1887

Abstract

SUMMARY: Genetic transformation was investigated among spp. whose normal habitat is the nasopharynx of humans. Seven species, as characterized in (1957), were represented. Deoxyribonucleate (DNA) preparations from streptomycin-resistant mutants of , and conferred resistance upon streptomycin-susceptible parent strains of the corresponding species (intraspecific transformation) and of each other species (interspecific transformation). Ratios of interspecific to intraspecific transformation were 0.01 or higher for all possible combinations of DNA and recipient cells of the six species. On the other hand, cells, which exhibited high frequencies of intraspecific transformation, were not transformed at detectable frequencies by DNA from any of the six species listed above. In turn, DNA from had little or no transforming activity for these other neisseriae.

Possible evidence of structural differences between these DNA's was sought by analysing the base contents of transforming preparations. The bases adenine, thymine, guanine and cytosine were present in about equal proportions in the DNA's of the six and . In DNA preparations from two strains of , however, adenine and thymine predominated. The ratio (adenine + thymine/guanine + cytosine) was higher than 1.4 compared to 1.0 for the others.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-26-2-303
1961-10-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/26/2/mic-26-2-303.html?itemId=/content/journal/micro/10.1099/00221287-26-2-303&mimeType=html&fmt=ahah

References

  1. Alexander H. E., Redman W. 1953; Transformation of type specificity of meningococci.. J. exp. Med. 97:797
    [Google Scholar]
  2. Austrian R. 1952; Observations on the transformation of pneumococcus in vivo. Johns Hopk. Hosp. Bull. 91:189
    [Google Scholar]
  3. Bendich A. 1957; Methods for characterization of nucleic acids by base composition.. In Methods in Enzymology Ed. by Colowick S. P., Kaplan N. C. 3 p 715 New York: Academic Press Inc;
    [Google Scholar]
  4. Bergey's Manual of Determinative Bacteriology (1957), 7th ed. Ed. by Breed E. G. D., Murray N. R., Smith. Baltimore: The Williams and Wilkins Co;
    [Google Scholar]
  5. Bracco R. M., Krauss M. R., Roe A. S., MacLeod C. M. 1957; Transformation reactions between pneumococcus and three strains of streptococci.. J. exp. Med. 106:247
    [Google Scholar]
  6. Branham S. E. 1930; A new meningococcus-like organism (Neisseria flavescens n.s. p.) from epidemic meningitis. Publ. Hlth Rep., Wash. 45:845
    [Google Scholar]
  7. Catlin B. W. 1960a; Transformation of Neisseria meningitidis by deoxyribonucleates from cells and from culture slime. J. Bact. 79:579
    [Google Scholar]
  8. Catlin B. W. 1960b; Interspecific transformation of Neisseria by culture slime containing deoxyribonucleate. Science, 131:608
    [Google Scholar]
  9. Catlin B. W. 1960c; Transformation reactions within and between species of Neisseria. Bact. Proc p 74
    [Google Scholar]
  10. Catlin B. W. 1961; Affinities among Neisseria as revealed by studies of DNAs and DNases.. Bact. Proc p 90
    [Google Scholar]
  11. Catlin B. W., Cunningham L. S. 1958; Studies of extracellular and intracellular bacterial deoxyribonucleic acids.. J. gen. Microbiol. 19:522
    [Google Scholar]
  12. Dische Z. 1955; Color reactions of nucleic acid components.. In The Nucleic Adds Ed. by Chargaff E., Davidson J. N. 1 p 285 New York: Academic Press Inc;
    [Google Scholar]
  13. Green D. M. 1959; A host-specific variation affecting relative frequency of transformation of two markers in pneumococcus.. Exp. Cell Res. 18:466
    [Google Scholar]
  14. Hajek J. P., Pelczar M. J., Faber J. E. 1950; Variations in the fermentative capacity of neisseriae.. Amer. J. din. Path. 20:630
    [Google Scholar]
  15. Jessen J. 1934; Studien über gramnegative Kokken.. Zbl. Bakt Abt. 1 133:75
    [Google Scholar]
  16. Lanni F. 1960; Genetic significance of microbial DNA composition.. Perspectives in Biol. Med. 3:418
    [Google Scholar]
  17. Lee K. Y., Wahl R., Barbu E. 1956; Contenu en bases puriques et pyrimidiques des acides désoxyribonucléiques des bactéries.. Ann. Inst. Pasteur, 91:212
    [Google Scholar]
  18. Leidy G., Hahn E., Alexander H. E. 1956; On the specificity of the desoxyribonucleic acid which induces streptomycin resistance in Hemophilus. J. exp. Med. 104:305
    [Google Scholar]
  19. Leidy G., Hahn E., Alexander H. E. 1959; Interspecific transformation in Hemophilus; a possible index of relationship between H. influenzae and H. aegyptius. Proc. Soc. exp. Biol., N.Y. 102:86
    [Google Scholar]
  20. Ottolenghi E., Hotchkiss R. D. 1960; Appearance of genetic transforming activity in pneumococcal cultures.. Science, 132:1257
    [Google Scholar]
  21. Pelczar M. J., Doetsch R. N. 1949; On the direct fermentation of maltose.. Science 110:256
    [Google Scholar]
  22. Ravin A. W. 1960; The origin of bacterial species.. Genetic recombination and factors limiting it between bacterial populations Bad. Rev. 24:201
    [Google Scholar]
  23. Schaeffer P. 1958; Interspecific reactions in bacterial transformation.. Symp. Soc. exp. Biol. 12:60
    [Google Scholar]
  24. Smith J. D., Wyatt G. R. 1951; The composition of some microbial deoxypentose nucleic acids.. Biochem. J. 49:144
    [Google Scholar]
  25. Subcommittee on the family Neisseriaceae (1954). Preliminary report Int. Bull. bact. Nomen. Taxon. 4:95
    [Google Scholar]
  26. Topley and Wilson's Principles of Bacteriology and Immunity 1955 Ed. Wilson, G. S., Miles A. A. p 627 Baltimore: The Williams and Wilkins Co;
    [Google Scholar]
  27. Vischer E., Chargaff E. 1948; The separation and quantitative estimation of purines and pyrimidines in minute amounts.. J. biol. Chem. 176:703
    [Google Scholar]
  28. Wyatt G. R. 1951; The purine and pyrimidine composition of deoxypentose-nucleic acids.. Biochem. J. 48:584
    [Google Scholar]
  29. Wyatt G. R., Cohen S. S. 1953; The bases of the nucleic acids of some bacterial and animal viruses: the occurrence of 5-hydroxymethylcytosine.. Biochem. J. 55:774
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-26-2-303
Loading
/content/journal/micro/10.1099/00221287-26-2-303
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error