1887

Abstract

Summary: Strains of derived from a virus-infected culture have been investigated for reduction in virus titre. These strains were obtained following treatment with either heat (74°C) or specific antimetabolites. Virus titres were measured with a combination of immunological and radiochemical assays. One strain was cured of the mycophage and exhibited an increased growth rate and stability. Other strains, which show an apparent reduction in intracellular virus titre, actually released increased levels of virus from cells. Lytic plaques developed when infected strains were grown on an unbuffered, high lactose-containing medium, and virus titres could be correlated with plaque formation. All strains, regardless of virus titre, retained the ability to synthesize penicillin.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-76-2-265
1973-06-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/76/2/mic-76-2-265.html?itemId=/content/journal/micro/10.1099/00221287-76-2-265&mimeType=html&fmt=ahah

References

  1. Backus M. P., Stauffer J. F. 1955; The production and selection of a family of strains in Penicillium chrysogenum . Mycologia 47:429–463
    [Google Scholar]
  2. Banks G. T., Buck K. W., Chain E. B., Darbyshire J. E., Himmelweit F. 1969; Virus-like particles in penicillin producing strains of Penicillium chrysogenum . Nature, London 222:89–90
    [Google Scholar]
  3. Banks G. T., Buck K. W., Chain E. B., Himmelweit F., Marks J. E., Tyler J. M., Hollings M., Last F. T., Stone O. M. 1968; Viruses in fungi and interferon stimulation. Nature, London 218:542–545
    [Google Scholar]
  4. Borre E., Morgantini L. E., Ortali V., Tonolo A. 1971; Production of lytic plaques of viral origin in Penicillium . Nature, London 229:568–569
    [Google Scholar]
  5. Boxer G. E., Everett P. M. 1949; Colorimetric determination of benzyl-penicillin. Analytical Chemistry 21:670–673
    [Google Scholar]
  6. Bozarth R. F., Wood H. A., Mandelbrot A. 1971; The Penicillium stoloniferum virus complex. Two double-stranded RNA virus-like particles in a single cell. Virology 45:516–523
    [Google Scholar]
  7. Buck K. W., Chain E. B., Darbyshire J. E. 1969; High cell wall galactosamine content and virus particles in Penicillium stoloniferum . Nature, London 223:1273
    [Google Scholar]
  8. Buck K. W., Chain E. B., Himmelweit F. 1971; Comparison of interferon induction in mice by purified Penicillium chrysogenum virus and derived double-stranded RNA. Journal of General Virology 12:131–139
    [Google Scholar]
  9. Buck K. W., Kempson-Jones G. F. 1970; Three types of virus particle in Penicillium stoloniferum . Nature, London 225:945–946
    [Google Scholar]
  10. Caten C. E. 1972; Vegetative incompatibility and cytoplasmic infection in fungi. Journal of Genera Microbiology 72:221–229
    [Google Scholar]
  11. Day L. E., Ellis L. F. 1971; Virus-like particles in Cephalosporium acremonium . Applied Microbiology 22:919–920
    [Google Scholar]
  12. Elander R. P. 1967; Enhanced penicillin biosynthesis in mutant and recombinant strains of Penicillium chrysogenum . In Induced Mutations and Their Utilization pp 403–423 Abhandlungen der Deutschen Akademie der Wissenschaften zu Berlin;
    [Google Scholar]
  13. Hollings M., Stone O. M. 1971; Viruses that infect fungi. Annual Review of Phytopathology 9:93–118
    [Google Scholar]
  14. Horwitz M. S., Scharff M. D. 1969; Immunological precipitation of radioactively labelled viral proteins. In Fundamental Techniques in Virology Edited by Habel K., Salzman N. P. New York: Academic Press;
    [Google Scholar]
  15. Jinks J. L. 1959; Lethal suppression cytoplasms in aged clones of Aspergillus glaucus . Journal of General Microbiology 21:397–409
    [Google Scholar]
  16. Kazama F. Y., Schornstein K. L. 1972; Herpes-type virus particles associated with a fungus. Science, New York 177:696–697
    [Google Scholar]
  17. Koltin Y., Berick R., Stamberg J., Ben-Shaul Y. 1973; Virus-like particles and cytoplasmic inheritance of plaques in a higher fungus. Nature New Biology 241:108–109
    [Google Scholar]
  18. Lapierre H., Albouy J., Faivre-Amiot A., Molin G. 1971; Mise en évidence de particules virales dans divers champignons du genre Sclerotium . Comptes rendus de l’Academie des sciences (Ser D.) 272:2848–2851
    [Google Scholar]
  19. Lapierre H., Astier-Manifacier S., Cornuet P. 1971; Activité RNA polymérase associée aux préparations purificés de virus du Penicillium stoloniferum . Comptes rendus de lAcadémie des sciences (Ser D.) 273:992–994
    [Google Scholar]
  20. Lapierre H., Faivre-Amiot A., Kusiak C., Molin G. 1972; Particules de type viral associées au Mycogone perniciosa Magnus, agent d’une des moles du champignon de couche. Comptes rendus de l Académie des sciences (Ser D.) 274:1867–1870
    [Google Scholar]
  21. Lemke P. A. 1969; A century of compounds and their effect on fungi. Mycopathologia et mycologia applicata 38:49
    [Google Scholar]
  22. Lemke P. A., Ness T. M. 1970; Isolation and characterization of a double-stranded ribonucleic acid from Penicillium chrysogenum . Journal of Virology 6:813–819
    [Google Scholar]
  23. Lhoas P. 1971a; Infection of protoplast from Penicillium stoloniferum with double-stranded RNA viruses. Journal of General Virology 13:365–367
    [Google Scholar]
  24. Lhoas P. 1971b; Transmission of double-stranded RNA viruses to a strain of Penicillium stoloniferum through heterokaryosis. Nature, London 230:248–249
    [Google Scholar]
  25. Lhoas P. 1972; Mating pairs of Saccharomyces cerevisiae infected with double stranded RNA viruses from Aspergillus niger . Nature, London 236:86–87
    [Google Scholar]
  26. Moss M. O., Cole M. 1964; Reaction of 6-aminopenicillanic acid with carbohydrates and related substances. Biochemical Journal 92:643–648
    [Google Scholar]
  27. Nash C. H., Douthart R. J., Ellis L. F., Vanfrank R. M., Burnett J. P., Lemke P. A. 1973; On the mycophage of Penicillium chrysogenum . Canadian Journal of Microbiology 19:97–103
    [Google Scholar]
  28. Sermonti G. 1957; Analysis of vegetative segregation and recombination in Penicillium chrysogenum . Genetics 42:433–443
    [Google Scholar]
  29. Tuveson R. W., Peterson J. F. 1972; Virus-like particles in certain slow-growing strains of Neurospora crassa . Virology 47:527–531
    [Google Scholar]
  30. Vanderhaeghe H., Claesen M., Vlietinck A., Parmentier G. 1968; Specificity of penicillin acylase of Fusarium and of Penicillium chrysogenum . Applied Microbiology 16:1557–1563
    [Google Scholar]
  31. Wood H. A., Bozarth R. F. 1972; Properties of virus-like particles of Penicillium chrysogenum: One double-stranded RNA molecule per particle. Virology 47:604–609
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-76-2-265
Loading
/content/journal/micro/10.1099/00221287-76-2-265
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error