1887

Abstract

Summary: The spectinomycin (Sp) resistance determinant from was cloned into using the plasmid vector pIJ699. A plasmid, pDGL15, with a 3.65 kb insert from conferring resistance to Sp was isolated. DNA sequence analysis of the 3651 bp DNA insert revealed four open reading frames (ORFs). The amino acid sequence deduced from one ORF (SpcN) showed a high degree of similarity to an aminoglycoside phosphotransferase (StrN) and from a second one (SpcR) to a regulatory protein (StrR) of the streptomycin biosynthesis gene cluster from The two other ORFs were incomplete and the deduced amino acid sequences showed similarities to an amidinotransferase encoded in the streptomycin biosynthesis gene cluster of and to the transposase of respectively. Expression of the gene in under the control of promoter conferred Sp resistance to the cells. An enzymic assay confirmed that the gene product of is an ATP-dependent aminoglycoside phosphotransferase which phosphoryiates Sp and actinamine, the aminocyclitol moiety of Sp.

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1997-07-01
2021-05-13
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References

  1. Altenbuchner J., Viell P., Pelletier I. 1992; Positive selection vectors based on palindromic DNA sequences. Methods Enzymol 216:457–466
    [Google Scholar]
  2. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410
    [Google Scholar]
  3. Benveniste R., Davies J. 1973; Aminoglycoside antibiotic- inactivating enzymes in actinomycetes similar to those present in clinical isolates of antibiotic-resistant bacteria. Proc Natl Acad Sci USA 70:2276–2280
    [Google Scholar]
  4. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248–254
    [Google Scholar]
  5. Brink M. F., G” Verbeet M. P., de Boer H. A. 1994; Spectinomycin interacts specifically with the residues G1064 and C1192 in 16S rRNA, thereby potentially freezing this molecule into an inactive conformation. Nucleic Acids Res 22:325–331
    [Google Scholar]
  6. Brosius J., Dull T. J., Sleeter D. D., Noller H. F. 1981; Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli . J Mol Biol 148:107–127
    [Google Scholar]
  7. Chung C. T., Niemela S. L., Miller R. H. 1989; One step preparation of competent E. coli: transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci USA 86:2172–2175
    [Google Scholar]
  8. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
    [Google Scholar]
  9. Distler J., Piepersberg W. 1985; Cloning and characterization of a gene from Streptomyces griseus coding for a streptomycin- phosphorylating activity. FEMS Microbiol Lett 28:113–117
    [Google Scholar]
  10. Distler J., Ebert A., Mansouri K., Pissowotzki K., Stockmann M., 8i Piepersberg W. 1987; Gene cluster for streptomycin biosynthesis in Streptomyces griseus: nucleotide sequence of three genes and analysis of transcriptional activity. Nucleic Acids Res 15:8041–8056
    [Google Scholar]
  11. Haas M. J., Dowding J. E. 1975; Aminoglycoside-modifying enzymes. Methods Enzymol 43:611–628
    [Google Scholar]
  12. Heinzel P., Werbitzky O., Distler J., Piepersberg W. 1988; Asecond streptomycin resistance gene from Streptomyces griseus codes for streptomycin 3//-phosphotransferase. Relationship between antibiotic and protein kinase. Arch Microbiol 150:184–192
    [Google Scholar]
  13. Henikoff S., Henikoff J. G. 1994; Protein family classification based on searching a database of blocks. Genomics 19:97–107
    [Google Scholar]
  14. Hopwood D. A., Kieser T., Wright H. M., Bibb M. J. 1983; Plasmids, recombination and chromosome mapping in S. lividans 66. J Gen Microbiol 129:2257–2269
    [Google Scholar]
  15. Hopwood D. A., Bibb M. J., Chater R. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. (editors) 1985 Genetic Manipulation of Streptomyces: a Laboratory Manual Norwich: John Innes Foundation;
    [Google Scholar]
  16. Janssen G. R. 1993; Eubacterial, archaebacterial, and eucaryotic genes that encode leaderless mRNA. . In Industrial Microorganisms: Basic and Applied Molecular Genetics , pp. 59–67 . Edited by Baltz R. H., Hegemann G. D., Skatrud P. L. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Kieser T. 1984; Factors affecting the isolation of ccc-DNA from Streptomyces lividans and Escherichia coli . Plasmid 12:19–36
    [Google Scholar]
  18. Kieser T., Melton R. E. 1988; Plasmid pIJ699, a multicopy positive-selection vector for Streptomyces . Gene 65:83–91
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  20. Marsh L. J., Erfle M., Wykes E. J. 1984; The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32:48–1485
    [Google Scholar]
  21. Mason D. J., Dietz A., Smith R. M. 1961; Actinospectacin, a new antibiotic. I. Discovery and biological properties. Antibiot Chemother 11:118–122
    [Google Scholar]
  22. Mitscher L. A., Martin L. L., Feller D. R. 1971; The biosynthesis of spectinomycin. Chem Commun 1971:1541–1542
    [Google Scholar]
