1887

Abstract

Expression of a gene encoding a novel protein antigen of 40 kDa (p40) was detected in IS strains of , but not in any other species or subspecies of tested, including IS and the other members of the complex. Although Southern hybridization revealed that the p40 gene is widely distributed within the genus, expression of the antigen could not be detected on Western blots of mycobacterial cell lysates. Nucleotide sequence analysis of the cloned p40 gene, and a database search, revealed high levels of sequence identity with a homologous gene in IS , subsp. , , , and . Further analysis of upstream sequences identified a putative promoter region. The p40 gene is the first example of a gene that is widely distributed within the genus but expressed only in association with the presence of a genomic insertion element, in this case IS, in strains of isolated from birds and domestic livestock.

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2001-06-01
2021-10-22
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