SUMMARY: The state of colicinogenic factors E, I and V () In Escherichia coli K 12 studied. The analysis of the results of conjugation experiments involving different Hfr or F and F strains shows that: (1) The frequency transfer of these colicinogenic factors differs markedly: is tr with maximum efficiency, and , on the contrarv, only at low frequency. (2) Each colicinogenic factor is transferred from different type of Hfr or F strains with similar frequencies. (3) They linked with chromosomal markers. (4) The non-colicinogenic character of the donor parent is never transmitted to recombinants. (5) Zygotes that have received , transmit it to all daughter cells. All these ults lead to the conclusion that colicinogenic factors E I and V are in an extrachromosomal state in F and Hfr bacteria. The same; would apply to their state in F bacteria, since the results with inogenic factor V seem to indicate that this factor replicates in of bacteria autonomously and at a faster rate than the chromosome.


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