1887

Abstract

SUMMARY: Morphological changes in harvested from 18 hr growth on nutrient agar surface and incubated in glucose saline at 37° have been studied by electron microscopy using metal shadowing, uranyl staining and thin sectioning techniques. Within the 6 hr incubation period, a vacuolar region has been found to separate the cell wall from the protoplasmic body presumably at one polar end. Subsequently such separation has been found all round the periphery of the protoplasmic body, which assumes a round form of average dimension 0.4 ± 0.05 μ. Within the 24 hr incubation period, majority of the cells (60 to 70 %) are rounded and of these a significant fraction (15 to 20 %) contained bodies limited by one single membrane. It is suggested that these bodies represent the protoplasts of

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-54-3-445
1968-12-01
2024-12-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/54/3/mic-54-3-445.html?itemId=/content/journal/micro/10.1099/00221287-54-3-445&mimeType=html&fmt=ahah

References

  1. BERNARD P.N., GALLUT J. 1943; Sur une mode de preparation de la toxine cholerique.. C. r. Séanc. Soc. Biol. 137:10
    [Google Scholar]
  2. BIRDSE D. C., COTA-ROBLES E. H. 1967; Production and ultrastruture of lysozyme and ethylenediaminetetraacetate-lysozymes pheroplasts of Escherichiu coli.. J. Buct. 93:427
    [Google Scholar]
  3. BORYSKO E, SAPRANAUSKAS P. 1954; A new technique for comparative phase contrast andelectron microscope studies of cells grown in tissue culture with an evaluation of the techniqueby means of time lapse cinemicrographs. Bull. Johns Hopkins Hosp. 95:68
    [Google Scholar]
  4. BRENNER S., DARK F.A, GERHARDT P., JEYENS M.H., KANDLER O., KELLENBER E., KELLENBER-NOBEL E., MCQUILLE K., RUBIO-HUERT M., SALTON M. R. J., STRANGE R.E., TOMCSI J., WEIBULL C. 1958; Bacterial protoplasts.. Nature, Lond. 181:1713
    [Google Scholar]
  5. CHATTERJEE S.N., DAS J. 1966; Secretory activity of Vibrio cholerae as evidenced by electron microscopy.. In Electron Microscopy. Ed. by Uyeda R. 2: p. 259 Tokyo:: Maruzen Co. Ltd;
    [Google Scholar]
  6. CHATTERJEE S.N., DAS J. 1967; Electron microscoPlc observations on the excretion of cellwall material by Vibrio cholerue. 49:1
    [Google Scholar]
  7. COSTERTON J.W., FORSBERG C., MATULA T.I., BUCKMIRE F.L.A., MACLEOD R.A. 1967; Nutrition and metabolism of marine bacteria.. J. Buct. 94:1764
    [Google Scholar]
  8. GALLUT J. 1954; Contribution á l’étude de la toxine cholerique: variation du pouvoir toxique de Vibrio cholerae Ogawa en cours de la maladie.. Annls Znst. Pusteur, Paris: 86:561
    [Google Scholar]
  9. GALLUT J. 1965; Antigenic structure of vibrios. Proc. Cholera Res.. Symp. Honolulu, p. 235
    [Google Scholar]
  10. GALLUT J., GIUNTINE J. 1963; Étude de Vibrio cholerue au microscope klectronique et relation entre l’aspect morphologique et l‘agglutination ‘O’.. Bull. Wld Hlth Org 29:767
    [Google Scholar]
  11. HINES W.D., FREEMABN B.A., PEARSON G.R. 1964; Production and characterizationo f Brucell spheroplasts.. J. Buct. 87:438
    [Google Scholar]
  12. HOFSCHNEIDER P. H. 1960; Zur Wandstruktur von Escherichiu coli B Sphaeroplasten. Proc. European Regional Conf. Electron Microscopy, Delft 2: p. 1028
    [Google Scholar]
  13. JEYNES M.H. 1961; The growth and division of bacterial protoplasts.. Exp. Cell Res 24:255
    [Google Scholar]
  14. KELLENBERG E., RYTER A. 1964; In Modern Developments in Electron Microscopy.. Ed. by Siegel B. M. p. 355 New York and London:: Academic Press.;
    [Google Scholar]
  15. KELLENBERGER E., RYTER A., SECHAND J. 1958; ElectronmicroscoPlc study of DNA plasma.. J. biophys. biochem. Cytol. 4:671
    [Google Scholar]
  16. Lawn A.M. 1960; The use of potassium permanganate as an electron dense stain for sections of tissue embedded in epoxy resin.. J. biophys. biochem. Cytol. 7:197
    [Google Scholar]
  17. LEDERBERG J. 1956; Bacterial protoplasts. Proc. nutn. Acad.. Sci. U.S.A 42:576
    [Google Scholar]
  18. LUFT J. H. 1961; Improvements in epoxy resin embedding methods.. J. biophys. biochem. Cytol. 9:409
    [Google Scholar]
  19. MCQUILLEN K. 1958; Lysis resulting from metabolic disturbance. J. gen. Microbiol I8:498
    [Google Scholar]
  20. MCQUILLEN K. 1960; Bacterial protoplasts. In The Bacteria.. Ed. by Gunsalus I. C., Stanier R. Y. p. 249 New York:: Academic Press Inc.;
    [Google Scholar]
  21. MILLONING G. 1961; A modified procedure for lead staining of thin sections. J. biophys. biochem.Cytol 111:736
    [Google Scholar]
  22. MITCHELL P., MOYLE J. 1956; ‘protoplast’ release in Bacterium coli. Nature, Lond. 178:993
    [Google Scholar]
  23. SALTON M. R. J. 1961; The anatomy of the bacterial surface. Bact. Rev 125:77
    [Google Scholar]
  24. STOLP H., STARR M.P. 1965; Bacteriolysis. A.. Rev. Microbiol. 19:79
    [Google Scholar]
  25. THORSSON K.C., WEIBULL C. 1958; S tudies on the structure of bacterial L forms, protoplasts and protoplast like bodies.. J.Ultrastruct. Res. 1:412
    [Google Scholar]
  26. WATSON M. L. 1957 1957; R eduction of heating artifacts in thin sections examined in electron microscope.. J. biophys. biochem. Cytol. 3:1017
    [Google Scholar]
  27. WEIBULL C. 1953; The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme.. J. Bact. 66:688
    [Google Scholar]
/content/journal/micro/10.1099/00221287-54-3-445
Loading
/content/journal/micro/10.1099/00221287-54-3-445
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error