1887

Abstract

Several independent gene clusters containing varying lengths of type I polyketide synthase genes were isolated from NS3226, a producer of nanchangmycin and meilingmycin. The former is a polyether compound similar to dianemycin and the latter is a macrolide compound similar to milbemycin, which shares the same macrolide ring as avermectin but has different side groups. Clusters A–H spanned about 133, 132, 104, 174, 122, 54, 37 and 59 kb, respectively. Two systems were developed for functional analysis of the gene clusters by gene disruption or replacement. (1) phage ϕC31 and its derived vectors can infect and lysogenize this strain. (2) pSET152, an plasmid with ϕC31 site, and pHZ1358, a shuttle cosmid vector, both carrying from RP4, can be mobilized from into NS3226 by conjugation. pHZ1358 was shown to be generally useful for generating mutant strains by gene disruption and replacement in NS3226 as well as in several other strains. A region in cluster A (∼133 kb) seemed to be involved in nanchangmycin production because replacement of several DNA fragments in this region by an apramycin resistance gene [] gave rise to nanchangmycin non-producing mutants.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-148-2-361
2002-02-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/148/2/1480361a.html?itemId=/content/journal/micro/10.1099/00221287-148-2-361&mimeType=html&fmt=ahah

References

  1. Bailey, C. R., Bruton, C. J., Butler, M. J., Chater, K. F., Harris, J. E. & Hopwood, D. A. ( 1986; ). Properties of in vitro recombinant derivatives of pJV1, a multi-copy plasmid from Streptomyces phaeochromogenes. J Gen Microbiol 132, 2071-2078.
    [Google Scholar]
  2. Bevitt, D. J., Cortes, J., Haydock, S. F. & Leadlay, P. F. ( 1992; ). 6-Deoxyerythronolide-B synthase 2 from Saccharopolyspora erythraea: cloning of the structural gene, sequence analysis and inferred domain structure of the multifunctional enzyme. Eur J Biochem 204, 39-49.[CrossRef]
    [Google Scholar]
  3. Bierman, M., Logan, R., O’Brien, K., Seno, E. T., Rao, R. N. & Schoner, B. E. ( 1992; ). Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116, 43-49.[CrossRef]
    [Google Scholar]
  4. Borck, K., Beggs, J. D., Brammer, W. J., Hopkens, A. S. & Murray, N. E. ( 1976; ). The construction in vitro of transducing derivatives of phage lambda. Mol Gen Genet 146, 199-207.[CrossRef]
    [Google Scholar]
  5. Burg, R. W., Miller, B. M., Baker, E. E. & 12 other authors ( 1979; ). Avermectin, new family of potent anthelmintic agents: producing organism and fermentation. Antimicrob Agents Chemother 15, 361–367.[CrossRef]
    [Google Scholar]
  6. Cane, D. E., Walsh, C. T. & Khosla, C. ( 1998; ). Harnessing the biosynthetic code: combinations, permutations, and mutations. Science 282, 63-68.[CrossRef]
    [Google Scholar]
  7. Cortes, J., Haydock, S. F., Roberts, G. A., Bevitt, D. J. & Leadlay, P. F. ( 1990; ). An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea. Nature 348, 176-178.[CrossRef]
    [Google Scholar]
  8. Czerwinski, E. W. & Steinrauf, L. K. ( 1971; ). Structure of the antibiotic dianemycin. Biochem Biophys Res Commun 45, 1284-1287.[CrossRef]
    [Google Scholar]
  9. Deng, Z. X., Kieser, T. & Hopwood, D. A. ( 1988; ). ‘Strong incompatibility’ between derivatives of the Streptomyces multi-copy plasmid pIJ101. Mol Gen Genet 214, 286-294.[CrossRef]
    [Google Scholar]
  10. Donadio, S. & Katz, L. ( 1992; ). Organization of the enzymatic domains in the multifunctional polyketide synthase involved in erythromycin formation in Saccharopolyspora erythraea. Gene 111, 51-60.[CrossRef]
    [Google Scholar]
  11. Donadio, S., Staver, M. J., McAlpine, J. B., Swanson, S. J. & Katz, L. ( 1991; ). Modular organization of genes required for complex polyketide biosynthesis. Science 252, 675-679.[CrossRef]
    [Google Scholar]
  12. Evans, G. A., Lewis, K. & Rothenberg, B. E. ( 1989; ). High efficiency vectors for cosmid microcloning and genomic analysis. Gene 79, 9-20.[CrossRef]
    [Google Scholar]
  13. Hanahan, D. ( 1983; ). Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166, 557-580.[CrossRef]
    [Google Scholar]
  14. Hopwood, D. A. ( 1997; ). Genetic contributions to understanding polyketide synthases. Chem Rev 97, 2465-2497.[CrossRef]
    [Google Scholar]
  15. Hopwood, D. A., Bibb, M. J., Chater, K. F. & 7 other authors/editors (1985). Genetic Manipulation of Streptomyces: a Laboratory Manual. Norwich: John Innes Foundation.
