google Scholar]
  24. Shahin M. M. 1972; Relationship between yield of protoplasts and growth phase in Saccharomyces . Journal of Bacteriology 110:767–771
    [Google Scholar]
  25. Shapiro L., Grossman L. I., Marmur J., Kleinschmidt A. K. 1968; Physical studies on the structure of yeast mitochondrial DNA. Journal of Molecular Biology 33:907–922
    [Google Scholar]
  26. Sommers A., Lewis M. J. 1971; Effect of dithiothreitol on yeast: spheroplast formation and invertase release. Journal of General Microbiology 69:327–335
    [Google Scholar]
  27. Wintersberger U., Smith P., Letnansky K. 1973; Yeast chromatin: preparation from isolated nuclei, histone composition and transcription capacity. European Journal of Biochemistry 33:112–130
    [Google Scholar]
/content/journal/micro/10.1099/00221287-80-1-259
Loading
/content/journal/micro/10.1099/00221287-80-1-259
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error