1887

Abstract

SUMMARY: Myxamoebae of were grown in a peptone + yeast extract + glucose broth to which formalin-killed or had been added. Either the formalin-killed bacteria or a fraction from broken bacteria was indispensable for growth. The activity of the bacteria was destroyed by heat. The supernatant fluid of sonically disrupted bacteria supported growth; the active fraction of this was non-dialysable and was precipitated by ammonium sulphate. Lysed bacterial protoplasts did not support growth. Numerous compounds were tested unsuccessfully in attempting to eliminate the bacterial factor. When glucose was omitted from the medium the initialgrowth rate did not change but the population began to decrease rapidly after the fifth day.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-32-3-409
1963-09-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/32/3/mic-32-3-409.html?itemId=/content/journal/micro/10.1099/00221287-32-3-409&mimeType=html&fmt=ahah

References

  1. Adam K. M. G. 1959; The growth of Acanthamoeba sp. in a chemically defined medium. J. gen. Microbiol 21:519
    [Google Scholar]
  2. Band R. N. 1959; Nutritional and related biological studies on the free-living soil amoeba, Hartmanella rhysodes.. J. gen. Microbiol 21:80
    [Google Scholar]
  3. Brent M. M. 1954; Nutritional studies on the amoebo-flagellate, Tetramitus rostratus.. Biol. Bull., Woods Hole 106:269
    [Google Scholar]
  4. Cailleau R. 1933; Culture d'Acanthamoeba castellanii en milieu liquide. C.R. Soc. Biol., Paris 113:990
    [Google Scholar]
  5. Cohen A. L. 1939; Nutrition of the myxomycetes. I. Pure culture and two-membered culture of myxomycete plasmodia. Bot. Gaz 101:243
    [Google Scholar]
  6. Daniel J. W., Rusch H. P. 1961; The pure culture of Physarum polycephalum on a partially defined soluble medium. J. gen. Microbiol 25:47
    [Google Scholar]
  7. Gebicki J. M., James A. M. 1960; The preparation and properties of spheroplasts of Aerobacter aerogenes.. J. gen. Microbiol 23:9
    [Google Scholar]
  8. Hok K. A. 1954; Studies of the nutrition of myxomycete plasmodia. Amer. J. Bot 41:792
    [Google Scholar]
  9. Kerr N. S., Sussman M. 1958; Clonal development of the true slime mould, Didymium nigripes.. J. gen. Microbiol 19:173
    [Google Scholar]
  10. Kerr N. S. 1959; Actidione-resistant mutants of Didymium nigripes.. J. Protozool 6: suppl 16
    [Google Scholar]
  11. Kerr N. S. 1961; A study of plasmodium formation by the true slime mold, Didymium nigripes.. Exp. Cell Res 23:603
    [Google Scholar]
  12. Neff R. J. 1957; Purification, axenic cultivation, and description of a soil amoeba, Acanthamoeba sp. J. Protozool 4:176
    [Google Scholar]
  13. Neff R. J., Neff R. H., Taylor R. E. 1958; The nutrition and metabolism of a soil amoeba, Acanthamoeba sp. Physiol. Zool 31:73
    [Google Scholar]
  14. Reich K. 1935; The cultivation of a sterile amoeba on media without solid food. J. exp. Zool 69:497
    [Google Scholar]
  15. Sobels J. C. 1950; Nutrition de quelques myxomycètes en cultures pures et associées et leurs propriétés antibiotiques. Antonie van Leeuwenhoek 16:123
    [Google Scholar]
  16. Spizizen J. 1962; Preparation and use of protoplasts. In Methods in Enzymology Ed. by Colowick S. P., Kaplan N. O. 5122 New York: Academic Press, Inc;
    [Google Scholar]
  17. Sussman M., Bradley S. G. 1954; A protein growth factor of bacterial origin required by the cellular slime molds. Arch. Biochem. Biophys 51:428
    [Google Scholar]
  18. Sussman M. 1963; Growth of the cellular slime mold, Polysphondylium pallidum in a simple nutrient medium. Science 139:338
    [Google Scholar]
  19. Weinstein P. P., Jones M. F. 1956; The in vitro cultivation of Nippostrongylus muris to the adult stage. J. Parasitol 42:215
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-32-3-409
Loading
/content/journal/micro/10.1099/00221287-32-3-409
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error