1887

Abstract

SUMMARY: The regulation of the first step in methionine biosynthesis, homoserine--transsuccinylase, has been examined in a methionine-requiring mutant of the blue-green alga . Using an improved assay for homoserine--transsuccinylase, no evidence of derepression of the biosynthesis of this enzyme was found, even under conditions of acute methionine starvation. End-product inhibition of the enzyme by homoserine, cystathionine and methionine was demonstrated, and shown in the latter case to be of an allosteric nature. The lack of transcription control of this enzyme is discussed as an example of a general phenomenon in this group of micro-organisms.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-79-1-89
1973-11-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/79/1/mic-79-1-89.html?itemId=/content/journal/micro/10.1099/00221287-79-1-89&mimeType=html&fmt=ahah

References

  1. Allen M. M. 1968; Simple conditions for growth of unicellular blue-green algae on plates. Journal of Physiology 4:1–4
    [Google Scholar]
  2. Carr N. G. 1973a; Metabolic control and autotrophic physiology. In The Biology of Blue-Green Algae Edited by Carr N. G., Whitton B. A. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  3. Carr N. G. 1973b; Mechanism of autotrophic physiology in the blue-green algae. Journal of General Microbiology 75:v
    [Google Scholar]
  4. Herdman M., Carr N. G. 1972; The isolation and characterization of mutant strains of the blue-green alga Anacystis nidulans. Journal of General Microbiology 70:213–220
    [Google Scholar]
  5. Holden J., Morris I. 1970; Regulation of arginine biosynthesis in Chlamydomonas reinhardii: studies in vivo and of ornithine transcarbamylase and argininosuccinate lyase activities. Archiv für Mikrobiologie 74:58–68
    [Google Scholar]
  6. Hood W., Carr N. G. 1971; Apparent lack of control by repression of arginine metabolism in blue-green algae. Journal of Bacteriology 107:365–367
    [Google Scholar]
  7. Hood W., Carr N. G. 1972; Branched chain amino acid biosynthesis in the blue-green algae. Journal of General Microbiology 73:417–426
    [Google Scholar]
  8. Kratz W. A., Myers J. 1955; Nutrition and growth of several blue green algae. American Journal of Botany 42:282–287
    [Google Scholar]
  9. Lawrence D. A. 1972; Regulation of the methionine feed back sensitive enzyme in mutants of Salmonella typhimurium. Journal of Bacteriology 109:8–11
    [Google Scholar]
  10. Rowbury R. J. 1964; Synthesis of cystathionine and its control in Salmonella typhimurium. Nature, London 203:977–978
    [Google Scholar]
  11. Weber H. L., Böck A. 1968; Comparative studies on the regulation of DAHP synthetase activity in blue-green and green algae. Archiv für Mikrobiologie 61:159–168
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-79-1-89
Loading
/content/journal/micro/10.1099/00221287-79-1-89
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error