1887

Abstract

SUMMARY

Resistant strains of were obtained by one-step selection on high concentrations of various inhibitors; each strain was mutant in a different gene conferring resistance to actidione (), -fluoro-phenylalanine (), teoquil (), iodoacetate () or malachite green (). Some mutant alleles have been firmly, others tentatively, located. For comparative purposes attempts were made to find instances of multi-step or non-genic increases in resistance to malachite green and to teoquil, by prolonged exposure to low concentrations of inhibitor. No such increases were found.

is fully dominant, is semidominant, , tel and are recessive. confers resistance to acriflavine to about the same degree as the non-allelic but these two mutant alleles do not show additivity. confers resistance to iodoacetate and also suppresses requirement for nicotinic acid (). strains, which are not resistant to fluoroacetate, are able to use acetate as sole carbon source.

Nutritionally balanced heterokaryons, between and sensitive strains, show a gradual (and reversible) increase of the component on increasing actidione concentrations. Ultimately a plateau is reached; this presumably represents the nutritional limits of each particular combination of nutritional markers.

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1965-08-01
2021-07-29
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References

  1. Apirion D. 1962; A general system for the automatic selection of auxotrophs from prototrophs and vice versa in micro-organisms. Nature, Lond 195:959
    [Google Scholar]
  2. Bartlett G. W. 1959; Gain and loss of resistance in the fungus Penicillium roqueforti. Proc. roy. Soc. B 195120
    [Google Scholar]
  3. Cavalli L. L., Maccacaro G. A. 1952; Polygenic inheritance of drug resistance in the bacterium Escherichia coli. Heredity 6:311
    [Google Scholar]
  4. Clutterbuck A. J. 1964; Heterokaryosis in Aspergillus nidulans. Ph.D. Thesis University of Sheffield;
    [Google Scholar]
  5. Collier H. O. J., Potter M. D., Taylor B. P. 1955; Antifungal activities of bisi-soquinolinium and bisquinolinium salts. Br. J. Pharmacol 195:343
    [Google Scholar]
  6. Davis B. D. 1955; Intermediates in amino acid biosynthesis. Advanc. Enzymol 16:247
    [Google Scholar]
  7. Davies J., Gilbert W., Gorini L. 1964; Streptomycin, suppression and the code. Proc. nat. Acad. Sci., Wash 51883
    [Google Scholar]
  8. Dean A. C. R., Hinshelwood C. 1952; The resistance of Bacterium lactis aerogenes to proflavine (2 : 8 diaminoacridine). 1. The applicability of the statistical fluctuation test. Proc. roy. Soc. B 195236
    [Google Scholar]
  9. Dean A. C. R., Hinshelwood C. 1954; The stability of various adaptations of Bacterium lactis aerogenes (Aerobacter aerogenes). Proc. roy. Soc. B 19545
    [Google Scholar]
  10. de Palma C., Morpurgo G. 1963; Location of pfp-1. Aspergillus News Letter 4:11
    [Google Scholar]
  11. Forbes E. 1959; Use of mitotic segregation for assigning genes to linkage groups in Aspergillus nidulans. Heredity 13:67
    [Google Scholar]
  12. Gots J. S., Sevag M. G. 1948; Enzymatic studies on the mechanism of the resistance of pneumococcus to drugs. I. Studies of the dehydrogenase activities and inter-relationships of pneumococci susceptible and resistant to acriflavine, optochin, propamidine and sulphonamides. J. Bact 56:709
    [Google Scholar]
  13. Haas E. 1944; The effect of atabrine and quinine on isolated respiratory enzymes. J. biol. Chem 195:321
    [Google Scholar]
