SUMMARY: Myxamoebae of were grown in a peptone + yeast extract + glucose broth to which formalin-killed or had been added. Either the formalin-killed bacteria or a fraction from broken bacteria was indispensable for growth. The activity of the bacteria was destroyed by heat. The supernatant fluid of sonically disrupted bacteria supported growth; the active fraction of this was non-dialysable and was precipitated by ammonium sulphate. Lysed bacterial protoplasts did not support growth. Numerous compounds were tested unsuccessfully in attempting to eliminate the bacterial factor. When glucose was omitted from the medium the initialgrowth rate did not change but the population began to decrease rapidly after the fifth day.


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