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Volume 146, Issue 7

The serine-aspartate repeat (Sdr) protein family in

The GenBank accession numbers for the sequences determined in this work are AF245041 (), AF245042 () and AF245043 ().

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Abstract

can express three different cell-surface-associated proteins, designated SdrF, SdrG and SdrH, that contain serine-aspartate dipeptide repeats. Proteins SdrF and SdrG are similar in sequence and structural organization to the Sdr proteins of and comprise unique 625- and 548-residue A regions at their N termini, respectively, followed by 110–119-residue B-repeat regions and SD-repeat regions. The C termini contain LPXTG motifs and hydrophobic amino acid segments characteristic of surface proteins covalently anchored to peptidoglycan. In contrast, SdrH has a short 60-residue A region at its N terminus followed by a SD-repeat region, a unique 277-residue C region and a C-terminal hydrophobic segment. SdrH lacks a LPXTG motif. Recombinant proteins representing the A regions of SdrF, SdrG and SdrH were expressed and purified from . Antisera specific to these proteins were raised in rabbits and used to identify Sdr proteins expressed by . Only SdrF was released from lysostaphin-generated protoplasts of cells grown to late-exponential phase. SdrG and SdrH remained associated with the protoplast fraction and thus appear to be ineffectively sorted along the conventional pathway used for cell-wall-anchored proteins. In Southern hybridization analyses, the and genes were present in all 16 strains tested, whilst was present in 12 strains. Antisera from 16 patients who had recovered from infections contained antibodies that reacted with recombinant A regions of SdrG and SdrH, suggesting that these proteins can be expressed during infection.

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  • Accepted:
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  • Published Online:
Keyword(s): adhesin , GST, glutathione S-transferase , multigene family , Sdr, serine-aspartate (SD) repeat and surface protein
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2000-07-01
2024-04-26
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The serine-aspartate repeat (Sdr) protein family in Staphylococcus epidermidisThe GenBank accession numbers for the sequences determined in this work are AF245041 (sdrF), AF245042 (sdrG) and AF245043 (sdrH).
Microbiology 146, 1535 (2000); https://doi.org/10.1099/00221287-146-7-1535
/content/journal/micro/10.1099/00221287-146-7-1535
/content/journal/micro/10.1099/00221287-146-7-1535
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