1887

Abstract

can express three different cell-surface-associated proteins, designated SdrF, SdrG and SdrH, that contain serine-aspartate dipeptide repeats. Proteins SdrF and SdrG are similar in sequence and structural organization to the Sdr proteins of and comprise unique 625- and 548-residue A regions at their N termini, respectively, followed by 110–119-residue B-repeat regions and SD-repeat regions. The C termini contain LPXTG motifs and hydrophobic amino acid segments characteristic of surface proteins covalently anchored to peptidoglycan. In contrast, SdrH has a short 60-residue A region at its N terminus followed by a SD-repeat region, a unique 277-residue C region and a C-terminal hydrophobic segment. SdrH lacks a LPXTG motif. Recombinant proteins representing the A regions of SdrF, SdrG and SdrH were expressed and purified from . Antisera specific to these proteins were raised in rabbits and used to identify Sdr proteins expressed by . Only SdrF was released from lysostaphin-generated protoplasts of cells grown to late-exponential phase. SdrG and SdrH remained associated with the protoplast fraction and thus appear to be ineffectively sorted along the conventional pathway used for cell-wall-anchored proteins. In Southern hybridization analyses, the and genes were present in all 16 strains tested, whilst was present in 12 strains. Antisera from 16 patients who had recovered from infections contained antibodies that reacted with recombinant A regions of SdrG and SdrH, suggesting that these proteins can be expressed during infection.

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2000-07-01
2020-07-15
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References

