1887

Abstract

The manufacture of yoghurt relies on the simultaneous utilization of two starters: and subsp. (). A protocooperation usually takes place between the two species, which often results in enhanced milk acidification and aroma formation compared to pure cultures. Cell-wall proteinases of and lactobacilli have been shown to be essential to growth in milk in pure cultures. In this study, the role of proteinases PrtS from and PrtB from in bacterial growth in milk was evaluated; a negative mutant for the gene of CNRZ 385 was constructed for this purpose. Pure cultures of CNRZ 385 and its PrtS-negative mutant were made in milk as well as mixed cultures of and : CNRZ 385 or its PrtS-negative mutant was associated with several strains of , including a PrtB-negative strain. The pH and growth of bacterial populations of the resulting mixed cultures were followed, and the strain was found to influence both the extent of the benefit of / association on milk acidification and the magnitude of population dominance at the end of fermentation. In all mixed cultures, the sequential growth of then of and finally of both bacteria was observed. Although proteinase PrtS was essential to growth in milk in pure culture, it had no effect on bacterial growth and thus on the final pH of mixed cultures in the presence of PrtB. In contrast, proteinase PrtB was necessary for the growth of , and its absence resulted in a higher final pH. From these results, a model of growth of both bacteria in mixed cultures in milk is proposed.

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2002-11-01
2020-03-29
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