1887

Abstract

sp. strain KR20, which was isolated from a polycyclic aromatic hydrocarbon (PAH) contaminated soil of a former gaswork plant site, metabolized about 60% of the fluoranthene added (05 mg ml) to batch cultures in mineral salts medium within 10 d at 20 °C. It thereby increased its cell number about 30-fold and produced at least seven metabolites. Five metabolites, namely -2,3-fluoranthene dihydrodiol, -9-carboxymethylene-fluorene-1-carboxylic acid, -1,9a-dihydroxy-1-hydro-fluorene-9-one-8-carboxylic acid, 4-hydroxybenzochromene-6-one-7-carboxylic acid and benzene-1,2,3-tricarboxylic acid, could be identified by NMR and MS spectroscopic techniques and ascribed to an alternative fluoranthene degradation pathway. Besides fluoranthene, the isolate could not use any of the PAHs tested as a sole source of carbon and energy.

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2001-10-01
2020-03-29
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References

  1. Babson J. R., Russo-Rodruiguez S. E., Wattley R. V., Bergstein P. L., Rastetter W. H., Liber H. L., Andon B. M., Thilly W. G., Wogan G. N.. 1986; Microsomal activation of fluoranthene to mutagenic metabolites. Toxicol Appl Pharm85:355–366[CrossRef]
    [Google Scholar]
  2. Bastiaens L., Springael D., Wattiau P., Harms H., deWachter R., Verachtert H., Diels L.. 2000; Isolation of adherent polycyclic aromatic hydrocarbon (PAH)-degrading bacteria using PAH-sorbing carriers. Appl Environ Microbiol66:1834–1843[CrossRef]
    [Google Scholar]
  3. Boldrin B., Thiem A., Fritsche C.. 1993; Degradation of phenanthrene, fluorene, fluoranthene, and pyrene by a Mycobacterium sp. Appl Environ Microbiol59:1927–1930
    [Google Scholar]
  4. Böttger E. C., Kirschner P., Springer B., Zumft W.. 1997; Mycobacteria degrading polycyclic aromatic hydrocarbons. Int J Syst Bacteriol47:247[CrossRef]
    [Google Scholar]
  5. Bouchez M., Blanchet D., Vandecasteele J.-P.. 1995; Degradation of polycyclic aromatic hydrocarbons by pure strains and by defined strain associations: inhibition phenomena and cometabolism. Appl Microbiol Biotechnol43:156–164[CrossRef]
    [Google Scholar]
  6. Bressler D. C., Fedorak P. M.. 2000; Bacterial metabolism of fluorene, dibenzofurane, dibenzothiophene, and carbazole. Can J Microbiol46:397–403[CrossRef]
    [Google Scholar]
  7. Bryniok D.. 1994; PAK-Abbau in Mehrphasensystemen. . In Biologischer Abbau von polycyclischen aromatischen Kohlenwasserstoffen pp91–108 Edited by Weigert B.. Berlin: SFB 193 der TU Berlin;
    [Google Scholar]
  8. Dagher F., Deziel E., Lirette P., Paquette G., Bisaillon J.-G., Villemur R.. 1997; Comparative study of five polycyclic aromatic hydrocarbon degrading bacterial strains isolated from contaminated soils. Can J Microbiol46:368–377
    [Google Scholar]
  9. Dean-Ross D., Cerniglia C. E.. 1996; Degradation of pyrene by Mycobacterium flavescens . Appl Microbiol Biotechnol46:307–312[CrossRef]
    [Google Scholar]
  10. Edwards N. T.. 1983; Polycyclic aromatic hydrocarbons (PAH’s) in the terrestial environment – a review. J Environ Qual12:427–441
    [Google Scholar]
  11. Fernley H. N., Griffiths E., Evans W. C.. 1964; Oxidative metabolism of phenanthrene and anthracene by soil bacteria: the initial ring-fission step. Biochem J91:15p–16p
    [Google Scholar]
  12. Grifoll M., Selifonov S. A., Chapman P. J.. 1994; Evidence for a novel pathway in the degradation of fluorene by Pseudomonas sp. strain F274. Appl Environ Microbiol60:2438–2449
    [Google Scholar]
  13. Harvey R. G.. 1991; Polycyclic Aromatic Hydrocarbons: Chemistry and Carcinogenicity New York, Port Chester, Melbourne, Sydney: Cambridge University Press;
    [Google Scholar]
