1887

Microbiology Society logo

Volume 71, Issue 1

Sporulation in Bacillus subtilis. Characterization of Oligosporogenous Mutants and Comparison of Their Phenotypes with Those of Asporogenous Mutants Free

Abstract

SUMMARY: An investigation was undertaken to determine to what extent the properties of oligosporogenous (Osp) mutants allow them to be considered as a separate class of sporulation mutant, distinct from asporogenous (Sp) mutants. Of thirty Osp mutants examined, seventeen at least had a phenotype which had previously been identified with a Sp mutant. The majority of cells in an Osp culture either reached a particular stage in the sporulation process and then stopped, or in some cases went on to produce aberrant forms. Some of these aberrant forms have their counterparts in Sp mutants described by other authors, but some present new features. The morphological and biochemical sequences were linked so that if the majority of cells were blocked at a certain stage, then the biochemical sequence stopped accordingly. The general similarity in behaviour between the two types of mutant is consistent with the assumption that at least some of the Osp mutants have leaky mutations in genes where mutation can also give rise to Sp phenotypes. Evidence is presented to suggest that the ability of a cell of an Osp mutant to overcome its block, and so go on to form a spore, is a chance event when that stage in the process is reached. A mutant has been obtained in which the spores are octanol-resistant yet contain no measurable dipicolinate. In several other mutants the spores contained well-developed coat layers, but the cortex was poorly formed or completely missing.

  • Accepted:
  • Published Online:
© Society for General Microbiology, 1962
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-71-1-1
1972-06-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/71/1/mic-71-1-1.html?itemId=/content/journal/micro/10.1099/00221287-71-1-1&mimeType=html&fmt=ahah

References

  1. AUBERT J. P., MILLET J. 1961; Influence de l'alimentation carbonee sur le taux de sporulation de mutants de sporulation de Bacillus megaterium.. Compte rendu hebdomadaire des siances de VAcademie des sciences 253:1880–1882
     [Google Scholar]
  2. BALASSA G., YAMAMOTO T. 1970; Biochemical genetics of bacterial sporulation. III. Correlation between morphological and biochemical properties of sporulation mutants.. Molecular and General Genetics 108:1–22
     [Google Scholar]
  3. BOTT K. F., DAVIDOFF-ABELSON R. 1966; Altered sporulation and respiratory patterns in mutants of Bacillus subtilis induced by acridine orange.. Journal of Bacteriology 92:229–240
     [Google Scholar]
  4. BROWNLEE K. A., DELVES C. S., DORMAN M., GREEN C. A., GRENFELL E., JOHNSON J. D. A., SMITH N. 1948; The biological assay of streptomycin by a modified cylinder plate method.. Journal of General Microbiology 2:40–53
     [Google Scholar]
  5. COOTE J. G. 1972; Sporulation in Bacillus subtilis. Genetic analysis of oligosporogenous mutants.. Journal of General Microbiology 71:17–27
     [Google Scholar]
  6. DAWES I. W., KAY D., MANDELSTAM J. 1969; Sporulation in Bacillus subtilis. Establishment of a time scale for the morphological events.. Journal of General Microbiology 56:171–179
     [Google Scholar]
  7. DAWES I. W., KAY D., MANDELSTAM J. 1971; Determining effect of growth medium on the shape and position of daughter chromosomes and on sporulation in Bacillus subtilis.. Nature, London 230:567–569
     [Google Scholar]
  8. FORTNAGEL P., FREESE E. 1968; Analysis of sporulation mutants. II. Mutants blocked in the citric acid cycle.. Journal of Bacteriology 95:1431–1438
     [Google Scholar]
  9. Hanson R. S., Blicharska J., Szulmajster J. 1964; Relationship between the tricarboxylic acid cycle enzymes and sporulation in Bacillus subtilis.. Biochemical and Biophysical Research Communications 7:1–7
     [Google Scholar]
  10. HANSON R. S., MACKECHNIE I. 1969 Regulation of sporulation and the entry of carbon into the tricarboxylic acid cycle.. In Spores, vol IV pp. 196–211 Edited by Campbell L. L. Bethesda, Maryland:: American Society for Microbiology.;
     [Google Scholar]
  11. HANSON R. S., PETERSON J. A., YOUSTEN A. A. 1970; Unique biochemical events in bacterial sporulation.. Annual Review of Microbiology 24:53–90
     [Google Scholar]
  12. IICHINSKA E. 1960; Some physiological features of asporogenous mutants of bacilli. (English translation.). Microbiology (U.S.S.R.) 29:147–150
     [Google Scholar]
  13. KAY D., WARREN S. C. 1968; Sporulation in Bacillus subtilis. Morphological changes.. Biochemical Journal 109:819–824
     [Google Scholar]
  14. KORNBERG A., SPUDICH J. A., NELSON D. L., DEUTSCHER M. P. 1968; Origin of proteins in sporulation.. Annual Review of Biochemistry 37:51–78
     [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265–275
     [Google Scholar]
  16. MANDELSTAM J. 1969; Regulation of bacterial spore formation.. Symposium of the Society for General Microbiology 19:377–402
     [Google Scholar]
  17. MANDELSTAM J., WAITES W. M. 1968; Sporulation in Bacillus subtilis. The role of exo-protease.. Biochemical Journal 109:793–801
     [Google Scholar]
  18. MURRELL W. G. 1967; The biochemistry of the bacterial endospore.. Advances in Microbial Physiology 1:133–251
     [Google Scholar]
  19. RYTER A. 1965; Etude morphologique de la sporulation de Bacillus subtilis.. Annales de VInstitut Pasteur 108:40–60
     [Google Scholar]
  20. RYTER A., SCHAEFFER P., IONESCO H. 1966; Classification cytologique, par leur stade de blocage, des mutants de sporulation de Bacillus subtilis MARBURG.. Annales de VInstitut Pasteur 110:305–315
     [Google Scholar]
  21. SCHAEFFER P. 1969; Sporulation and the production of antibiotics, exoenzymes and exotoxins.. Bacteriological Reviews 33:48–71
     [Google Scholar]
  22. SCHAEFFER P., IONESCO H. 1960; Contribution a l'etude genetique de la sporogenese bacterienne.. Compte rendu hebdomadaire des seances de l'Academie des sciences 251:3125–3127
     [Google Scholar]
  23. SCHAEFFER P., IONESCO H., RYTER A., BALASSA G. 1965; La sporulation de Bacillus subtilis: etude genetique et physiol ogique.. Collogues internationaux du centre national de la recherche scientifique 124:553–563
     [Google Scholar]
  24. STERLINI J. M., MANDELSTAM J. 1969; Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance.. Biochemical Journal 113:29–37
     [Google Scholar]
  25. WAITES W. M., KAY D., DAWES I. W., WOOD D. A., WARREN S. C., MANDELSTAM J. 1970; Sporula- tion in Bacillus subtilis. Correlation of biochemical events with morphological changes in asporogenous mutants.. Biochemical Journal 118:667–676
     [Google Scholar]
  26. WARREN S. C. 1968; Sporulation in Bacillus subtilis. Biochemical changes.. Biochemical Journal 109:811–818.
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-71-1-1
Loading
Sporulation in Bacillus subtilis. Characterization of Oligosporogenous Mutants and Comparison of Their Phenotypes with Those of Asporogenous Mutants
Microbiology 71, 1 (1972); https://doi.org/10.1099/00221287-71-1-1
/content/journal/micro/10.1099/00221287-71-1-1
/content/journal/micro/10.1099/00221287-71-1-1
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error