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Volume 144, Issue 2

Internalization of Aeromonas hydrophila by fish epithelial cells can be inhibited with a tyrosine kinase inhibitor Free

Abstract

is a Gram-negative bacterium that is pathogenic in fish, causing motile aeromonad septicaemia. It can enter (invade) fish cells, and survive as an intracellular parasite. The host-pathogen interaction and signal transduction pathway were studied by screening signal transduction inhibitors using carp epithelial cells and a virulent strain of the bacterium, PPD134/91. Genistein, a tyrosine kinase inhibitor, postponed internalization of into host cells, suggesting that tyrosine phosphorylation plays a role in internalization. In contrast, staurosporine, a protein kinase C inhibitor, and sodium orthovanadate, a protein tyrosine phosphatase inhibitor, accelerated internalization of PPD134/91. Other virulent strains of were also examined and it is likely that all strains, irrespective of serogroup, use the same signalling pathway to facilitate bacterial uptake.

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Keyword(s): Aeromonas hydrophila , fish epithelial cells and tyrosine kinase
© Society for General Microbiology 1998
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1998-02-01
2024-04-26
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Internalization of Aeromonas hydrophila by fish epithelial cells can be inhibited with a tyrosine kinase inhibitor
Microbiology 144, 299 (1998); https://doi.org/10.1099/00221287-144-2-299
/content/journal/micro/10.1099/00221287-144-2-299
/content/journal/micro/10.1099/00221287-144-2-299
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