1887

Abstract

SUMMARY: Electrophoretic patterns of esterases and other soluble proteins from two isolates of three of two of eight of C. three of , two of and four of were determined. Similar protein patterns were obtained for isolates representing a given bacterial species, with the exception of one isolate of which differed markedly from the other two isolates tested. All isolates possessed active esterases. None of the Corynebacterium isolates tested showed activity bands for dehydrogenases. Results demonstrated the applicability of the technique in differentiating between phytobacterial genera and between species of a given genus.

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1971-10-01
2024-04-26
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References

  1. Breed R. S., Murray E. G., Smith N. R. 1957 Bergey’s Manual of Determinative Bacteriology, 7th ed. Baltimore: Williams & Wilkins Co;
    [Google Scholar]
  2. Chang L. O., Srb A. M., Steward F. C. 1962; Electrophoretic separation of the soluble proteins of Neurospora. Nature; London: 193756–759
    [Google Scholar]
  3. Clare B. G. 1963; Starch-gel electrophoresis of proteins as an aid in identifying fungi. Nature; London: 200803–804
    [Google Scholar]
  4. Clare B. G., Flentje N. T., Atkinson M. R. 1968; Electrophoretic patterns of oxidoreductases and other proteins as criteria in fungal taxonomy. Australian Journal of Biological Science 21:275–295
    [Google Scholar]
  5. Clare B. G., Zentmyer G. A. 1966; Starch-gel electrophoresis of proteins from species of Phytophthora. Phytopathology 56:1334–1335
    [Google Scholar]
  6. Conn D. C., Willox M. E. 1965; Analysis of multimolecular enzymes as an aid to the identification of certain rapidly growing Mycobacteria, using starch-gel electrophoresis. Journal of Applied Bacteriology 28:165–173
    [Google Scholar]
  7. Davis B. J. 1964; Disc electrophoresis IE Method and application to human serum proteins. Annals, of New York Academy of Sciences 121:404–427
    [Google Scholar]
  8. Durbin R. D. 1966; Comparative gel-electrophoretic investigation of the protein patterns of Septoria species. Nature; London: 2101186–1187
    [Google Scholar]
  9. El-Sharkawy T. A., Huisingh D. 1968; Identification of Xanthomonas species by acrylamide-gel disc electrophoresis of soluble bacterial proteins. Phytopathology 58:1049 abstr
    [Google Scholar]
  10. Fowler R. C., Coble D. W., Crammer N. C., Brown T. McP. 1963; Starch-gel electrophoresis of a fraction of certain of the pleuropneumonia-like group of organisms. Journal of Bacteriology 86:1145–1151
    [Google Scholar]
  11. Gilbert L. I., Goldberg E. 1966; Metabolic relationships in the cockroach, Leucopheae moderae. II. Electrophoretic analysis of dehydrogenase activity in tissue extracts. Journal of Insect Physiology 12:53–63
    [Google Scholar]
  12. Gill H. S., Khare M. N. 1968; Disc electrophoresis of sonic extracts of five phytopathogenic bacteria. Phytopathology 58:1051–1052
    [Google Scholar]
  13. Gill H. S., Powell D. 1967; Differentiation of three species of Phytophthora by polyacrylamide-gel electrophoresis. Phytopathology 57:812
    [Google Scholar]
  14. Gottlieb D., Hepden Pamela M. 1966; The electrophoretic movement of proteins from various Streptomyces species as a taxonomic criterion. Journal of General Microbiology 44:95–104
    [Google Scholar]
  15. Hall R., Zentmyer G. A., Erwin D. C. 1969; Approach to taxonomy of Phytophthora through acrylamide-gel electrophoresis of proteins. Phytopathology 59:770–774
    [Google Scholar]
  16. Huisingh D., Durbin R. D. 1965; Acrylamide-gel electrophoresis of proteins from Agrobacterium species. Phytopathology 55:1062 abstr
    [Google Scholar]
  17. Huisingh D., Durbin R. D. 1967; Physical and physiological methods for differentiating among Agrobacterium rhizogenes, A. tumefaciens and A. radiobacter. Phytopathology 57:922–923
    [Google Scholar]
  18. Lovrekovich L., Kelment Z. 1960; Triphenyltetrazolium chloride tolerance of phytopathogenic bacteria. Phytopathologisches Zeitschrift 39:129–133
    [Google Scholar]
  19. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  20. Lund G. 1965; A comparison by the use of gel electrophoresis of soluble protein components and esterase enzymes of some group D streptococci. Journal of General Microbiology 40:413–419
    [Google Scholar]
  21. Morichi T., Sharpe M. E., Reiter B. 1968; Esterases and other soluble proteins of some lactic acid bacteria. Journal of General Microbiology 53:405–414
    [Google Scholar]
  22. Norris J. R. 1964; The classification of Bacillus thuringiensis. Journal of Applied Bacteriology 27:439–447
    [Google Scholar]
  23. Razin S. 1968; Mycoplasma taxonomy studied by electrophoresis of cell proteins. Journal of Bacteriology 96:687–694
    [Google Scholar]
  24. Razin S., Rottem S. 1967; Identification of Mycoplasma and other micro-organisms by polyacrylamide-gel electrophoresis of cell proteins. Journal of Bacteriology 94:1807–1810
    [Google Scholar]
  25. Robinson K. 1966; An examination of Corynebacterium spp. by gel electrophoresis. Journal Applied Bacteriology 29:179–184
    [Google Scholar]
  26. Rottem S., Razin S. 1967; Electrophoretic patterns of membrane proteins of Mycoplasma. Journal of Bacteriology 94:359–364
    [Google Scholar]
  27. Smith J. H., Powell D. 1968; A disc electrophoretic comparison of protein patterns of Erwinia amylovora with other bacteria, including associated yellow forms. Phytopathology 58:972–975
    [Google Scholar]
  28. Stipes R. J. 1967; Disc electrophoresis of mycelial proteins from Ceratocystis species. Phytopathology 57:833 abstr
    [Google Scholar]
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