1887

Abstract

Summary: Axenic cultures of f. sp. cannot reduce sulphate for sulphur-amino-acid synthesis. Although [S]sulphide is incorporated into these amino acids, the fungus still has a nutritional requirement for a sulphur amino acid. Most of the incorporated sulphide was found in the culture filtrate in the form of cysteine, -methylcysteine, glutathione and cysteinylglycine. There were appreciable amounts of labelled methionine in mycelial protein but very little of this amino acid appeared in the culture filtrate. The results indicate that the nutritional requirements for sulphur amino acids by the rust fungus is caused by loss of cysteine and some of its derivatives into the medium.

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/content/journal/micro/10.1099/00221287-76-2-345
1973-06-01
2021-08-05
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References

  1. Barner H. D., Cantino E. C. 1952; Nutritional relationships in a new species of Blastocladietla . American Journal of Botany 39:746–751
    [Google Scholar]
  2. Benko P. V., Wood T. C., Segel I. H. 1967; Specificity and regulation of methionine transport in the filamentous fungi. Archives of Biochemistry and Biophysics 122:738–804
    [Google Scholar]
  3. Bray G. A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analytical Biochemistry 1:279–285
    [Google Scholar]
  4. Cantino E. C. 1950; Nutrition and Phylogeny in the water molds. Quarterly Review of Biology 25:269–277
    [Google Scholar]
  5. Cantino E. C. 1955; Physiology and Phylogeny in the water molds—a re-evaluation. Quarterly Review of Biology 30:138–149
    [Google Scholar]
  6. Daly J. M., Knoche H. W., Wiese M. V. 1967; Carbohydrate and lipid metabolism during germination of uredospores of Puccinia graminis tritici . Plant Physiology 42:1633–1641
    [Google Scholar]
  7. Dekker J. 1969; l-Methionine induced inhibition of powdery mildew and its reversal by folic acid. Netherlands Journal of Plant Pathology 75:182–185
    [Google Scholar]
  8. Delavier-Klutchko C., Flavin M. 1965; Enzymic synthesis and cleavage of cystathionine in fungi and bacteria. Journal of Biological Chemistry 240:2537–2549
    [Google Scholar]
  9. Grabow W. O. K., Smit J. A. 1967; Methionine synthesis in Proteus mirabilis . Journal of General Microbiology 46:47–57
    [Google Scholar]
  10. Hanes C. S., Hird F. J. R., Isherwood F. A. 1950; Synthesis of peptides in enzymic reactions involving glutathione. Nature, London 166:288–292
    [Google Scholar]
  11. Heilmann J., Barrollier J., Watzke E. 1957; Beitrag zur Aminosäurebestimmung auf Papier-chromatogrammen. Zeitschrift für physiologische Chemie 309:219–220
    [Google Scholar]
  12. Howes N. K., Scott K. J. 1972; Sulphur nutrition of Puccinia graminis f sp. tritici in axenic culture. Canadian Journal of Botany 50:1165–1170
    [Google Scholar]
  13. Ingraham J. L., Emerson R. 1954; Studies of the nutrition and metabolism of the aquatic phycomycete, Allomyces . American Journal of Botany 41:146–152
    [Google Scholar]
  14. Jackson A. O., Samborski D. J., Rohringer R., Kim W. K. 1970; Folate derivatives in ungerminated and germinated uredospores of wheat stem rust. Canadian Journal of Botany 48:1617–1623
    [Google Scholar]
  15. Jones J. P., Snow J. P. 1965; Amino acids released during germination of S35 labelled crown rust spores. Phytopathology (Abstr.) 55:499
    [Google Scholar]
  16. Kanazawa A., Katimoto Y., Nakajima T., Sano I. 1965; Identification of γ-glutamylserine, γ-gluta-mylalanine, γ-glutamylvaline and S-methylglutathione of bovine brain. Biochimica et biophysica acta 111:90–95
    [Google Scholar]
  17. Katz S., Lewis A. 1966; Apparatus for high-voltage thin layer electrophoresis. Application to amino acid analysis. Analytical Biochemistry 17:300–309
    [Google Scholar]
  18. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  19. Maclean D. J., Scott K. J. 1970; Variant forms of saprophytic mycelium grown from uredospores of Puccinia graminis f sp. tritici . Journal of General Microbiology 64:19–27
    [Google Scholar]
  20. Maclean D. J., Scott K. J., Tommerup I. C. 1971; A uninucleate wheat-infecting strain of the stem rust fungus isolated from axenic cultures. Journal of General Microbiology 65:339–342
    [Google Scholar]
  21. Morris C. J., Thompson J. F. 1956; The identification of ( + ) S-methyl-l-cysteine sulfoxide in plants. Journal of the American Chemical Society 78:1605–1608
    [Google Scholar]
  22. Morton A. G., Broadbent D. 1955; The formation of extra-cellular nitrogen compounds by fungi. Journal of General Microbiology 12:248–258
    [Google Scholar]
  23. Orlowski M., Meister A. 1970; The γ-glutamyl cycle: A possible transport system for amino acids. Proceedings of the National Academy of Science of the United States of America 67:1248–1255
    [Google Scholar]
  24. Ragland J. B., Liverman J. L. 1956; S-Methyl-l-cysteine as a naturally occurring metabolite in Neurospora crassa. Archives of Biochemistry and Biophysics 65:574–576
    [Google Scholar]
  25. Ryan F. J., Beadle G. W., Tatum E. L. 1943; The tube method of measuring the growth rate of Neurospora . American Journal of Botany 30:784–799
    [Google Scholar]
  26. Samborski D. J., Forsyth F. R. 1960; Inhibition of rust development of detached wheat leaves by metabolites, antimetabolites, and enzyme poisons. Canadian Journal of Botany 38:467–476
    [Google Scholar]
  27. Schmidt G., Thannhauser S. L. 1945; A method for the determination of deoxyribonucleic acid, ribonucleic acid and phosphoproteins in animal tissue. Journal of Biological Chemistry 161:83–89
    [Google Scholar]
  28. Thompson J. F. 1967; Sulphur metabolism in plants. Annual Review of Plant Physiology 18:59–84
    [Google Scholar]
  29. Tkachuk R. 1970; l-Cysteinylglycine: its occurrence and identification. Canadian Journal of Biochemistry 48:1029–1036
    [Google Scholar]
  30. Waelisch H. 1952; Certain aspects of intermediary metabolism of glutamine, asparagine and glutathione. Advances in Enzymology 13:237–319
    [Google Scholar]
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