1887

Abstract

SUMMARY: A possible method using fluorescent brighteners for estimating the rate of wall accumulation by regenerating protoplasts is described. The relationship between the amount of wall and brightener adsorption was linear. Phases of slow and rapid brightener adsorption, thought to be correlated with chain and hypha formation respectively, were found during regeneration. The length of the slow phase varied according to the composition of the regeneration medium.

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/content/journal/micro/10.1099/00221287-74-2-281
1973-02-01
2022-01-26
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References

  1. Bachmann B. J., Bonner D. M. 1959; Protoplasts from Neurospora crassa. Journal of Bacteriology 78:550–556
    [Google Scholar]
  2. Bailey N. T. J. 1959 Statistical Methods in Biology London: English Universities Press;
    [Google Scholar]
  3. Bull A. T. 1970; Inhibition of polysaccharases by melanin. Enzyme inhibition in relation to mycolysis. Archives of Biochemistry and Biophysics 137:345–356
    [Google Scholar]
  4. Darken M. A. 1962; Absorption and transport of fluorescent brighteners by micro-organisms. Applied Microbiology 10:387–393
    [Google Scholar]
  5. Elorza M. V., Arst J. R. H. H., Cove D. J., Scazzocchio C. 1969; Permeability properties of Aspergillus nidulans protoplasts. Journal of Bacteriology 99:113–115
    [Google Scholar]
  6. Emerson S., Emerson M. R. 1958; Production and reversion of protoplast-like structures in the osmotic strain of Neurospora crassa. Proceedings of the National Academy of Sciences of the United States of America 44:668–671
    [Google Scholar]
  7. Garciá-Acha I., López-Belmonte F., Villanueva J. R. 1966; Regeneration of mycelial protoplasts of Fusarium culmorum. Journal of General Microbiology 45:515–523
    [Google Scholar]
  8. Gull K., Moore P. M., Trinci A. P. J. 1972; Formation of protoplasts from Geotrichum lactis and use of the fluorescence to detect cell walls. Transactions of the British Mycological Society 59:79–85
    [Google Scholar]
  9. Harrington B. J., Roper K. B. 1968; Use of a fluorescent brightener to demonstrate cellulose in the cellular slime molds. Applied Microbiology 16:106–113
    [Google Scholar]
  10. Kuster E., Williams S. T. 1964; Selection of media for the isolation of Streptomyces. Nature, London 202:928–929
    [Google Scholar]
  11. Maeda H., Ishida N. 1967; Specificity of binding of hexopyranosyl polysaccharides with fluorescent brightener. Journal of Biochemistry 62:276–278
    [Google Scholar]
  12. Nagata T., Takebe I. 1970; Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts. Planta 92:301–308
    [Google Scholar]
  13. Paton A. M., Jones S. M. 1971; Techniques involving optical brightening agents. In Methods in Microbiology vol 5A pp. 135–144 Edited by Norris J. R., Ribbons D. W. London and New York: Academic Press;
    [Google Scholar]
  14. Peberdy J. F. 1971; Protoplasts from Mortierella vinacea. Transactions of the British Mycological Society 56:67–72
    [Google Scholar]
  15. Peberdy J. F., Gibson R. K. 1971; Regeneration of Aspergillus nidulans protoplasts. Journal of General Microbiology 69:325–330
    [Google Scholar]
  16. Preece T. F. 1971; Fluorescent techniques in mycology. In Methods in Microbiology vol 4 pp. 509–516 Edited by Booth C. London and New York: Academic Press;
    [Google Scholar]
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