1887

Abstract

SUMMARY: Optimal conditions, defined by the use of a known auxotrophic strain, are described for the mutagenesis of oidia by -methyl--nitro--nitrosoguanidine (NTG), which is a very effective mutagen for bacteria and yeast. The oidia were best treated in phosphate buffer (pH 6.8) with a concentration of 15 μg. NTG/ml. for about 70 min. The yield of auxotrophs was considerably less than might be expected from a comparison of other systems in which NTG has been used as a mutagen.

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/content/journal/micro/10.1099/00221287-55-1-121
1969-01-01
2019-10-22
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-55-1-121
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