1887

Abstract

SUMMARY: The growth characteristics, accumulations and genetic tests carried out by means of complete transduction for over 200 independently isolated histidine-requiring () mutants of are described. Sites of mutation engendering the histidine-requiring phenotype all lie within a short chromosomal region. This region is divisible into seven smaller regions, or gene loci, each correlated with a specific physiological phenotype. The gene loci appear to be linearly arranged and are linked in the order and . The five central loci are linked in an order corresponding to the sequence of reactions in the biosynthetic pathway for histidine. The functions of the two terminal loci, and , are unknown. The loci are nearly, or truly adjacent. A few of the sites of mutation are accurately mapped within a single gene. All the evidence is consistent with the view that processes involved in intra- and inter-genic recombination are identical. Some characteristics of multisite mutations are described. Several multisite mutations are interpreted as due to deletion of genetic material while one (-57) is interpreted as due to an inversion, with or without concomitant deletion of an adjacent region. Single-site and multisite mutations exert specific effects on recombination frequencies in nearby regions.

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1960-04-01
2021-10-24
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References

  1. Abderhalden E., Weil A. 1912; Spaltung des racemischen Histidins in seine optisch aktiven Komponenten. Hoppe-Seyl. Z 77:441
    [Google Scholar]
  2. Ames B. N. 1957; The biosynthesis of histidine: l-histidinol phosphate phosphatase. J. biol. Chem 226:583
    [Google Scholar]
  3. Ames B. N., Garry B. 1959; Co-ordinate repression of the synthesis of fourhistidine biosynthetic enzymes by histidine. Proc. nat. Acad. Sci., Wash 45:1453
    [Google Scholar]
  4. Ames B. N., Garry B., Herzenberg L. 1960; .The genetic control of the enzymes of histidine biosynthesis in Salmonella typhimurium . J. gen. Microbiol 22:369
    [Google Scholar]
  5. Ames B. N., Mitchell H. K. 1952; The paper chromatography of imidazoles. J. Amer. chem. Soc 74:252
    [Google Scholar]
  6. Ames B. N., Mitchell H. K. 1955; The biosynthesis of histidine.Imidazole- glycerol phosphate, imidazoleacetol phosphate and histidinol phosphate. J. biol. Chem 212:687
    [Google Scholar]
  7. Bratton A. C., Marshall E. K. Jun 1939; A new coupling component for sulfanilamide determination. J. biol. Chem 128:537
    [Google Scholar]
  8. Chase M., Doermann A. H. 1958; High negative interference over short segments of the genetic structure of bacteriophage T4. Genetics 43:332
    [Google Scholar]
  9. Clowes R. C. 1958; Investigation of the genetics of cysteineless mutants of Salmonella typhimurium by transduction. J. gen. Microbiol 18:154
    [Google Scholar]
  10. Davis B. D. 1952; Aromatic biosynthesis. V. Antagonism between shikimic acid and its precursor, 5-dehydroshikimic acid. J. Bact 64:749
    [Google Scholar]
  11. Demerec M. 1956a; Terminology and nomenclature. Genetic Studies icith Bacteria. Publ. Carneg. Instn612 p. 104
    [Google Scholar]
  12. Demerec M. 1956b; A comparative study of certain gene loci in Salmonella . Cold Spr. Harb. Symp. quant. Biol 21:113
    [Google Scholar]
  13. Demerec M., Goldman I., Lahr E. L. 1958; Genetic recombination by transduction in Salmonella. Cold Spr. Harb. Symp. quant. Biol 23:59
    [Google Scholar]
  14. Demerec M., Hartman P. E. 1959; Complex loci in microorganisms. Anna. Rev. Microbiol 13:377
    [Google Scholar]
  15. Demerec M., Lahr E. L., Miyake T., Goldman I., Balbinder E., Banic S., Hashimoto K., Glanville E. V., Gross J. D. 1958; Bacterial genetics. Yearb. Carneg. Instn 57:390
    [Google Scholar]
  16. Gall J. G. 1958; Chromosomal differentiation. In The Chemical Basis of Development McElroy W. D., Glass B. ed pp. 103–35 Baltimore: The Johns Hopkins Press;
