1887

Abstract

SUMMARY

Pyronin Y (0·06 and 0·16 m), coumarin (2 and 4 m), 6,9-dimethyl 2-methyl-thiopurine (2 m) and caffeine (8 m), strongly inhibit Uvr repair (presumed excision repair) of u.v. lesions in . With 0·16 m-pyronin Y or 4 m-coumarin, one thymine dimer was a lethal event in the 12 strain 2926 and one to three dimers in the /r derivative -2 and the derivative -2. Neither these compounds nor 8 m-caffeine had a significant effect on the survival of irradiated bacteria of the corresponding strains -2 Hcr, -12, and -8. I suggest that 8 m-caffeine, 0·16 m-pyronin Y, 4 m-coumarin and 2 m-6,9-dimethyl 2-methylthiopurine selectively block excision repair without substantially affecting recombination repair.

Caffeine at 12 m only slightly depressed the viability of a u.v.-irradiated culture of -2 Hcr or -12, but at 16 m there was a much greater effect, particularly at low u.v. doses (< 40 ergs/mm). Since excision repair appeared to be effectively blocked by 8 m-caffeine, the depression of u.v. survival of the mutants by 16 m-caffeine was presumed to be due to interference with recombination repair (Witkin & Farquharson, 1969).

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/content/journal/micro/10.1099/00221287-70-2-221
1972-04-01
2022-01-28
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