  23. Neumann T., Piepersberg W., Distler J. 1996; Decision phase regulation of streptomycin production in Streptomyces griseus . Microbiology 142:1953–1963
    [Google Scholar]
  24. Okuda T., Ito Y. 1982; Biosynthesis and mutasynthesis of aminoglycoside antibiotics. . In Aminoglycoside Antibiotics , pp. 111–203 . Edited by Umezawa H., Hooper I. R. Berlin: Springer;
    [Google Scholar]
  25. Oliver P. J., Goldstein A., Bower R. R., Holper J. C., Otto R. H. 1962; M-141, a new antibiotic. I. Antimicrobial properties, identity with actinospectacin, and production by Streptomyces flavopersicus, sp. n. Antimicrob Agents Chemother 1961:495–502
    [Google Scholar]
  26. Pederson A. H. B., Wiesner P. J., Holmes K. K., Johnson C. J., Turck M. 1972; Spectinomycin and penicillin G in the treatment of gonorrhea. A comparative evaluation. J Am Med Assoc 220:205–208
    [Google Scholar]
  27. Pelletier I., Altenbuchner J. 1995; A bacterial esterase is homologous with non-haem haloperoxidases and displays brominating activity. Microbiology 141:459–468
    [Google Scholar]
  28. Piendl W., Eichenseer C., Viell P., Altenbuchner J., Cullum J. 1994; Analysis of putative DNA amplification genes in the element AUDI of Streptomyces lividans 66. Mol Gen Genet 244:439–443
    [Google Scholar]
  29. Pinto-Alphandary H., Mabilat C., Courvalin P. 1990; Emergence of aminoglycoside resistance genes aadA and aadE in the genus Campylobacter . Antimicrob Agents Chemother 34:1294–1296
    [Google Scholar]
  30. Pissowotzki K., Mansouri K., Piepersberg W. 1991; Genetics of streptomycin production in Streptomyces griseus-. molecular structure and putative function of genes strELMBlN . Mol Gen Genet 231:113–123
    [Google Scholar]
  31. Polsinelli M., Beretta M. 1966; Genetic recombination in crosses between Streptomyces aureofaciens and Streptomyces rimosus . J Bacteriol 91:63–68
    [Google Scholar]
  32. Retzlaff L., Distler J. 1995; The regulator of streptomycin gene expression, StrR, of Streptomyces griseus is a DNA binding activator protein with multiple recognition sites. Mol Microbiol 18:151–162
    [Google Scholar]
  33. Rodicio M. R., Alvarez M. A., Chater K. F. 1991; Isolation and genetic structure of IS112, an insertion sequence responsible for inactivation of the Sail restriction-modification system of Streptomyces albus G. Mol Gen Genet 225:142–147
    [Google Scholar]
  34. Rosenberg A. H., Lade B. N., Chui D., Lin S.-W., Dunn J. J., Studier F. W. 1987; Vectors for selective expression of cloned DNAs by T7 RNA polymerase. Gene 56:125–135
    [Google Scholar]
  35. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  36. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
    [Google Scholar]
  37. Schermerhorn P. G., Chu P.-S., Kijak P. J. 1995; Determination of spectinomycin residues in bovine milk using liquid chromatography with electrochemical detection. J Agric Food Chem 43:2122–2155
    [Google Scholar]
  38. Sedlmeier R., Altenbuchner J. 1992; Cloning and DNA sequence analysis of the mercury resistance genes of Streptomyces lividans . Mol Gen Genet 236:76–85
    [Google Scholar]
  39. Skeggs P. A., Holmes D. J., Cundliffe E. 1987; Cloning of aminoglycoside-resistance determinants from Streptomyces tenebrarius and comparison with related genes from other organisms. J Gen Microbiol 133:915–923
    [Google Scholar]
  40. Smith G. E., Sommers M. D. 1980; The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl- paper. Anal Biochem 109:123–129
    [Google Scholar]
  41. Tohyama H., Okami Y., Umezawa H. 1987; Nucleotide sequence of the streptomycin phosphotransferase and amidinotransferase from Streptomyces griseus . Nucleic Acids Res 15:1819–1834
    [Google Scholar]
  42. Vujaklija D., Horinouchi S., Beppu T. 1993; Detection of an A- factor-responsive protein that binds upstream activation sequence of strR, a regulatory gene for streptomycin biosynthesis in Streptomyces griseus . J Bacteriol 175:2652–2661
    [Google Scholar]
  43. Walker J. B. 1995; Enzymatic synthesis of aminocyclitol moieties of aminoglycoside antibiotics from inositol by Streptomyces spp. : detection of glutamine-aminocyclitol aminotransferase and diaminocyclitol aminotransferase activities in a spectinomycin producer. J Bacteriol 177:818–822
    [Google Scholar]
  44. Wiley P. F., Argoudelis A. D., Hoeksema H. 1963; The chemistry of actinospectacin. IV. The determination of the structure of actinospectacin. J Am Chem Soc 85:2652–2659
    [Google Scholar]
  45. Yamamoto H., Maurer K., Hutchinson C. R. 1986; Transformation of Streptomyces erythreus . J Antibiot 39:1304–1313
    [Google Scholar]
  46. Yamamoto M., Okachi R., Takasawa S., Kawamoto I., Kumakawa M., Sato S., 8t Nara T. 1974; Production of spectinomycin by a new subspecies of Streptomyces hygroscopicus . J Antibiot 27:79–80
    [Google Scholar]
  47. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
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