1887

Abstract

Antibiotics are commonly used as growth promoters in animal husbandry worldwide. This practice has been linked to the emergence of particular antibiotic-resistant bacteria, and is now controversial. In this study, the ability of growth-promoting antibiotics to induce Shiga toxin (Stx)-converting bacteriophages from Stx-producing (STEC) strains was investigated. Subinhibitory concentrations of the antibacterial growth promoters olaquindox, carbadox, tylosin and monensin were used for induction experiments. The amount of mature Stx-converting phage particles released from induced and non-induced cultures was determined, and the production of Stx was simultaneously measured by ELISA. Whereas the quinoxaline-1,4-dioxide-type antibiotics olaquindox and carbadox enhanced the release of Stx-converting phage particles from STEC cells, tylosin and monensin decreased phage induction. The production of Stx increased or decreased simultaneously with the amount of free phages. The results of this study show that particular antibacterial growth promoters can induce Stx phages. induction of Stx phages from lysogenic STEC may increase the amount of free phages in the intestine and therefore may contribute to the spread of STEC and development of new STEC pathotypes.

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2000-05-01
2024-04-26
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References

  1. Beutin L., Geier D., Zimmermann S., Karch H. 1995; Virulence markers of Shiga-like toxin-producing Escherichia coli strains originating from healthy domestic animals of different species. J Clin Microbiol 33:631–635
    [Google Scholar]
  2. Brunder W., Schmidt H., Frosch M., Karch H. 1999; The large plasmids of Shiga-toxin-producing Escherichia coli (STEC) are highly variable genetic elements. Microbiology 145:1005–1014 [CrossRef]
    [Google Scholar]
  3. CDC 1982; Isolation of E. coli O157:H7 from sporadic cases of hemorrhagic colitis – United States. Morb Mortal Wkly Rep 31:580–585
    [Google Scholar]
  4. Fuchs S., Mühldorfer I., Donohue-Rolfe A., Kerényi M., Emödy L., Rossen A., Alexiev R., Nenkov P., Hacker J. 1999; Influence of RecA on in vivo virulence and Shiga toxin 2 production in Escherichia coli pathogens. Microb Pathog 27:13–23 [CrossRef]
    [Google Scholar]
  5. de Graaf G. J., Jager L. P., Baars A. J., Spierenburg Th. J. 1988; Some pharmacokinetic observations of carbadox medication in pigs. Vet Q 10:34–41 [CrossRef]
    [Google Scholar]
  6. Greife H. A., Berschauer F. 1988; Leistungsförderer in der Tierproduktion: Stand und Perspektiven. Übers Tierernährg 16:27–78
    [Google Scholar]
  7. Grif K., Dierich M. P., Karch H., Allerberger F. 1998; Strain-specific differences in the amount of Shiga toxin released from enterohemorrhagic Escherichia coli O157 following exposure to subinhibitory concentrations of antimicrobial agents. Eur J Clin Microbiol Infect Dis 17:761–766 [CrossRef]
    [Google Scholar]
  8. Griffin P. M., Tauxe R. V. 1991; The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol Rev 13:60–98
    [Google Scholar]
  9. Gunzer F., Böhm H., Rüssmann H., Bitzan M., Aleksic S., Karch H. 1992; Molecular detection of sorbitol-fermenting Escherichia coli O157 in patients with hemolytic-uremic syndrome. J Clin Microbiol 30:1807–1810
    [Google Scholar]
  10. Jacewicz M. S., Acheson D. W., Binion D. G., West G. A., Lincicome L. L., Fiocchi C., Keusch G. T. 1999; Responses of human intestinal microvascular endothelial cells to Shiga toxins 1 and 2 and pathogenesis of hemorrhagic colitis. Infect Immun 67:1439–1444
    [Google Scholar]
  11. Matsushiro A., Sato K., Miyamoto H., Yamamura T., Honda T. 1999; Induction of prophages of enterohemorrhagic Escherichia coli O157:H7 with norfloxacin. J Bacteriol 181:2257–2260
    [Google Scholar]
  12. Moore P. R., Evenson A., Luckey T. D., McCoy E., Elvehjem C. A., Hart E. B. 1946; Use of sulfasuxidine, streptothricin and streptomycin in nutritional studies with the chick. J Biol Chem 16:437
    [Google Scholar]
