1887

Abstract

A hidden Markov model of σ RNA polymerase cofactor recognition sites in , containing either the common or the extended −10 motifs, has been constructed based on experimentally verified σ recognition sites. This work suggests that more information exists at the initiation site of transcription in both types of promoters than previously thought. When tested on the entire genome, the model predicts that approximately half of the σ recognition sites are of the extended type. Some of the response-regulator aspartate phosphatases were among the predictions of promoters containing extended sites. The expression of and was confirmed by site-directed mutagenesis to depend on the extended −10 region.

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2001-09-01
2019-10-17
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