  14. Hellerman L., Lindsay A., Bovarnick M. R. 1946; Inhibition of d-amino acid oxidase by competition with F.A.D. by atabrine, quinine and certain other drugs. J. biol. Chem 195:553
    [Google Scholar]
  15. Hessler A. Y. 1963; Acridine resistant mutants of T2h bacteriophage. Genetics 48:1107
    [Google Scholar]
  16. Hotchkiss R. D., Evans A. H. 1958; Analysis of the complex sulfonamide resistance locus of Pneumococcus. Cold Spr. Harb. Symp. quant. Biol 195:85
    [Google Scholar]
  17. Jacob F., Monod J. 1961; Genetic regulatory mechanisms and the synthesis of proteins. J. mol. Biol 3:318
    [Google Scholar]
  18. Jinks J. L. 1952; Heterokaryosis: a system of adaptation in wild fungi. Proc. roy. Soc. B 19583
    [Google Scholar]
  19. Kacser H. 1963; The kinetic structure of organisms. In Biological Organization Harris R. J. C. 25 New York: Academic Press;
    [Google Scholar]
  20. Käfer E. 1958; An 8-chromosome map of Aspergillus nidulans. Advanc. Genet 9:105
    [Google Scholar]
  21. Lewis D. 1963; A structural gene for the raethionine-activating enzyme and its mutation as a cause of resistance to ethionine. Nature, Lond 195:151
    [Google Scholar]
  22. Lhoas P. 1961; Mitotic haploidisation by treatment of Aspergillus niger diploids with p-fluorophenylalanine. Nature, Lond 195:734
    [Google Scholar]
  23. Mcilwain H. 1941; A nutritional investigation of the antibacterial action of acriflavine. Biochem. J 35:1311
    [Google Scholar]
  24. Morpurgo G. 1961a; Somatic segregation induced by p-fluorophenylalanine. Aspergillus News Letter 2:4
    [Google Scholar]
  25. Morpurgo G. 1961b; Resistance to antimetabolites in Aspergillus nidulans. Aspergillus News Letter 2:9
    [Google Scholar]
  26. Pollock M. R. 1953; Stages in enzyme adaptation. In Adaptation in micro-organismsSymp. Soc. gen. Microbiol 3150
    [Google Scholar]
  27. Pontecorvo G. 1963a; Glasgow list of located or partially located mutants of Aspergillus nidulans. Aspergillus News Letter 4:12
    [Google Scholar]
  28. Pontecorvo G. 1963b; Microbial genetics: retrospect and prospect. Proc. roy. Soc. B 1951
    [Google Scholar]
  29. Pontecorvo G., Roper J. A., Hemmons L. M., MacDonald K. D., Bufton A. W. J. 1953; The genetics of Aspergillus nidulans. Advanc. Genet 5:141
    [Google Scholar]
  30. Roper J. A., Käfer E. 1957; Acriflavine resistant mutants of Aspergillus nidulans. J. gen. Microbiol 16:660
    [Google Scholar]
  31. Sevag M. G., Gots J. S. 1948a; Enzymatic studies on the mechanism of the resistance of pneumococcus to drugs. II. The inhibition of dehydrogenase activities by drugs; antagonistic effects of riboflavin to inhibitions. J. Bact 56:723
    [Google Scholar]
  32. Sevag M. G., Gots J. S. 1948b; Enzymatic studies on the mechanism of the resistance of pneumococcus to drugs. III. Experimental results indicating alteration in enzyme proteins associated with the development of resistance to drugs. J. Bact 56:737
    [Google Scholar]
  33. Siegel M. R., Sisler H. D. 1963; Inhibition of protein synthesis in vitro by cyclo-heximide. Nature, Lond 200:675
    [Google Scholar]
  34. Wild D. G., Hinshelwood C. 1955; The development of drug resistance in strains of Sacckaromyces cerevisiae: resistance to 2:4-dinitrophenol and brilliant green. Proc. roy. Soc. B 144287
    [Google Scholar]
  35. Yanofsky C., Helsinski D. R., Maling B. D. 1961; The effects of mutation on the composition and properties of the A protein of Escherichia coli tryptophan synthetase. Cold Spr. Harb. Symp. quant. Biol 26:11
    [Google Scholar]
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