  16. Hu, Z., Bao, K., Zhou, X., Zhou, Q., Hopwood, D. A., Kieser, T. & Deng, Z. ( 1994; ). Repeated polyketide synthase modules involved in the biosynthesis of a heptaene macrolide by Streptomyces sp. FR-008. Mol Microbiol 14, 163-172.[CrossRef]
    [Google Scholar]
  17. Hutchinson, C. R. ( 1998; ). Combinatorial biosynthesis for new drug discovery. Curr Opin Microbiol 1, 319-329.[CrossRef]
    [Google Scholar]
  18. Ikeda, H. & Omura, S. ( 1995; ). Control of avermectin biosynthesis in Streptomyces avermitilis for the selective production of a useful component. J Antibiot 48, 549-562.[CrossRef]
    [Google Scholar]
  19. Ikeda, H., Nonomiya, T., Usami, M., Ohta, T. & Omura, S. ( 1999; ). Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. Proc Natl Acad Sci USA 96, 9509-9514.[CrossRef]
    [Google Scholar]
  20. Janssen, G. R. & Bibb, M. J. ( 1993; ). Derivatives of pUC18 that have BglII sites flanking a modified multiple cloning site and that retain the ability to identify recombinant clones by visual screening of Escherichia coli colonies. Gene 124, 133-134.[CrossRef]
    [Google Scholar]
  21. Katz, L. ( 1997; ). Manipulation of modular polyketide synthases. Chem Rev 97, 2557-2576.[CrossRef]
    [Google Scholar]
  22. Katz, L. & McDaniel, R. ( 1999; ). Novel macrolides through genetic engineering. Med Res Rev 19, 543-558.[CrossRef]
    [Google Scholar]
  23. Katz, E., Thompson, C. J. & Hopwood, D. A. ( 1983; ). Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans 66. J Gen Microbiol 129, 2703-2714.
    [Google Scholar]
  24. Kieser, T., Hopwood, D. A., Wright, H. M. & Thompson, C. J. ( 1982; ). pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors. Mol Gen Genet 185, 223-238.[CrossRef]
    [Google Scholar]
  25. Kieser, T., Bibb, M. J., Buttner, M. J., Chater, K. F. & Hopwood, D. A. (2000). Practical Streptomyces Genetics. Norwich: John Innes Foundation.
  26. Leadlay, P. F. ( 1997; ). Combinatorial approaches to polyketide biosynthesis. Curr Opin Chem Biol 1, 162-168.[CrossRef]
    [Google Scholar]
  27. Lomovskaya, N. D., Mkrtumian, N. M., Gostimskaya, N. L. & Danilenko, V. N. ( 1972; ). Characterization of temperate actinophage ϕC31 isolated from Streptomyces coelicolor A3(2). J Virol 9, 258-262.
    [Google Scholar]
  28. Lydiate, D. J., Malpartida, F. & Hopwood, D. A. ( 1985; ). The Streptomyces plasmid SCP2*: its functional analysis and development into useful cloning vectors. Gene 35, 223-235.[CrossRef]
    [Google Scholar]
  29. MacNeil, D. J., Gewain, K. M., Ruby, C. L., Dezeny, G., Gibbons, P. H. & MacNeil, T. ( 1992; ). Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector. Gene 111, 61-68.[CrossRef]
    [Google Scholar]
  30. Oh, S.-H. & Chater, K. F. ( 1997; ). Denaturation of circular or linear DNA facilitates targeted integrative transformation of Streptomyces coelicolor A3(2): possible relevance to other organisms. J Bacteriol 179, 122-127.