  14. Heitkamp M. A., Franklin W., Cerniglia C. E.. 1988; Microbial metabolism of polycyclic aromatic hydrocarbons: isolation and characterization of a pyrene-degrading bacterium. Appl Environ Microbiol54:2549–2555
    [Google Scholar]
  15. Higson F.. 1992; Microbial degradation of biphenyl and its derivatives. Adv Appl Microbiol37:135–164
    [Google Scholar]
  16. Ho Y., Jackson M., Yang Y., Mueller J. G., Pritchard P. H.. 2000; Characterization of fluoranthene- and pyrene-degrading bacteria isolated from PAH-contaminated soils and sediments. J Ind Microbiol Biotechnol24:100–112[CrossRef]
    [Google Scholar]
  17. Jeffrey A. M., Yeh H. J. C., Jerina D. M., Patel T. R., Davey J. F., Gibson D. T.. 1975; Initial reactions in the oxidation of naphthalene by Pseudomonas putida . Biochemistry14:575–584[CrossRef]
    [Google Scholar]
  18. Jerina D. M., Selander H., Yagi H., Wells M. C., Davey J. F., Mahadevan V., Gibson D. T.. 1976; Dihydrodiols from anthracene and phenanthrene. J Am Chem Soc98:5988–5996[CrossRef]
    [Google Scholar]
  19. Juhasz A. L., Britz M. L., Stanley G. A.. 1997; Degradation of fluoranthene, pyrene, benz[a]anthracene and dibenz[a,h]anthracene by Burkholderia cepacia . J Appl Microbiol83:189–198[CrossRef]
    [Google Scholar]
  20. Kanaly R. A., Harayama S.. 2000; Biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by bacteria. J Bacteriol182:2059–2067[CrossRef]
    [Google Scholar]
  21. Kästner M., Breuer-Jammali M., Mahro B.. 1994; Enumeration and characterization of the soil microflora from hydrocarbon-contaminated soil sites able to mineralize polycyclic aromatic hydrocarbons (PAH. Appl Microbiol Biotechnol41:267–273[CrossRef]
    [Google Scholar]
  22. Kelley I., Freeman J., Evans F. E., Cerniglia C. E.. 1993; Identification of metabolites from the degradation of fluoranthene by Mycobacterium sp. strain PYR-1. Appl Environ Microbiol59:800–806
    [Google Scholar]
  23. Kiyohara H., Nagao K., Yana K.. 1982; Rapid screen for bacteria degrading water-insoluble, solid hydrocarbons on agar plates. Appl Environ Microbiol43:454–457
    [Google Scholar]
  24. Kleespies M., Kroppenstedt R. M., Rainey F. A., Webb L. E., Stackebrandt E.. 1996; Mycobacterium hodleri , sp. nov., a new member of the fast-growing mycobacteria capable of degrading polycyclic aromatic hydrocarbons. Int J Syst Bacteriol46:683–687[CrossRef]
    [Google Scholar]
  25. Lloyd-Jones G., Hunter D. W. F.. 1997; Characterization of fluoranthene- and pyrene-degrading Mycobacterium -like strains by RAPD and SSU sequencing. FEMS Microbiol Lett153:51–56[CrossRef]
    [Google Scholar]
  26. Minnikin D. E., Alshamaony L., Goodfellow M.. 1975; Differentiation of Mycobacterium , Nocardia , and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol88:200–204[CrossRef]
    [Google Scholar]
  27. Mueller J. G., Chapman P. J., Blattmann B. O., Pritchard P. H.. 1990; Isolation and characterization of a fluoranthene-utilizing strain of Pseudomonas paucimobilis . Appl Environ Microbiol56:1079–1086
    [Google Scholar]
  28. Mueller J. G., Devereux R., Santavy D. L., Lantz S. E., Willis S. G., Pritchard P. H.. 1997; Phylogenetic and physiological comparison of PAH-degrading bacteria from geographically diverse soils. Antonie Leeuwenhoek71:329–343[CrossRef]
    [Google Scholar]
  29. Pothuluri J. V., Heflich R. H., Fu P. P., Cerniglia C. E.. 1992; Fungal metabolism and detoxification of fluoranthene. Appl Environ Microbiol58:937–941
    [Google Scholar]
  30. Reasoner D. J., Geldreich E. E.. 1985; A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol49:1–7
    [Google Scholar]