  13. Mühldorfer I., Hacker J., Keusch G. T., Acheson D. W., Tschäpe H., Kane A. V., Ritter A., Ölschläger T., Donohue-Rolfe A. 1996; Regulation of the Shiga-like toxin II operon in Escherichia coli. Infect Immun 64:495–502
    [Google Scholar]
  14. National Committee for Clinical Laboratory Standards 1993; Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved Standard M7-A3 Villanova, PA: National Commitee for Clinical Laboratory Standards;
    [Google Scholar]
  15. Neely M. N., Friedman D. I. 1998; Functional and genetic analysis of regulatory regions of coliphage H-19B: location of Shiga-like toxin and lysis genes suggest a role for phage functions in toxin release. Mol Microbiol 28:1255–1267 [CrossRef]
    [Google Scholar]
  16. Ostroff S. M., Griffin P. M., Tauxe R. V., Shipman L. D., Greene K. D., Wells J. G., Lewis J. H., Blake P. A., Kobayashi J. M. 1990; A statewide outbreak of Escherichia coli O157:H7 infections in Washington State. Am J Epidemiol 132:239–247
    [Google Scholar]
  17. Pierard D., Muyldermans G., Moriau L., Stevens D., Lauwers S. 1998; Identification of new verocytotoxin type 2 variant B-subunit genes in human and animal Escherichia coli isolates. J Clin Microbiol 36:3317–3322
    [Google Scholar]
  18. Richter H., Klie H., Timm M., Gallien P., Steinrück H., Perlberg K. W., Protz D. 1997; Verotoxin-producing E. coli (VTEC) in feces from cattle slaughtered in Germany. Berl Münch Tierärztl Wochenschr 110:121–127
    [Google Scholar]
  19. Riedel-Caspary G. 1986; Leistungsförderer in der Schweinemast. DLG-Mitteilung 11:608–619
    [Google Scholar]
  20. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Schmidt H., Geitz C., Tarr P. I., Frosch M., Karch H. 1999a; Non-O157:H7 pathogenic Shiga toxin-producing Escherichia coli: phenotypic and genetic profiling of virulence traits and evidence for clonality. J Infect Dis 179:115–123 [CrossRef]
    [Google Scholar]
  22. Schmidt H., Scheef J., Huppertz H. I., Frosch M., Karch H. 1999b; Escherichia coli O157:H7 and O157:H- strains that do not produce Shiga toxin: phenotypic and genotypic characterization of isolates asscoiated with diarrhea and hemolytic-uremic syndrome. J Clin Microbiol 37:3491–3496
    [Google Scholar]
  23. Smith H. W., Green P., Parsell Z. 1983; Vero cell toxins in Escherichia coli and related bacteria: transfer by phage and conjugation and toxic action in laboratory animals, chickens and pigs. J Gen Microbiol 129:3121–3137
    [Google Scholar]
  24. Spierenburg Th. J., van Lenthe H., de Graaf G., Jager L. P. 1988; Liquid chromatographic determination of olaquindox in medicated feeds and in contents of porcine gastrointestinal tract. J Assoc Off Anal Chem 71:1106–1109
    [Google Scholar]
  25. Strockbine N. A., Marques L. R., Newland J. W., Smith H. W., Holmes R. K., O’Brien A. D. 1986; Two toxin-converting phages from Escherichia coli O157:H7 strain 933 encode antigenically distinct toxins with similar biologic activities. Infect Immun 53:135–140
    [Google Scholar]
  26. Strockbine N. A., Wells J. G., Bopp C. A., Barrett T. J. 1998; Overview of detection and subtyping methods. In Escherichia coli O157:H7 and Other Shiga Toxin-producing E. coli Strains pp. 331–356Edited by Kaper J. B., O’Brien A. D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  27. Trevena W. B., Hooper R. S., Wray C., Willshaw G. A., Cheasty T., Domingue G. 1996; Vero cytotoxin-producing Escherichia coli O157 associated with companion animals. Vet Rec 138:400
    [Google Scholar]
  28. Watanabe H., Wada A., Inagaki Y., Itoh K., Tamura K. 1996; Outbreaks of enterohaemorrhagic Escherichia coli O157:H7 infection by two different genotype strains in Japan, 1996. Lancet 348:831–832 [CrossRef]
    [Google Scholar]
  29. Williams J. M., Boyd B., Nutikka A., Lingwood C. A., Barnett F. D., Milford D. V., Taylor C. M. 1999; A comparison of the effects of verocytotoxin-1 on primary human renal cell cultures. Toxicol Lett 105:47–57 [CrossRef]
    [Google Scholar]
  30. Witte W. 1998; Medical consequences of antibiotic use in agriculture. Science 279:996–997 [CrossRef]
    [Google Scholar]
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