SUMMARY: The rumen oligotrich protozoa, (Dogiel, 1927), (Kofoid & MacLennan, 1932), (Dogiel, 1925), (Dogiel, 1927), (Fiorentini, 1889) and various Epidinium species have been cultured in mixed culture for over 7 months. The medium consisted of rice starch, dried grass and casein in a mineral salt solution + rumen liquor. No antibiotics were used. Preliminary studies showed that sodium sulphide causes inhibition of protozoal metabolism in concentrations greater than 0·01 %. Pure cultures of (Fiorentini, 1889) and have been established. In addition, a clone of was maintained for 5 months. A method for growing quantities of protozoa for biochemical studies is described.


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