SUMMARY: A strain of transformed interspecifically and intra-specifically for the gelatinase marker showed maximum competence in the early log phase of growth which was increased further by the addition of Casamino acids and Mg to the competence medium at pH 7.0. Ferric ions inhibited transformation and this effect was partially reversed by addition of EDTA. The DNA concentration for maximum frequency of transformation was about 20 μg./ml. The frequency of intraspecific transformation was ten times higher than that of interspecific transformation although the G + C mole per cent of the donor and recipient was very similar, 64.0 and 64.5 respectively. A surface-bound nuclease specific for the transforming DNA could have resulted in the high donor DNA requirement.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error