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Abstract
SUMMARY: The rumen oligotrich protozoa, Eremoplastron bovis (Dogiel, 1927), E. brevispinum (Kofoid & MacLennan, 1932), Entodinium longinucleatum (Dogiel, 1925), E. ovinum (Dogiel, 1927), Eudiplodinium maggii (Fiorentini, 1889) and various Epidinium species have been cultured in mixed culture for over 7 months. The medium consisted of rice starch, dried grass and casein in a mineral salt solution + rumen liquor. No antibiotics were used. Preliminary studies showed that sodium sulphide causes inhibition of protozoal metabolism in concentrations greater than 0·01 %. Pure cultures of Epidinium ecaudatum (Fiorentini, 1889) and Eremoplastron bovis have been established. In addition, a clone of E. bovis was maintained for 5 months. A method for growing quantities of protozoa for biochemical studies is described.
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