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Volume 55, Issue 1

The Mutagenic Action of N-methyl-N′-nitro-N-nitrosoguanidine on Coprinus lagopus Free

Abstract

SUMMARY Optimal conditions, defined by the use of a known auxotrophic strain, are described for the mutagenesis of oidia by -methyl-- nitro--nitrosoguanidine (NTG), which is a very effective mutagen for bacteria and yeast. The oidia were best treated in phosphate buffer (pH 6·8) with a concentration of 15 g. NTG/ml. for about 70 min. The yield of auxo- trophs was considerably less than might be expected from a comparison of other systems in which NTG has been used as a mutagen.

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© Society for General Microbiology, 1969
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1969-01-01
2025-04-22
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References

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/content/journal/micro/10.1099/00221287-55-1-121
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The Mutagenic Action of N-methyl-N′-nitro-N-nitrosoguanidine on Coprinus lagopus
Microbiology 55, 121 (1969); https://doi.org/10.1099/00221287-55-1-121
/content/journal/micro/10.1099/00221287-55-1-121
/content/journal/micro/10.1099/00221287-55-1-121
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