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Summary: We have discovered different host specificities in the z 20 and cf2004-6 strains of a particular Escherichia coli O4 serotype. The host specificities are controlled by a chromosomal locus linked to threonine. They are differentiated on the basis of results from plating efficiencies of phage ⊘,04, conjugation, and generalized transduction. The host specificities of E. coli strains k12 and cf2004-6 seem to be similar and distinct from that of z20.
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