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Abstract
SUMMARY: The germination rate of Clostridium bifermentans spores and the manner in which it is influenced by pH, sodium ion concentration and temperature differ according to the organic germinant used. Only l-alanine initiated germination when present alone. The germination rate with optimal l-alanine was increased by l-phenylalanine, l-arginine, l-histidine or l-lysine but not by other common amino acids or l-lactate. With suboptimal concentrations of l-alanine, addition of l-lactate, l-arginine or l-phenylalanine increased the germination rate more than an equal concentration of l-alanine. The mechanisms whereby l-phenylalanine and l-arginine stimulate germination appear to be different from those involved when l-alanine or l-lactate are the germinants.
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