1887

Abstract

The and genes, encoding the large (α) and small (β) subunit of glutamate synthase (GOGAT), were investigated in this study. Using RT-PCR, a common transcript of and was shown. Reporter gene assays and Northern hybridization experiments revealed that transcription of this operon depends on nitrogen starvation. The expression of is under control of the global repressor protein AmtR as demonstrated by gel shift experiments and analysis of transcription in an deletion strain. In contrast to other bacteria, in GOGAT plays no pivotal role; e.g. and inactivation did not result in growth defects when cells were grown in standard minimal medium and only a slight increase in the doubling time of the corresponding mutant strains was observed in the presence of limiting amounts of ammonia or urea. Additionally, mutant analyses revealed that GOGAT has no essential function in glutamate production by .

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2001-11-01
2019-12-13
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