1887

Abstract

Proliferative enteropathy (PE) is a complex of diseases of commercial importance to the pig industry. The obligate intracellular bacterium is consistently associated with PE and pure cultures of this bacterium have been used to reproduce PE in pigs. In this study bacteria were purified directly from PE-affected tissue. DNA extracted from purified bacteria was used to construct a partial genomic library which was screened using sera from -immunized rabbits. Two seroreactive recombinant clones were identified, one of which expressed proteins of 10 and 60 kDa. The sequence of the insert from this clone, plSI-2, revealed ORFs with sequence similarity to the operon of , the 50S ribosomal proteins L21 and L27 of , a GTP-binding protein of and a possible protoporphyrinogen oxidase, HemK, of Primers designed from unique sequences from the plSI-2 insert amplified DNA from infected, but not non-infected, porcine ilea; the amplicon sequence obtained from tissue-cultured was identical to the corresponding sequence in plSI-2, confirming the origin of the clone. The sequence of GroEL and other GroEL sequences in the databases were used to construct a partial phylogenetic tree. Analysis of the GroEL sequence relationship suggested that is not significantly related to other organisms whose GroEL sequences are held in the databases and supports previous data from 16S sequence analyses suggesting that is a member of a novel group of enteric pathogens.

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1998-08-01
2021-08-02
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