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The Mutagenic Action of N-methyl-N′-nitro-N-nitrosoguanidine on Coprinus lagopus
Abstract
SUMMARY Optimal conditions, defined by the use of a known auxotrophic strain, are described for the mutagenesis of Coprinus lagopus oidia by N-methyl-N′- nitro-N-nitrosoguanidine (NTG), which is a very effective mutagen for bacteria and yeast. The oidia were best treated in phosphate buffer (pH 6·8) with a concentration of 15 μg. NTG/ml. for about 70 min. The yield of auxo- trophs was considerably less than might be expected from a comparison of other systems in which NTG has been used as a mutagen.
- Accepted:
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© Society for General Microbiology, 1969