SUMMARY Optimal conditions, defined by the use of a known auxotrophic strain, are described for the mutagenesis of oidia by -methyl-- nitro--nitrosoguanidine (NTG), which is a very effective mutagen for bacteria and yeast. The oidia were best treated in phosphate buffer (pH 6·8) with a concentration of 15 g. NTG/ml. for about 70 min. The yield of auxo- trophs was considerably less than might be expected from a comparison of other systems in which NTG has been used as a mutagen.


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  1. Adelberg E. A., Mandel M., Chen G. C. C. 1965; Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli K12. Biochem. biophys. Res. Commun 18:788
    [Google Scholar]
  2. Megnet R. 1965; Screening of auxotrophic mutants of Schizosaccharomyces pombe with 2-deoxyglucose. Mutation Res 2:328
    [Google Scholar]
  3. Moore D. 1968; The effect of 2-deoxy-d-glucose on the growth and respiration of Coprinus lagopus . J. gen. Microbiol 52:433
    [Google Scholar]
  4. Morgan D. H. 1966; Suppression of ‘purple’ in Coprinus-an anomalous genetic situation. Genet. Res 7:195
    [Google Scholar]
  5. Nordström K. 1967; Induction of the petite mutation in Saccharomyces cerevisiae by N-methyl-N′-nitro-N-nitrosoguanidine. J. gen. Microbiol 48:277
    [Google Scholar]

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