1887

Abstract

Summary: Preparations made by treating tobacco rattle virus with phenol were about 5% as infective as the initial virus suspensions when assayed on French bean, but less on tobacco. Virus nucleic acid seems to be a major constituent of such preparations. By constrast with whole virus, nucleic acid preparations lost infectivity when incubated for 20 min. with 0·02 mg. ribonuclease/l. or when stored for a day at 20°. Nucleic acid preparations contain threads about 1 m in diameter but very few or no virus rods. The infectivity of nucleic acid preparations was little affected by highspeed centrifugation for periods in which more than 95 % of whole virus was sedimented. Ultraviolet-irradiated nucleic acid preparations were photoreactivable although irradiated preparations of whole virus were not. Nucleic acid preparations were only infective when made from purified virus suspensions or from frozen and clarified saps when these contained infective rod-shaped particles 179–192 m long. Phenol treatment of purified non-infective particles 73–77 m long yielded noninfective preparations.

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/content/journal/micro/10.1099/00221287-21-3-591
1959-12-01
2021-08-04
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References

  1. Bawden F. C., Kleczkowski A. 1955; Studies on the ability of light to counteract the inactivating action of ultraviolet radiation on plant viruses. J. gen. Microbiol 13:370
    [Google Scholar]
  2. Bawden F. C., Kleczkowski A. 1959a; .Some properties of decomposition products of potato virus. X. Virology 7:375
    [Google Scholar]
  3. Bawden F. C., Kleczkowski A. 1959b; Photoreactivation of nucleic acid from tobacco mosaic virus. Nature; London: 183503
    [Google Scholar]
  4. Bawden F. C., Pirie N. W. 1957; The activity of fragmented and reassembled tobacco mosaic virus. J. gen. Microbiol 17:80
    [Google Scholar]
  5. Cadman C. H., Harrison B. D. 1959; Studies on the properties of soil-borne viruses of the tobacco-rattle type occurring in Scotland. Ann. appl. Biol 47:542
    [Google Scholar]
  6. Commoner B. 1957; The biological activity of tobacco mosaic virus components. In Cellular Biology, Nucleic acids, and Viruses New York: New York Academy of Sciences;
    [Google Scholar]
  7. Fraenkel-Conrat H. 1956; The role of the nucleic acid in the reconstitution of active tobacco mosaic virus. J. Amer. chem. Soc 78:882
    [Google Scholar]
  8. Fraenkel-Conrat H. 1957; The infectivity of tobacco mosaic virus nucleic acid. In Cellular Biology, Nucleic acids, and Viruses New York; New York Academy of Sciences:
    [Google Scholar]
  9. Fraenkel-Conrat H., Singer B. 1957; Virus reconstitution. II. Combination of protein and nucleic acid from different strains. Biochim. biophys. Acta 24:540
    [Google Scholar]
  10. Fraenkel-Conrat H., Singer B., Williams R. C. 1957; Infectivity of viral nucleic acid. Biochim. biophys. Acta 25:87
    [Google Scholar]
  11. Gierer A., Schramm G. 1956; Die Infektionsität der Nucleinsäure aus Tabak-mosaikvirus. Z. Naturf 11b 138
    [Google Scholar]
  12. Hall C. E. 1956; Method for the observation of macromolecules with the electron microscope illustrated with micrographs of DNA. J. biophys. biochem. Cytol 2:625
    [Google Scholar]
  13. Harrison B. D., Nixon H. L. 1959; Separation and properties of particles of tobacco rattle virus with different lengths. J. gen. Microbiol 21:569
    [Google Scholar]
  14. Hart R. G. 1958; The nucleic acid fiber of the tobacco mosaic virus particle. Biochim. biophys. Acta 28:457
    [Google Scholar]
  15. Kaper J. M., Steere R. L. 1959; Infectivity of tobacco ringspot virus nucleic acid preparations. Virology 7:127
    [Google Scholar]
  16. Nixon H. L., Harrison B. D. 1959; Electron microscopic evidence on the structure of the particles of tobacco rattle virus. J. gen. Microbiol 21:582
    [Google Scholar]
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