1887

Abstract

A cloned 2·5 kb DNA fragment that can restore antibiotic production and sporulation to a mutant encodes a 113 aa protein showing similarity to a family of anti-anti-sigma factors from and ; and the deduced product of a closely spaced downstream ORF, designated ORF3, shows similarity to cognate anti-sigma factors. The homologues in regulate the activity of sporulation- and stress-response-specific sigma factors. However, there is no sigma factor gene near and ORF3. is transcribed both as a monocistronic and a polycistronic mRNA, the latter including the downstream ORF3 gene. The two transcripts were present at all time points during growth and both were upregulated when aerial mycelium and pigmented antibiotics were seen. At all time points, the monocistronic transcript was two- to threefold more abundant than the polycistronic transcript. Mapping of the mRNA 5′ ends indicated that transcription is initiated from two transcription start sites located 82 and 123 bp upstream of the translation start. A constructed null mutant had the same phenotype as previously isolated point mutations, some of which were shown to have potentially significant base changes within . When compared to the wild-type strain, the null mutant showed no differences in the levels of transcription from the two promoters. These results suggest that is not involved in autoregulation.

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2000-09-01
2020-09-29
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