  31. Rehmann K., Steinberg C. E. W., Kettrup A. A.. 1996; Branched metabolic pathway for phenanthrene degradation in a pyrene-degrading bacterium. Polycycl Aromat Comp11:125–130[CrossRef]
    [Google Scholar]
  32. Rehmann K., Noll H. P., Steinberg C. E. W., Kettrup A. A.. 1998; Pyrene degradation by Mycobacterium sp . strain KR2. Chemosphere36:2977–2992[CrossRef]
    [Google Scholar]
  33. Rehmann K., Hertkorn N., Kettrup A. A.. 1999; Bacterial fluoranthene degradation – indication for a novel degradation pathway. In Novel Approaches for Bioremediation of Organic PollutionProceedings of the 42nd Oholo Conference EilatIsrael (3.5–7.5.1998). pp39–46 Edited by Fass R., Flashner Y., Reuveny S.. New York, Boston, Dordrecht, London, Moscow: Kluwer Academic/Plenum;
    [Google Scholar]
  34. Rice J. E., LaVoie E. J., Hoffmann D.. 1983; Synthesis of the isomeric phenols and the trans -2,3-dihydrodiol of fluoranthene. J Org Chem48:2360–2363[CrossRef]
    [Google Scholar]
  35. Selifonov S. A., Grifoll M., Gurst J. E., Chapman P. J.. 1993; Isolation and characterization of (+)-1,1a-dihydroxy-1-hydroxyfluoren-9-one formed by angular dioxygenation in the bacterial catabolism of fluorene. Biochem Biophys Res Commun193:67–76[CrossRef]
    [Google Scholar]
  36. Sepic E., Leskovsek H.. 1999; Isolation and identification of fluoranthene biodegradation products. Analyst124:1765–1769[CrossRef]
    [Google Scholar]
  37. Sepic E., Bricelj M., Leskovsek H.. 1998; Degradation of fluoranthene by Pasteurella sp. IFA and Mycobacterium sp. PYR1: isolation and identification of metabolites. J Appl Microbiol85:746–754[CrossRef]
    [Google Scholar]
  38. Suess M. J.. 1976; The environmental load and cycle of polycyclic aromatic hydrocarbons. Sci Total Environ6:239–250[CrossRef]
    [Google Scholar]
  39. Sutherland J. B., Rafii F., Khan A. A., Cerniglia C. E.. 1995; Mechanisms of polycyclic aromatic hydrocarbon degradation. In Microbial Transformation and Degradation of Toxic Organic Chemicals pp269–306 Edited by Young L. Y., Cerniglia C. E.. New York, Chichester, Brisbane, Toronto, Singapore: Wiley-Liss;
    [Google Scholar]
  40. Thibault S. L., Anderson M., Frankenberger W. T. Jr. 1996; Influence of surfactants on pyrene desorption and degradation in soils. Appl Environ Microbiol61:283–287
    [Google Scholar]
  41. Thomas A. O., Lester J. N.. 1993; The microbial remediation of former gasworks sites: a review. Environ Technol14:1–24[CrossRef]
    [Google Scholar]
  42. Thomas A. O., Lester J. N.. 1994; The reclamation of disused gaswork sites: new solutions to an old problem. Sci Total Environ152:239–260[CrossRef]
    [Google Scholar]
  43. Walter U., Beyer M., Klein J., Rehm H.-J.. 1991; Degradation of pyrene by Rhodococcus sp. UW1. Appl Microbiol Biotechnol34:671–676[CrossRef]
    [Google Scholar]
  44. Wayne L. G., Engbœk C., Engel H. B. W.. 17 other authors 1974; Highly reproducible techniques for use in systematic bacteriology in the genus Mycobacterium : tests for pigment, urease, resistance to sodium chloride, hydrolysis of Tween 80, and β-galactosidase. Int J Syst Bacteriol24:412–419[CrossRef]
    [Google Scholar]
  45. Weißenfels W. D., Beyer M., Klein J.. 1990; Degradation of phenanthrene, fluorene and fluoranthene by pure bacterial cultures. Appl Microbiol Biotechnol32:479–484[CrossRef]
    [Google Scholar]
  46. Willumsen P. A., Karlson U., Prichard P. H.. 1998; Response of fluoranthene-degrading bacteria to surfactants. Appl Microbiol Biotechnol50:475–483[CrossRef]
    [Google Scholar]
  47. Zink G., Lorber K. E.. 1995; Mass spectral identification of metabolites formed by microbial degradation of polycyclic aromatic hydrocarbons (PAH. Chemosphere31:4077–4084[CrossRef]
    [Google Scholar]
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