    [Google Scholar]
  31. Ouyang, L., Wan, S., Tu, G., Chen, X., Zhen, Y. & Gao, Y. ( 1984; ). A new species of Streptomyces producing insecticidal antibiotics. Chin J Microbiol 24, 195-199.
    [Google Scholar]
  32. Ouyang, L., Tu, G., Gao, Y., Zhang, P. & Xie, X. ( 1993; ). Two insecticidal antibiotics produced by Streptomyces nanchangensis. J Jiangxi Agricul Univ 15, 148-153.
    [Google Scholar]
  33. Pigac, J., Vujaklija, D., Toman, Z., Gamulin, V. & Schrempf, H. ( 1988; ). Structural instability of a bifunctional plasmid pZG1 and single-stranded DNA formation in Streptomyces. Plasmid 19, 222-230.[CrossRef]
    [Google Scholar]
  34. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  35. Santi, D. V., Siani, M. A., Julien, B., Kupfer, D. & Roe, B. ( 2000; ). An approach for obtaining perfect hybridization probes for unknown polyketide synthase genes: a search for the epothilone gene cluster. Gene 247, 97-102.[CrossRef]
    [Google Scholar]
  36. Schwecke, T., Aparicio, J. F., Molnar, I. & 10 other authors ( 1995; ). The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin. Proc Natl Acad Sci USA 92, 7839–7843.[CrossRef]
    [Google Scholar]
  37. Takahashi, S., Miyaoka, H., Tanaka, K., Enoktta, R. & Okazaki, T. ( 1993; ). Milbemycin a11, a12, a13, a14, and a15, a new family of milbemycins from Streptomyces hygroscopicus ssp. aureolacrimosus, taxonomy, fermentation, isolation, structure elucidation and biological properties. J Antibiot 49, 1364-1371.
    [Google Scholar]
  38. Thompson, C. J., Kieser, T., Ward, J. M. & Hopwood, D. A. ( 1982; ). Physical analysis of antibiotic-resistant genes from Streptomyces and their use in vector construction. Gene 20, 51-62.[CrossRef]
    [Google Scholar]
  39. Tsai, J. F.-Y. & Chen, C. W. ( 1987; ). Isolation and characterization of Streptomyces lividans mutants deficient in intraplasmid recombination. Mol Gen Genet 208, 211-218.[CrossRef]
    [Google Scholar]
  40. Ward, J. M., Janssen, G. R., Kieser, T. & Bibb, M. J. ( 1986; ). Construction and characterization of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator. Mol Gen Genet 203, 468-478.[CrossRef]
    [Google Scholar]
  41. Weber, J. M., Leung, J. O., Maine, G. T., Potenz, R. H., Paulus, T. J. & DeWitt, J. P. ( 1990; ). Organization of a cluster of erythromycin genes in Saccharopolyspora erythraea. J Bacteriol 172, 2372-2383.
    [Google Scholar]
  42. Xue, Y., Zhao, L., Liu, H.-W. & Sherman, D. H. ( 1998; ). A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. Proc Natl Acad Sci USA 95, 12111-12116.[CrossRef]
    [Google Scholar]
  43. Zaman, S., Radnedge, L., Richards, H. & Ward, J. M. ( 1993; ). Analysis of the site for second-strand initiation during replication of the Streptomyces plasmid pIJ101. J Gen Microbiol 139, 669-676.[CrossRef]
    [Google Scholar]
  44. Zhou, X., Deng, Z., Hopwood, D. A. & Kieser, T. ( 1994; ). Characterization of HAU3, a broad-host-range temperate Streptomyces phage, and development of plasmids. J Bacteriol 176, 2096-2099.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-148-2-361
Loading
/content/journal/micro/10.1099/00221287-148-2-361
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error