    [Google Scholar]
  17. Garen A., Zinder N. D. 1955; Radiological evidence for partial genetic homology between bacteriophage and host bacteria. Virology 1:347
    [Google Scholar]
  18. Hartman P. E. 1956; Linked loci in the control of consecutive steps in the primary pathway of histidine synthesis in Salmonella typhimurium . Genetic Studies with Bacteria, Publ. Carneg.Instn612 p. 35
    [Google Scholar]
  19. Hartman P. E. 1957; Transduction: A comparative review. In The Chemical Basis of Heredity McElroy W. D., Glass B. ed pp. 408–462 Baltimore: The Johns Hopkins Press;
    [Google Scholar]
  20. Hartman P. E., Goodgal S. H. 1959; Bacterial genetics (with particular reference to genetic transfer). Annu. Rev. Microbiol 13:465
    [Google Scholar]
  21. Hartman P. E., Hartman Z., Serman D. 1960; Complementation mapping by abortive transduction of histidine-requiring Salmonella mutants. J. gen. Microbiol 22:354
    [Google Scholar]
  22. Hershey A. D., Chase M. 1952; Independent functions of viral protein and nucleic acid in growth of bacteriophage. J. gen. Physiol 36:39
    [Google Scholar]
  23. Hotchkiss R. D., Evans A. H. 1958; Analysis of the complex sulfonamide resistance locus of Pneumococcus . Cold Spr. Harb. Symp. quant. Biol 23:85
    [Google Scholar]
  24. Kaudewitz F., Vielmetter W., Friedrich-Freksa H. 1958; Mutagene Wirkung des Zerfalles von radioaktivem Phosphor nachEinbau in Zellen von Escherichia coli . Z. Naturf 13b:793
    [Google Scholar]
  25. Lederberg J. 1950; Isolation and characterization of biochemical mutants of bacteria. Meth. Med. Res 3:5
    [Google Scholar]
  26. Lederberg J. 1955; Recombination mechanisms in bacteria. J. cell. comp. Physiol 45:Suppl 275
    [Google Scholar]
  27. Lederberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants. J. Bact 63:399
    [Google Scholar]
  28. Lilleengen K. 1948; Typing Salmonella typhimurium by means of bacteriophage. Acta path, microbiol. scand Suppl 77:68–75
    [Google Scholar]
  29. Magasanik B. 1958; The metabolic regulation of purine interconversions and of histidine biosynthesis. In The Chemical Basis of Development McElroy W. D., Glass B. ed p. 485 Baltimore: The Johns Hopkins Press;
    [Google Scholar]
  30. Mitchell M. B., Mitchell H. K. 1950; The selective advantage of an adenineless double mutant over one of the single mutants involved. Proc. nat. Acad. Sci., Wash 36:115
    [Google Scholar]
  31. Moyed H. S., Friedman M. 1959; Interference with feedback control: a mechanism of antimetabolite action. Science 129:986
    [Google Scholar]
  32. Moyed H. S., Magasanik B. 1957; The role of purines in histidine biosynthesis. J. Amer. chem. Soc 79:4812
    [Google Scholar]
  33. Moyed H. S., Magasanik B. 1958; Biosynthesis of the imidazole ring of histidine: genetic and environmental control. Abstr. Commun. 7th Int. Congr. Microbiol p. 141
    [Google Scholar]
  34. Nomenclature 1958 Microbial Genetics Bull 1638
    [Google Scholar]
  35. Ozeki H. 1959; Chromosome fragments participating in transduction in Salmonella typhimurium . Genetics 44:457
    [Google Scholar]
  36. Ryan F. J., Schneider L. K. 1949; The consequences of mutation during the growth of biochemical mutants of Escherichia coli. IV. The mechanism of inhibition of histidine-independent bacteria by histidineless bacteria. J. Bact 58:201
    [Google Scholar]
  37. Stokes J. L., Bayne H. G. 1958; Growth-factor-dependent strains of Salmonellae. J. Bact 76:417
    [Google Scholar]
  38. Vogel H. J., Bonner D. M. 1956; Acetylornithinase of Escherichia coli: partial purification and some properties. J. biol. Chem 218:97
    [Google Scholar]
  39. Vogel H. J., Davis B. D., Mingioli E. S. 1951; l-Histidinol, a precursor of l-histidine in Escherichia coli . J. Amer. chem. Soc 73:1897
    [Google Scholar]
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