1887

Abstract

SUMMARY: Five streptomycins bleached the chloroplast system of ; the most effective were those which contained an aldehyde group in the streptose moiety: streptomycin and hydroxystreptomycin. Derivatives containing a secondary alcohol group in place of the aldehyde (dihydro-, dihydro-desoxy-, dihydrohydroxystreptomycin) require higher concentrations to elicit the same effect. Hydroxylamine reversed the bleaching effects of streptomycin and hydroxystreptomycin but not of dihydro-, dihydrodesoxy- or dihydrohydroxystreptomycin. These observations suggest that the aldehyde group of the streptomycins contributes to the action of these antibiotics on Euglena chloroplasts; the action of hydroxylamine would be to either remove the antibiotic from its site of action or to render it ineffective by reaction with the aldehyde group.

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/content/journal/micro/10.1099/00221287-59-2-203
1969-12-01
2024-04-26
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References

  1. Anderson L. A., Smillie R. M. 1966; Binding of chloramphenicol by ribosomes from chloroplasts.. Biochem. biophys. Res. Commun 23:535
    [Google Scholar]
  2. Arnon D. 1949; Copper enzymes.. Pl. Physiol 24:1
    [Google Scholar]
  3. Aronson J., Meyer W. L., Brock T. D. 1964; A molecular model for chemical and biological differences between streptomycin and dihydrostreptomycin.. Nature, Lond 202:555
    [Google Scholar]
  4. Davies J., Gilbert W., Gorini L. 1964; Streptomycin, suppression, and the code.. Proc. natn. Acad. Sci. U.S.A 51:883
    [Google Scholar]
  5. Ebringer L. 1966; Antibiotics and apochlorosis. I. Macrolide antibiotics—their common molecular structure responsible for bleaching of Euglena gracilis. . Folia microbiol., Praha 11:379
    [Google Scholar]
  6. Ebringer L., Mego J. L., Podová M. 1967; Reversal of streptomycin bleaching of Euglena gracilis by mutagenic concentrations of hydroxylamine.. Biochem. biophys. Res. Commun 29:571
    [Google Scholar]
  7. Gibor A., Granick S. 1962; Ultraviolet sensitive factors in the cytoplasm that affect the differentiation of Euglena gracilis. . J. Cell Biol 15:599
    [Google Scholar]
  8. Linnane A. W., Stewart P. R. 1967; The inhibition of chlorophyl formation in Euglena by antibiotics which inhibit bacterial and mitochondrial protein synthesis.. Biochem. biophys. Res. Commun 27:511
    [Google Scholar]
  9. Lyman H., Epstein H. T., Schiff J. A. 1961; Studies of chloroplast development in Euglena. I.Inactivation of green colony formation by u.v. light.. Biochim. biophys. Acta 50:301
    [Google Scholar]
  10. McCalla D. R. 1967; Isolation and properties of a Euglena mutant resistant to the killing and bleaching effects of N-methyl-N-nitro-N-nitrosoguanidine and some other agents.. Biochim. biophys. Acta 142:219
    [Google Scholar]
  11. Mego J. L. 1964; The effect of hadacidin on chloroplast development in non-dividing Euglena cells.. Biochim. biophys. Acta 79:221
    [Google Scholar]
  12. Mego J. L., Buetow D. E. 1967; Studies on chloroplast development in heat-bleached Euglena.. Le Chloroplaste, Croissance et Vieillissement Ed. by Sironval C. 274 Paris: Masson et Cie.;
    [Google Scholar]
  13. Pogo B. G. T., Pogo A. O. 1965; Inhibition by chloramphenicol of chlorophyll and protein synthesis and growth in Euglena gracilis. . J. Protozool 12:96
    [Google Scholar]
  14. Robbins W. J., Hervey A., Stebbins M. E. 1953; Euglena and vitamin B12.. Ann. N.Y. Acad. Sci 56:818
    [Google Scholar]
  15. Schiff J. A., Epstein H. T. 1965; The continuity of the chloroplast in Euglena.. Reproduction: Molecular, Subcellular, and Cellular Ed. by Locke M. 131 New York: Academic Press Inc.;
    [Google Scholar]
  16. Selsky M. I. 1967; Effects of puromycin aminonucleoside on growth and chloroplast development of Euglena gracilis. . Exp. Cell Res 47:237
    [Google Scholar]
  17. Stern J. L., Barner H. D., Cohen S. S. 1966; The lethality of streptomycin and the stimulation of RNA synthesis in the absence of protein synthesis.. J. molec. Biol 17:188
    [Google Scholar]
  18. Vazquez D., Monro R. E. 1967; Effects of some inhibitors of protein synthesis on the binding of aminoacyl t-RNA to ribosomal subunits.. Biochim. biophys. Acta 142:155
    [Google Scholar]
  19. Zahalsky A. C., Hutner S. H., Keane M., Burger R. M. 1962; Bleaching Euglena gracilis with the anti-histamines and streptomycin-type antibiotics.. Arch. Mikrobiol 42:46
    [Google Scholar]
  20. Zimmer C., Triebel H., Thrum H. 1967; Interaction of streptothricin and related antibiotics with nucleic acids.. Biochim. biophys. Acta 145:742
    [Google Scholar]
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