1887

Abstract

SUMMARY: The rates of formation of transformed cells in mixtures of transforming DNA and transformable bacteria have been measured. The data were interpreted as follows. About 4–5 sec. are required for the penetration of a DNA particle of 8–9 × 10 mean molecular weight. The average number of penetration sites per bacterium is probably two. There is no reversible attachment of DNA to these sites. Crude calculations show that about one out of every two encounters between a bacterium and a DNA molecule results in the penetration of the latter into the cell.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-35-3-391
1964-06-01
2022-05-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/35/3/mic-35-3-391.html?itemId=/content/journal/micro/10.1099/00221287-35-3-391&mimeType=html&fmt=ahah

References

  1. Alexander H. E., Leidy G. 1951; Determination of inherited traits of H. influenzae by desoxyribonucleic acid fractions from type-specific cells.. J. exp. Med.93–345
    [Google Scholar]
  2. Doty P., McGill B. B., Rice S. A. 1958; The properties of sonic fragments of deoxyribose nucleic acid.. Proc. nat. Acad. Sci., Wash.44–432
    [Google Scholar]
  3. Elias H. G. 1962; Munchen: Beckman Instruments GmbH.. In Ultrazentrifugen-Methoden, p. 116
    [Google Scholar]
  4. Fox M. S., Hotchkiss R. D. 1957; Initiation of bacterial transformation.. Nature, Lond.179–1322
    [Google Scholar]
  5. Goodgal S. H., Herriott R. M. 1961a; Studies on transformation of Haemophilus influenzae I. Competence.. J. gen. Physiol.44–1201
    [Google Scholar]
  6. Goodgal S. H., Herriott R. M. 1961b; Studies on transformation of Haemophilus influenzae II. The molecular weight of transforming DNA by sedimentation and diffusion measurements.. J. gen. Physiol.44–1299
    [Google Scholar]
  7. Leidy G., Jaffee I., Alexander H. E. 1962; Emergence of competence (for transformation) of three Haemophilus species in a chemically defined environment.. Proc. Soc. exp. Biol., N.Y.III–725
    [Google Scholar]
  8. Lerman L. S., Tolmach L. J. 1957; Genetic transformation I. Cellular incorporation of DNA accompanying transformation in Pneumococcus.. Biochim. hiophys. Acta,26–68
    [Google Scholar]
  9. Smoluchowski M. v. 1917; Versuch einer mathematischen Theorie der Koagulations-kinetik kolloider Losungen.. Z. phys. Chem.92–129
    [Google Scholar]
  10. Stuy J. H. 1959; Studies on the mechanism of radiation inactivation of microorganisms V. Deoxyribonucleic acid metabolism in ultra-violet irradiated Haemophilus influenzae.. J. Bad.78–49
    [Google Scholar]
  11. Stuy J. H. 1960; Studies on the mechanism of radiation inactivation of microorganisms VI. X-ray-induced breakdown of deoxyribonucleic acid in Haemophilus influenzae and in other bacteria.. J. Bad.79–707
    [Google Scholar]
  12. Stuy J. H. 1962; Transformability of Haemophilus influenzae.. J. gen. Microbiol.29–537
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-35-3-391
Loading
/content/journal/micro/10.1099/00221287-35-3-391
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error