1887

Abstract

SUMMARY

We have studied the effect of distamycin A on the synthesis of inducible enzymes in strain K12. Distamycin A did not inhibit the growth of K12 when the micro-organism already contained constitutive or inducible enzymes for the metabolism of sugars. The growth of was prevented when distamycin A was present during the enzymic induction. Distamycin A inhibited the formation of inducible β-galactosidase; the activity of the antibiotic was directly related to its concentration, but independent of the concentration of the inducer. Thus it does not seem that distamycin A causes a decrease of the concentration of inducer. The enzyme synthesized in the presence of subinhibitory concentrations of distamycin A showed the same kinetic behaviour as β-galacto-sidase induced under normal conditions.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-43-3-369
1966-06-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/43/3/mic-43-3-369.html?itemId=/content/journal/micro/10.1099/00221287-43-3-369&mimeType=html&fmt=ahah

References

  1. Arcamone F., Penco S., Nicolella V., Orezzi P., Pirelli M. A. 1964; The structure and synthesis of distamycin A. Nature, Lond 203:1064
    [Google Scholar]
  2. Barr H. J. 1963; A new genetical particle: the hypotheson. J. theoret. Biol 3:514
    [Google Scholar]
  3. Casazza A. M., Ghione M. 1964; Therapeutic action of distamycin A on vaccinia virus infections in vivo. Chemotherapia 9:80
    [Google Scholar]
  4. Davis B. D., Mingioli E. S. 1950; Mutants of E. coli requiring methionine or vitamin B12. J. Bact 60:17
    [Google Scholar]
  5. Di Marco A., Gaetani M., Orezzi P., Scotti T., Arcamone F. 1962; Experimental studies on distamycin A. A new antibiotic with cytotoxic activity. Cancer Chemoth. Rep 18:16
    [Google Scholar]
  6. Di Marco A., Ghione M., Sanfilippo A., Morvillo E. 1963a; Selective inhibition of the multiplication of phage Tl in E. coli k 12. Experientia 19:134
    [Google Scholar]
  7. Di Marco A., Ghione M., Migliacci A., Morvillo E., Sanfilippo A. 1963; Studi sul meccanismo dell'azione antifagica dell'antibiotico distamicina A. G. Microbiol. 11:87
    [Google Scholar]
  8. Jacob F., Monod J. 1961; Genetic regulatory mechanisms in the synthesis of protein. J. mol. Biol 3:318
    [Google Scholar]
  9. Jacob F., Perrin D., Sanches C., Monod J. 1960; L’opèron: groupe de gènes à expression coordonnèe par un opérateur. C. r. hebd. Séanc. Acad. Sci., Paris 250:1227
    [Google Scholar]
  10. Lederberg J. 1950; The β-d-galactosidase of E. coli strain k 12. J. Bact 60:381
    [Google Scholar]
  11. Lineweaver H., Burk D. 1934; The determination of enzymes dissociation constants. J. Am. chem. Soc 56:658
    [Google Scholar]
  12. Monod J., Cohn M. 1952; La biosynthèse induite des enzymes. Advanc. Enzym 13:62
    [Google Scholar]
  13. Verini M. A., Ghione M. 1964; Activity of distamycin A on cell cultures infected with vaccinia virus. Chemotherapia 9:144
    [Google Scholar]
  14. Werner G. H., Ganter P., de Ratuld Y. 1964; Studies on the antiviral activity of distamycin A. Chemotherapia 9:65
    [Google Scholar]
  15. Woolley D. W. 1952 A Study on Metabolites. New York: J. Wiley and Sons;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-43-3-369
Loading
/content/journal/micro/10.1099/00221287-43-3-369
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error