1887

Abstract

SUMMARY: Cultures of 351 miscellaneous Gram-negative rods including 54 pseudomonads were tested for their capacity to break down arginine rapidly by Møller’s qualitative technique, by a new qualitative method, and by a quantitative technique which uses standardized washed suspensions. All but 5 of the pseudomonads gave positive results by both qualitative techniques. Among the other organisms tested, positive results were given by Aeromonas strains, some Cloaca strains, some Salmonellas, and, in the case of the new qualitative technique, by 5 strains among other groups. With the quantitative technique 50 % of the arginine was broken down in 2 hr. by all but one of the pseudomonads as compared with only four strains among the other groups. The arginine was broken down completely by 49 of the 54 pseudomonads, but by no other organisms. The ability to break down arginine rapidly is a useful taxonomic feature for pseudomonads and the qualitative tests may have practical value in the identification of strains which do not produce pigment.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-21-2-389
1959-10-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/21/2/mic-21-2-389.html?itemId=/content/journal/micro/10.1099/00221287-21-2-389&mimeType=html&fmt=ahah

References

  1. Bergey’s Manual of Determinative Bacteriology 1948, 6th ed. Breed R. S., Murray E. G. D., Hitchens A. P. Edited by London: Baillére, Tindall and Cox;
  2. Christensen W. B. 1946; Urea decomposition as a means of differentiating Proteus and Paracolon cultures from each other and from Salmonella and Shigella types. J. Bact 52:461
    [Google Scholar]
  3. Gaby W. L., Hadley C. 1957; Practical laboratory test for the identification of Pseudomonas aeruginosa . J. Bact 74:356
    [Google Scholar]
  4. Gordon J., McLeod J. W. 1928; The practical application of the direct oxidase reaction in bacteriology. J. Path. Bact 31:185
    [Google Scholar]
  5. Hills G. M. 1940; Ammonia production by pathogenic bacteria. Biochem. J 34:1057
    [Google Scholar]
  6. Kluyver A. J., van Niel C. B. 1936; Prospects for a natural system of classification of bacteria. Zbl. Bakt 2 Abt. 94:369
    [Google Scholar]
  7. Knivett V. A. 1952; Citrulline as an intermediate in the breakdown of arginine by Streptococcus faecalis . Biochem. J 50:xxx
    [Google Scholar]
  8. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature; Lond: 178703
    [Google Scholar]
  9. Miles E. M., Miles A. A. 1951; The identity of Proteus hydrophilus Bergey et al. and Proteus melanovogenes Miles & Hainan, and their relation to the genus Aeromonas Kluyver & van Niel. J. gen. Microbiol 5:298
    [Google Scholar]
  10. Møller V. 1955; Simplified tests for some amino acid decarboxylases and for the arginine dihydrolase system. Acta path, microbiol. scand 36:158
    [Google Scholar]
  11. Nyberg C. 1935; Bacillus faecalis alcaligenes Petruschky. Zbl. Bakt 1 Abt 133:443
    [Google Scholar]
  12. Rosenberg H., Ennor A. H., Morrison J. F. 1956; The estimation of arginine. Biochem. J 63:158
    [Google Scholar]
  13. Sherris J. C., Preston N. W., Shoesmith J. G. 1957; The influence of oxygen and arginine on the motility of a strain of Pseudomonas sp. J. gen, Microbiol 16:86
    [Google Scholar]
  14. Slade H. D., Doughty C. C., Slamp W. C. 1954; The synthesis of high-energy phosphate in the citrulline ureidase reaction by soluble enzymes of Pseudomonas . Arch. Biochem. Biophys 48:388
    [Google Scholar]
  15. Slade H. D., Slamp W. C. 1952; The formation of arginine dihydrolase by streptococci and some properties of the enzyme system. J. Bact 64:455
    [Google Scholar]
  16. Stanier R. Y. 1943; A note on the taxonomy of Proteus hydrophilus . J. Bact 46:213
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-21-2-389
Loading
/content/journal/micro/10.1099/00221287-21-2-389
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error