  1. Casolini F., Visai L., Joh D., Conaldi P. G., Toniolo A., Höök M., Speziale P.. 1998; Antibody response to fibronectin-binding adhesin FnbpA in patients with Staphylococcus aureus infections. Infect Immun66:5433–5442
    [Google Scholar]
  2. Christensen G. D., Simpson W. A., Bisno A. L., Beachey E. H.. 1982; Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun37:318–326
    [Google Scholar]
  3. Fleer A., Verhoef J.. 1989; An evaluation of the role of surface hydrophobicity and extracellular slime in the pathogenesis of foreign-body-related infections due to coagulase-negative staphylococci. J Invest Surg2:391–396[CrossRef]
    [Google Scholar]
  4. Foster T. J., Höök M.. 1998; Surface protein adhesins of Staphylococcus aureus. Trends Microbiol6:484–488[CrossRef]
    [Google Scholar]
  5. Hartford O., Francois P., Vaudaux P., Foster T. J.. 1997; The dipeptide repeat region of the fibrinogen-binding protein (clumping factor) is required for functional expression of the fibrinogen-binding domain on the Staphylococcus aureus cell surface. Mol Microbiol25:1065–1076[CrossRef]
    [Google Scholar]
  6. Hartford O., McDevitt D., Foster T. J.. 1999; Matrix-binding proteins of Staphylococcus aureus: functional analysis of mutant and hybrid molecules. Microbiology145:2497–2505
    [Google Scholar]
  7. von Heijne G.. 1983; Patterns of amino acids near signal-sequence cleavage sites. Eur J Biochem133:17–21[CrossRef]
    [Google Scholar]
  8. Heilmann C., Schweitzer O., Gerke C., Vanittanakom N., Mack D., Götz F.. 1996; Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis. Mol Microbiol20:1083–1091[CrossRef]
    [Google Scholar]
  9. Heilmann C., Hussain M., Peters G., Gotz F.. 1997; Evidence for autolysin-mediated primary attachment of Staphylococcus epidermidis to a polystyrene surface. Mol Microbiol24:1013–1024[CrossRef]
    [Google Scholar]
  10. Herrmann M., Vaudaux P. E., Pittet D., Auckenthaler R., Lew P. D., Schumacher-Perdreau F., Peters G., Waldvogel F. A.. 1988; Fibronectin, fibrinogen, and laminin act as mediators of adherence of clinical staphylococcal isolates to foreign material. J Infect Dis158:693–701[CrossRef]
    [Google Scholar]
  11. Joh H. J., House-Pompeo K., Patti J. M., Gurusiddappa S., Höök M.. 1994; Fibronectin receptors from gram-positive bacteria: comparison of active sites. Biochemistry33:6086–6092[CrossRef]
    [Google Scholar]
  12. Josefsson E., McCrea K. W., Nı́ Eidhin D., O’Connell D., Cox J., Höök M., Foster T. J.. 1998a; Three new members of the serine-aspartate repeat protein multigene family of Staphylococcus aureus. Microbiology144:3387–3395[CrossRef]
    [Google Scholar]
  13. Josefsson E., O’Connell D., Foster T. J., Durussel I., Cox J. A.. 1998b; The binding of calcium to the B-repeat segment of SdrD, a cell surface protein of Staphylococcus aureus. J Biol Chem273:31145–31152[CrossRef]
    [Google Scholar]
  14. Kehoe M. A.. 1994; Cell-wall-associated proteins in Gram-positive bacteria. In Bacterial Cell Wall pp.217–261Edited by Ghuysen J.-M., Hakenbeck R.. Amsterdam: Elsevier Science;
    [Google Scholar]
  15. Kloos W. E., Bannerman T. L.. 1994; Update on clinical significance of coagulase-negative staphylococci. Clin Microbiol Rev7:117–140
    [Google Scholar]
  16. McCrea K. W., Watson W. J., Gilsdorf J. R., Marrs C. F.. 1997; Identification of two minor subunits in the pilus of Haemophilus influenzae. J Bacteriol179:4227–4231
    [Google Scholar]
  17. McDevitt D., Foster T. J.. 1995; Variation in the size of the repeat region of the fibrinogen receptor (clumping factor) of Staphylococcus aureus strains. Microbiology141:937–943[CrossRef]
    [Google Scholar]
  18. McDevitt D., Francois P., Vaudaux P., Foster T. J.. 1994; Molecular characterization of the clumping factor (fibrinogen receptor) of Staphylococcus aureus. Mol Microbiol11:237–248[CrossRef]
    [Google Scholar]
  19. McDevitt D., Francois P., Vaudaux P., Foster T. J.. 1995; Identification of the ligand-binding domain of the surface-located fibrinogen receptor (clumping factor) of Staphylococcus aureus. Mol Microbiol16:895–907[CrossRef]
    [Google Scholar]
  20. Mack D., Siemssen N., Laufs R.. 1992; Parallel induction by glucose of adherence and a polysaccharide antigen specific for plastic-adherent Staphylococcus epidermidis: evidence for functional relation to intercellular adhesion. Infect Immun60:2048–2057
    [Google Scholar]
  21. McKenney D., Hubner J., Muller E., Wang Y., Goldmann D. A., Pier G. B.. 1998; The ica locus of Staphylococcus epidermidis encodes production of the capsular polysaccharide/adhesin. Infect Immun66:4711–4720
    [Google Scholar]
  22. McKenney D., Pouliot K. L., Wang Y., Murthy V., Ulrich M., Doring G., Lee J. C., Goldmann D. A., Pier G. B.. 1999; Broadly protective vaccine for Staphylococcus aureus based on an in vivo-expressed antigen. Science284:1523–1527[CrossRef]
    [Google Scholar]
  23. Martin M. A., Pfaller M. A., Massanari R. M., Wenzel R. P.. 1989; Use of cellular hydrophobicity, slime production, and species identification markers for the clinical significance of coagulase-negative staphylococcal isolates. Am J Infect Control17:130–135[CrossRef]
    [Google Scholar]
  24. Nı́ Eidhin D., Perkins S., Francois P., Vaudaux P., Höök M., Foster T. J.. 1998; Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureus. Mol Microbiol30:245–257[CrossRef]
    [Google Scholar]
  25. Nilsson M., Frykberg L., Flock J. I., Pei L., Lindberg M., Guss B.. 1998; A fibrinogen-binding protein of Staphylococcus epidermidis. Infect Immun66:2666–2673
    [Google Scholar]
  26. O’Connell D. P., Nanavaty T., McDevitt D., Gurusiddappa S., Höök M., Foster T. J.. 1998; The fibrinogen-binding MSCRAMM (clumping factor) of Staphylococcus aureus has a Ca2+-dependent inhibitory site. J Biol Chem273:6821–6829[CrossRef]
    [Google Scholar]
  27. Pascual A., Fleer A., Westerdaal N. A., Verhoef J.. 1986; Modulation of adherence of coagulase-negative staphylococci to Teflon catheters in vitro. Eur J Clin Microbiol5:518–522[CrossRef]
    [Google Scholar]
  28. Paulsson M., Ljungh A., Wadström T.. 1992; Rapid identification of fibronectin, vitronectin, laminin, and collagen cell surface binding proteins on coagulase-negative staphylococci by particle agglutination assays. J Clin Microbiol30:2006–2012
    [Google Scholar]
  29. Pei L., Palma M., Nilsson M., Guss B., Flock J. I.. 1999; Functional studies of a fibrinogen binding protein from Staphylococcus epidermidis. Infect Immun67:4525–4530
    [Google Scholar]
  30. Peters G., Locci R., Pulverer G.. 1982; Adherence and growth of coagulase-negative staphylococci on surfaces of intravenous catheters. J Infect Dis146:479–482[CrossRef]
    [Google Scholar]
  31. Rapley R., Walker M.. 1992; PCR screening of DNA cloned into polylinker-containing vectors with M13 sequencing primers. Biotechniques12:516
    [Google Scholar]
  32. Schägger H., von Jagow G.. 1987; Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem166:368–379[CrossRef]
    [Google Scholar]
  33. Schneewind O., Mihaylova-Petkov D., Model P.. 1993; Cell wall sorting signals in surface proteins of gram-positive bacteria. EMBO J12:4803–4811
    [Google Scholar]
  34. Switalski L. M., Ryden C., Rubin K., Ljungh A., Höök M., Wadström T.. 1983; Binding of fibronectin to Staphylococcus strains. Infect Immun42:628–633
    [Google Scholar]
  35. Timmerman C. P., Fleer A., Besnier J. M., De Graaf L., Cremers F., Verhoef J.. 1991; Characterization of a proteinaceous adhesin of Staphylococcus epidermidis which mediates attachment to polystyrene. Infect Immun59:4187–4192
    [Google Scholar]
  36. Vaudaux P. E., Francois P., Proctor R. A., McDevitt D., Foster T. J., Albrecht R. M., Lew D. P., Wabers H., Cooper S. L.. 1995; Use of adhesion-defective mutants of Staphylococcus aureus to define the role of specific plasma proteins in promoting bacterial adhesion to canine arteriovenous shunts. Infect Immun63:585–590
    [Google Scholar]
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